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[1]李晓红,丁进亚,刘 杰,等.基质辅助激光解析电离飞行时间质谱用于产碳青霉烯酶肺炎克雷伯菌ST11分型的研究[J].医学研究与战创伤救治(原医学研究生学报),2016,18(03):240-243.[doi:10.3969/j.issn.1672-271X.2016.03.005]
　LI Xiao-hong,DING Jin-ya,LIU Jie,et al.A studyof typing ST11 of klebsiella pneumoniae producing carbapenemases by matrix-assisted laser desorption ionization-time of flight mass spectrometry[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2016,18(03):240-243.[doi:10.3969/j.issn.1672-271X.2016.03.005]
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基质辅助激光解析电离飞行时间质谱用于产碳青霉烯酶肺炎克雷伯菌ST11分型的研究()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第18卷
期数:
2016年03期
页码:
240-243
栏目:
出版日期:
2016-06-30

文章信息/Info

Title:
A studyof typing ST11 of klebsiella pneumoniae producing carbapenemases by matrix-assisted laser desorption ionization-time of flight mass spectrometry
作者:
李晓红1; 丁进亚2; 刘 杰3; 公彦文4; 薛文成5; 赵 斌6
1. 453003河南新乡，新乡医学院第三附属医院检验科；2. 430070湖北武汉，广州军区武汉总医院检验科；3. 100026北京，北京军区总医院检验科；4. 260000山东济南，济南军区总医院检验科；5. 110016辽宁沈阳，沈阳军区总医院检验科；6. 210002江苏南京，南京军区南京总医院医务部
Author(s):
LI Xiao-hong1;  DING Jin-ya2;  LIU Jie3;  GONG Yan-wen4;  XUE Wen-cheng5;  ZHAO Bin6.
1. Clinical Laboratory, the Third Affiliated Hospital of Xinxiang Medical University, Xinxiang，Henan 453003, China;2.Clinical Laboratory, General Hospital of Guangzhou Military Area in Wuhan, Wuhan， Hubei 430070，China;3. Clinical Laboratory, General Hospital of Beijing Military Command, Beijing 100026，China;
关键词:
ST11型; 肺炎克雷伯菌; 碳青霉烯酶; MALDI-TOF MS
Keywords:
ST11;  klebsiella pneumonia;  carbapenemases; MALDI-TOF MS
分类号:
R378
DOI:
10.3969/j.issn.1672-271X.2016.03.005
文献标志码:
A
摘要:
目的 探讨基质辅助激光解析电离飞行时间质谱（MALDI-TOF MS）用于产碳青霉烯酶肺炎克雷伯菌ST11分型。方法 收集2012年10月－2014年10月5所医院的80株产碳青霉烯酶肺炎克雷伯菌菌株。经多位点序列分析（MLST），28株为ST11型，52株为其他分型（other sequence type, OST）。通过MSP dendrogram聚类分析、MALDI Biotyper建立数据库、ClinPro Tools建立模型3种方法研究ST11分型的可能性。结果 MSP dendrogram聚类分析在距离水平线1000处可将80株菌聚类分析为两类，敏感性为65.78%，特异性为92.85%。MALDI Biotyper数据库验证结果：18株ST11有1株菌分为OST，32株OST有2株菌被分为ST11，敏感性为89.47%，特异性为96.77%。ClinPro Tools用其余菌株验证模型全部正确，敏感性和特异性均为100%。结论 MALDI-TOF MS可通过3种方法区别ST11与OST，以ClinPro Tools软件建立模型的方法最可靠。
Abstract:
Objective To study the probability of typing ST11 of klebsiella pneumoniae producing carbapenemases by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Methods From October 2012 to October 2014, we collected 80 isolates of klebsiella pneumoniaes producing carbapenemases. We researched all isolates by MLST finding that 28 isolates were ST11 and 52 isolates were other sequence types. Through MSP dendrogram, creating databases by MALDI Biotyper and building models by ClinPro Tools to analysis the probability of typing ST11. Results At the distance level cutoff of 1000, 80 isolates were divided into two clusters. The susceptibility with MSP dendrogram was 65.78%, and the specificity was 92.85%. The results of testing databases were that 1 of 18 ST11 and 2 of 20 OST were false. The susceptibility and the specificity with MALDI Biotyper were respectively 89.47% and 96.77%. The results of checking model with remaining isolates by the ClinPro Tools were all correctly. The susceptibility and specificity were both 100%. Conclusion Through three methods,MALDI-TOF MS has detection performance for the ST11 and OST, but ClinPro Tools is the best.

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备注/Memo

备注/Memo:
辽宁省科技攻关计划（2011225021）
更新日期/Last Update: 2016-05-20