|本期目录/Table of Contents|

[1]税迎春,夏欣一,章 琼,等.四氧嘧啶糖尿病妊娠大鼠二肽基肽酶4活性变化[J].医学研究与战创伤救治(原医学研究生学报),2015,17(06):568-571.[doi:10.3969/j.issn.1672-271X.2015.06.003]
　SHUI Ying-chun,XIA Xin-yi,ZHANG Qiong,et al.Changes of DPP4 enzyme activity in alloxan-induced diabetic pregnant rat[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2015,17(06):568-571.[doi:10.3969/j.issn.1672-271X.2015.06.003]
点击复制

四氧嘧啶糖尿病妊娠大鼠二肽基肽酶4活性变化()
分享到62.8K

《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第17卷
期数:
2015年06期
页码:
568-571
栏目:
出版日期:
2015-12-15

文章信息/Info

Title:
Changes of DPP4 enzyme activity in alloxan-induced diabetic pregnant rat
作者:
税迎春1; 夏欣一2; 章 琼1; 张 承1; 吴元赭1
210002 江苏南京，南京军区南京总医院,1.妇产科,2.全军检验医学研究所
Author(s):
SHUI Ying-chun1;  XIA Xin-yi2;  ZHANG Qiong1;  ZHANG Cheng1;  WU Yuan-zhe1.
1.Department of Gynaecology, 2. The Peoples Liberation Army Inspection Institute for Medical Research Nanjing, Nanjing General Hospital of Nanjing Mlitary Region, Nanjing, Jiangsu 210002, China
关键词:
四氧嘧啶; 糖尿病妊娠大鼠; 二肽基肽酶4; 活性GLP-1; 胰岛素
Keywords:
alloxan;  diabetic pregnant rat;  DPP4;  active GLP-1;  insulin
分类号:
R587.1
DOI:
10.3969/j.issn.1672-271X.2015.06.003
文献标志码:
A
摘要:
目的 观察四氧嘧啶糖尿病妊娠大鼠体内二肽基肽酶4（DPP4）酶活性变化。方法 建立四氧嘧啶糖尿病妊娠大鼠模型，检测大鼠血浆及部分组织中DPP4酶活性变化，同时检测血浆总胆固醇（TC）、三酰甘油（TG）、胰岛素和活性胰高血糖素样肽-1（GLP-1）水平变化。结果 四氧嘧啶糖尿病妊娠大鼠糖耐量和胰岛素耐量显著异常。血浆、肺、肝和回肠DPP4活性显著升高，其吸光度值分别由(0.10±0.01)、（0.16±0.02）、（0.15±0.01）和（0.17±0.01）升高到(0.20±0.04)、(0.18±0.03)、(0.19±0.02)和(0.19±0.02)。血浆胰岛素和活性GLP-1水平显著降低，分别由(1.22±0.41)ng/L 和(3.11±1.20) pmol/L下降到(0.71±0.31)ng/L(P<0.01)和(2.03±0.93)pmol/L(P<0.05),而血浆TC和TG水平显著升高，分别由(3.23±0.62)mmol/L和(1.01±0.10)mmol/L升高到(5.32±1.41)mmol/L(P<0.01)和(1.83±0.41)mmol/L(P<0.01)。结论 四氧嘧啶糖尿病妊娠大鼠糖耐量和胰岛素耐量显著异常，血脂升高，胰岛素降低，血浆及部分组织DPP4酶活性升高，从而导致血浆活性GLP-1水平下降。
Abstract:
Objective To study changes of DPP4 enzyme activity in alloxan induced diabetic pregnant rat. Methods Diabetic rat were induced by alloxan, DPP4 enzyme activities of plasma and some tissues were determined. At the same time, the plasma insulin, TG, TC and active GLP-1 levels were also determined. Results There were obvious impaired glucose tolerance and insulin tolerance in alloxan induced diabetic pregnant rat. In alloxan induced diabetic pregnant rat, DPP4 enzyme activities of the plasma, lung, liver and ileum increased from (0.10±0.01), (0.16±0.02), (0.15±0.01) and (0.17±0.01) to (0.20±0.04), (0.18±0.03), (0.19±0.02) and (0.19±0.02), respectively. At the same time, plasma insulin and active GLP-1 levels declined from (1.22±0.41) ng/L and (3.11±1.20) pmol/L to (0.71±0.31, P<0.01) ng/L and (2.03±0.93, P<0.05) pmol/L respectively, but plasma TC and TG increased from (3.23±0.62) mmol/L and (1.01±0.10) mmol/L to (5.32±1.41, P<0.01) mmol/L and (1.83±0.41, P<0.01) mmol/L respectively. Conclusion In alloxan induced diabetic pregnant rat, glucose tolerance and insulin tolerance were abnormal significantly, accompanied with hyperlipidemia and hypoinsulinemia. DPP4 enzyme activities of the plasma and some tissues increased, then caused plasma active GLP-1 level decreased significantly. The results of the study can provide the experimental basis for the pathogenic mechanism of gestational diabetes mellitus research.

