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[1]林青伟,宋景春,曾庆波,等.大黄素对脓毒症大鼠凝血紊乱治疗的作用[J].医学研究与战创伤救治(原医学研究生学报),2018,20(05):464-470.[doi:10.3969/j.issn.1672-271X.2018.05.004]
　LIN Qing-wei,SONG Jing-chun,ZENG Qing-bo,et al.Effect ofemodin on coagulation disorders of septic rats[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2018,20(05):464-470.[doi:10.3969/j.issn.1672-271X.2018.05.004]
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大黄素对脓毒症大鼠凝血紊乱治疗的作用()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第20卷
期数:
2018年05期
页码:
464-470
栏目:
出版日期:
2018-09-18

文章信息/Info

Title:
Effect ofemodin on coagulation disorders of septic rats
作者:
林青伟; 宋景春; 曾庆波; 钟林翠; 宋晓敏; 张昕
作者单位：330002南昌，解放军第九四医院重症医学科（林青伟、宋景春、曾庆波、钟林翠、宋晓敏、张昕 ）
Author(s):
LIN Qing-wei; SONG Jing-chun; ZENG Qing-bo;  ZHONG Lin-cui;  SONG Xiao-min; ZHANG Xin
（Intensive Care Unit，the 94th Hospital of PLA, Nanchang 330002, Jiangxi, China）
关键词:
大黄素; 脓毒症;  凝血;  大鼠
Keywords:
emodin; sepsis; coagulation;  rat
分类号:
R285.5
DOI:
10.3969/j.issn.1672-271X.2018.05.004
文献标志码:
A
摘要:
目的 研究大黄素对脓毒症大鼠凝血功能紊乱的治疗作用。方法 将90只健康的雌性SD（Sprague Dawley）大鼠按照随机数字表法随机分为假手术组、盲肠结扎组和大黄素组。收集盲肠结扎术后6、12、24h的凝血酶原时间(PT)、部分凝血活酶时间（APTT）、血栓弹力图（TEG）普通杯及功能性纤维蛋白原杯参数，包括凝血反应时间(R)、血块形成速率(K)、血块形成动力学(Angle)、血块最大强度(MA)、凝血综合指数(CI)以及纤维蛋白原功能（FLEV）。观察各组大鼠48h的生存率。结果 假手术组、盲肠结扎组和大黄素组的盲肠结扎术后48h生存率依次为100%、20%和40%。术后6h，盲肠结扎组的APTT较假手术组均显著延长，大黄素组APTT较盲肠结扎组明显缩短；术后6、12、24h，盲肠结扎组和大黄素组的PT较假手术组均明显延长,差异均有统计学意义(P<0.05)。TEG普通杯结果 显示盲肠结扎组中，K值在术后12h显著降低(P<0.05)，α角、MA值以及CI值在术后12和24h显著升高(P<0.05)。大黄素组中，MA值以及CI值在术后12和24h均显著升高(P<0.05)。与假手术组比较，盲肠结扎组的α角在术后12h显著升高(P<0.05)，MA值与CI值在术后6h显著降低(P<0.05)。TEG功能性纤维蛋白原杯结果 显示盲肠结扎组中，α角在术后12h 显著增大(P<0.05）；MA值与FLEV值均在术后24h显著升高(P<0.05）。与盲肠结扎组比较，大黄素组的α角在术后12和24h显著升高（P<0.05），FLEV值及MA值在术后24h均显著升高(P<0.05）。结论 脓毒症大鼠的整体凝血状态表现为低凝倾向，凝血因子功能减弱，血小板及纤维蛋白原先减弱后增强。大黄素能够改善脓毒症大鼠内源性凝血因子活性和纤维蛋白原功能，降低脓毒症大鼠48h病死率。
Abstract:
Objective To investigate the therapeuti effects of emodin on coagulation disorders in septic rats.Methods Ninety healthy female SD rats were randomly divided into sham operation(SO) group, cecal ligation(CL) group and emodin(Em) group At 6, 12, 24 and 48 h after SO ,prothrombin time (PT), partial thromboplastin time (APTT) live enzymes, thrombosis,TEG and functional parameters were collected, which including coagulation reaction time (R), blood clot formation rate (K), blood clot formation dynamics (Angle), blood clots maximum intensity (MA), coagulation index (CI) and fibrinogen (FLEV) function.Survival rates at 48h were observed in each group.Results Fourty-eight hours after cecal ligation，survival rates in the SO，CL and Em group were 100%，20% and 40%，respectively．At 6, 12 and 24 hours after operation, the APTT was significantly longer in CL group (P<0.05), while at 6 hours, APTT was significantly shorter in Em group than in CL group (P<0.05). At 6, 12, 24 hours after operation, the PT was significantly longer in CL group and Em group than in SO group (P<0.05), however, there was no significant difference between Em group and CL group (P>0.05). At 6, 12, 24 hours after operation, there was no significant difference in the CLgroupand Em group as compared with the SO group(P<0.05). EXTEG: The K value decreased significantly at 12 h of CL group. The Angle, MA, and CI significantly increased at 12 h and 24 h after operation. In Em group, and the Angle, MA value, and CI value increased significantly at 12 and 24 hours. Compared with the SO group, the angle was significantly increased at 12 hours while MA and CI value were significantly decreased at 6 hours in CL group. FIBTEG:In CL group, the Angle was significantly increased at 12 h. At 12 h after operation, the MA value and FLEV value were significantly increased. Compared with the CL group, the Angle was significantly increased at 12 h,the Angle, FLEV, and MA value were increased significantly at 24 h in the Em group.Conclusion The coagulation status of septic rats showed a tendency to hypocoagulability. The specific mechanism were weakened coagulation factor, fibrinogen and platelet function declined first and then increased. Emodin can improve endogenous coagulation factor activity and fibrinogen function in septic rats to reduce 48 h mortality of septic rats.

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备注/Memo

备注/Memo:
基金项目：江西省研究生创新专项资金项目（YC2016-S364）
更新日期/Last Update: 2018-09-20