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[1]陈荣剑,祝仲珍,王占科,等.亚最低杀菌浓度阿莫西林对体外诱导金黄色葡萄球菌标准菌株的耐药研究[J].医学研究与战创伤救治(原医学研究生学报),2015,17(05):458-460，495.[doi:10.3969/j.issn.1672-271X.2015.05.003]
　CHEN Rong-jian,ZHU Zhong-zhen,WANG Zhan-ke,et al.Study on the antibiotic resistance of staphylococcus aureus induced by sub-MBC of amoxicillin in vitro[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2015,17(05):458-460，495.[doi:10.3969/j.issn.1672-271X.2015.05.003]
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亚最低杀菌浓度阿莫西林对体外诱导金黄色葡萄球菌标准菌株的耐药研究()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第17卷
期数:
2015年05期
页码:
458-460，495
栏目:
出版日期:
2015-09-30

文章信息/Info

Title:
Study on the antibiotic resistance of staphylococcus aureus induced by sub-MBC of amoxicillin in vitro
作者:
陈荣剑1; 祝仲珍2; 王占科3; 杨莉萍2; 袁小兰4; 胡新华2; 宁丽萍3; 兰小鹏5
330002 江西南昌，解放军94医院，1.普外科，2.感染管理科，3.检验科；4.330006 江西南昌，江西省肺科医院护理部；5.350025 福建福州，南京军区福州总医院检验科
Author(s):
CHEN Rong-jian1;  ZHU Zhong-zhen2;  WANG Zhan-ke3;  YANG Li-ping2;  YUAN Xiao-lan4;  HU Xin-hua2;  NING Li-ping3;  LAN Xiao-peng5.
1.Department of General Surgery， 2.Department of Nosocomiology， 3.Department of Clinic Laboratory， 94 Hospital of PLA， Nanchang， Jiangxi 330002， China； 4.Department of Nursing， Lung Hospital of Jiangxi Province， Nanchang， Jiangxi 330006， China； 5.Department of Clinic Laboratory， Fuzhou General Hospital of Nanjing Military Region， Fuzhou， Fujian 350025， China
关键词:
亚最低杀菌浓度; 阿莫西林; 体外诱导; 细菌耐药; 金黄色葡萄球菌
Keywords:
sub-MBC;  amoxicillin;  induce in vitro;  bacteria antibiotic;  staphylococcus aureus
分类号:
R969.3
DOI:
10.3969/j.issn.1672-271X.2015.05.003
文献标志码:
A
摘要:
目的 观察亚最低杀菌浓度(Sub-minimum bactericidal concentration，Sub-MBC)阿莫西林对金黄色葡萄球菌标准菌株的耐药性影响。方法 以金黄色葡萄球菌标准菌株（ATCC 29213）为研究对象，阿莫西林为抗菌药物，以微量稀释法定量检测不同诱导天数MBC值，采用1/2MBC浓度对金黄色葡萄球菌进行30 d多步体外诱导实验，观察金黄色葡萄球菌标准菌株MBC变化，以全自动微生物药敏鉴定仪对最终诱导后菌株进行阿莫西林耐药性分析。结果 金黄色葡萄球菌标准菌株MBC值阿莫西林体外诱导7 d即显著升高，诱导30 d升高至原始菌株MBC的32倍；经全自动药敏分析仪鉴定诱导出的耐药菌株对阿莫西林耐药。结论 亚最低杀菌浓度阿莫西林可体外诱导金黄色葡萄球菌标准菌株出现耐药，且耐药性随亚最低杀菌浓度诱导时间增加而升高，提示合理选择抗菌药物使用剂量可作为预防细菌耐药重要措施。
Abstract:
Objective To observe the effects of inducing by amoxicillin with the dose of sub-MBC(minimum bactericidal concentration) on the antibiotic resistance of Standard strains of staphylococcus aureus. Methods The standard strain ATCC 29213 of staphylococcus aureus were induced by amoxicillin with the dose of 1/2MBC for 30 days. The quantitative data of MBC were detected by microdilution method in vitro everyday， and the antibiotic resistance to amoxicillin of staphylococcus aureus induced by this method were identified using automatic microorganisms drug sensitivity analyzer. Results The quantitative data of MBC of the standard strain of staphylococcus aureus induced by amoxicillin for 7 days significantly increased， and the MBC increased 32 times after induced for 30 day than that of the original strain. The drug-resistant strain induced by our methods showed resistant of amoxicillin which was identified by the automatic microorganisms drug sensitivity analyzer. Conclusion Amoxicillin can induce the standard strains of staphylococcus aureus resistance to antibiotic with the dose of sub-MBC for long time in vitro， and the degree of the antibiotic resistance increased by the time of inducement with sub-MBC， suggesting that it is important to to select a rational dosage of antimicrobial to prevent antibiotic resistance in clinics.

参考文献/References:

[1]石晓卉，刘 琪，于湘友，等.外科重症监护室临床细菌分布及耐药性监测[J].东南国防医药，2014，16(4):349-352.
[2]肖舒心，徐晓刚.最具威胁性的超级耐药菌[J].中国感染与化疗杂志，2014，14(6):473-473.
[3]Penwell WF，Shapiro AB，Giacobbe RA，et al.Molecular mechanisms of sulbactam antibacterial activity and resistance determinants in acinetobacter baumannii[J].Antimicrob Agents Chemother，2015，59(3):1680-1689.
[4]赵 磊，荆鹏伟.氟喹诺酮类药物体外诱导大肠埃希菌耐药性观察[J].郑州大学学报：医学版，2011，46(1):103-106.
[5]黄学忠，林佩佩，陈晓飞，等.1385株临床流行菌株调查及耐药分析[J].东南国防医药，2011，13(3):219-222.
[6]王凤玲，代荣琴，刘玉枝，等.2006～2011年金黄色葡萄球菌耐药情况分析[J].国际检验医学杂志，2014，35(8):975-976.
[7]周家军，梁蓓蓓，王 睿，等.利奈唑胺与万古霉素对耐甲氧西林金黄色葡萄球菌体外抗菌活性[J].中国临床药理学杂志，2010，26(8):593-596.
[8]王效雷，罗 婕，丁兆霞，等.119例导尿管相关性尿路感染的病原体分布及耐药性分析[J].东南国防医药，2015，17(1):46-48.
[9]庞载元，吴贤丽，敖茂程，等.14种市售抗菌药对金黄色葡萄球菌标准菌株的体外抑菌作用[J].中国执业药师，2014，(11):20-22.
[10]尚 红，王毓三，申子瑜.全国临床检验操作规程[M].北京：人民卫生出版社，2015：586-587.
[11] Ni W，Song X，Cui J，et al.Testing the mutant selection window hypothesis with escherichia coli exposed to levofloxacin in a rabbit tissue cage infection model[J].Eur J Clin Microbiol Infect Dis，2014，33(3):385-389.
[12]Jolivet-Gougeon A，Bonnaure-Mallet M.Biofilms as a mechanism of bacterial resistance[J].Drug Discov Today Technol，2014，11:49-56.
[13]黄梨莎.亚抑菌浓度抗菌药物对金黄色葡萄球菌耐药性及SOS基因表达的影响[D].山东大学，2011.

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备注/Memo:
江西省卫生厅资助课题（20092108）
更新日期/Last Update: 2015-09-20