参考文献/References:

[1] 刘率男，刘 泉，孙素娟，等. 二甲双胍对2型糖尿病KKAy小鼠胰岛β细胞功能的影响及初步作用机制探讨[J]. 药学学报，2014，49 (11): 1554-1562. [2] 程俊霖, 刘江慧, 胡云芳,等.DPPⅣ抑制剂对2型糖尿病的Ⅰ期临床研究[J]. 东南国防医药，2014，16(6):592-596. [3] 徐淑云，卞如濂，陈 修.药理实验方法学[M].3版.北京:人民卫生出版社， 2002:1006-1007. [4] De MI, Korom S, Van DJ，et al. CD26, let it cut or cut it down [J]. Immunol Today, 1999, 20(8): 367-375. [5] 杨 露，成 玮，丁长花，等.谷氨酰胺降低2型糖尿病患者血糖的机制探讨[J]. 东南国防医药，2015，17(2):127-129. [6] 师俊梅.妊娠期糖尿病患者的血糖控制效果对妊娠结局的影响[J]. 东南国防医药，2015，17(1):40-42. [7] Abd A, Tahrani MK, Piya AH, et al. Saxagliptin: a new DPP-4 inhibitor for the treatment of type 2 diabetes mellitus [J]. Adv Ther, 2009, 26(3): 249-262. [8] Argyrakopoulou G, Doupis J. DPP4 inhibitors: from Sitagliptin monotherapy to the new Alogliptin-Pioglitazone combination therapy [J]. Adv Ther, 2009, 26(3): 272-280. [9] Kim SJ, Nian C, Doudet DJ, et al. Inhibition of dipeptidyl peptidase IV with sitagliptin (MK0431) prolongs islet graft survival in streptozotocin-induced diabetic mice [J]. Diabetes,2008,57(5): 1331-1339. [10] Furuta Y, Horiguchi M, Sugaru E, et al. Chronic administration of DSP-7238, a novel, potent, specific and substrate-selective DPP IV inhibitor, improves glycaemic control and beta-cell damage in diabetic mice[J]. Diabetes Obes Metab,2010,12(5): 421-430. [11] Yasushi K,Takayuki K,Youichi S. et al. Increased plasma dipeptidyl peptidase IV (DPP IV) activity and decreased DPP IV activity of visceral but not subcutaneous adipose tissue in impaired glucose tolerance rats induced by high-Fat or high-sucrose diet [J].Biol Pharm Bull, 2009, 32(3): 463-467. [12] Jian Y, Campitelli J, Gang H, et al. Increase in DPP-IV in the intestine, liver and kidney of the rat treated with high fat diet and streptozotocin [J]. Life Sci, 2007, 81(4): 272-279. [13] Mannucci E, Pala L, Ciani S, et al. Hyperglycaemia increases dipeptidyl peptidase IV activity in diabetes mellitus[J]. Diabetologia, 2005, 48(6): 1168-1172. [14] McKillop AM, Duffy NA, Lindsay JR, et al. Decreased dipeptidyl peptidase-IV activity and glucagon-like peptide-1(7-36) amide degradation in type 2 diabetic subjects [J]. Diabetes Res Clin Pract, 2008, 79(1): 79-85. [15] Meneilly GS, Demuth HU, McIntosh CS, et al. Effect of ageing and diabetes on glucose-dependent insulinotropic polypeptide and dipeptidyl peptidase IV responses to oral glucose [J]. Diabet Med, 2000, 17（5）: 346-350. [16] Stefanovic V, Antic S, Mitic-Zlatkovic M, et al. Reversal of increased lymphocyte PC-1 activity in patients with type 2 diabetes treated with metformin [J]. Diabetes Metab Res Rev, 1999, 15（6）: 400-404. [17] Marguet D, Baggio I, Kobayashi T, et al. Enhanced insulin secretion and improved glucose tolerance in mice lacking CD26 [J]. Proc Natl Acad Sci, 2000, 97(12): 6874-6879. [18] 李益明.DPP-4抑制剂的独特作用机制:胰岛α、β细胞双调节作用[J].临床与药物,2014,11(7):21-23. [19] 郭 衔, 王 翼, 龙兆丰.口服降糖药二肽基肽酶Ⅳ(DPP-4)抑制剂的多器官保护作用[J].药学与临床研究,2014,22(6):524-526.

相似文献/References:

备注/Memo

备注/Memo:
江苏省科技厅省级条件建设与民生科技专项基金（BM2013058）
更新日期/Last Update: 2015-11-20