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[1]陈娟,刘传丽,陈艳思,等.IL-17A在卵巢癌中的表达水平及潜在机制的生物信息学分析[J].医学研究与战创伤救治(原医学研究生学报),2020,22(01):25-29.[doi:10.3969/j.issn.1672-271X.2020.01.006]
　CHENJuan,LIUChuan-li,CHENYan-si,et al.The expression level of IL-17A in ovarian cancer and the bioinformatics analysis of potential mechanism[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2020,22(01):25-29.[doi:10.3969/j.issn.1672-271X.2020.01.006]
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IL-17A在卵巢癌中的表达水平及潜在机制的生物信息学分析()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第22卷
期数:
2020年01期
页码:
25-29
栏目:
临床研究
出版日期:
2020-01-06

文章信息/Info

Title:
The expression level of IL-17A in ovarian cancer and the bioinformatics analysis of potential mechanism
作者:
陈娟; 刘传丽; 陈艳思; 王振国; 孔祥玲
作者单位：300162天津，武警特色医学中心妇产科（陈 娟、刘传丽、陈艳思、孔祥玲），医研部（王振国）
Author(s):
CHEN　Juan1;  LIU　Chuan-li1;  CHEN　Yan-si1;  WANG　Zhen-guo2;  KONG　Xiang-ling1
（1.Department of Obstetrics and Gynecology, 2. Medical and Research Department, Characteristic Medical Center of PAP, Tianjin 300162, China）
关键词:
白细胞介素?17A; 卵巢癌; 卵巢浆液性囊腺癌; 生物信息学
Keywords:
interleukin-17A;  ovarian cancer;  ovarian serous cystadenocarcinoma;  bioinformatics
分类号:
R711.75
DOI:
10.3969/j.issn.1672-271X.2020.01.006
文献标志码:
A
摘要:
目的　探讨白细胞介素-17A（IL-17A）在卵巢癌组织中表达水平的变化及潜在机制。 方法　选取武警特色医学中心2018 年1月至11月间收治的原发性卵巢浆液性囊腺癌患者20例，采用ELISA法检测肿瘤组织及癌旁正常组织IL-17A表达水平。下载肿瘤基因组图谱（TCGA）数据库中549例卵巢浆液性囊腺癌数据，按照IL-17A表达水平由高到低排序，将排在前50%的患者设为高表达组，剩余患者设为低表达组，绘制2组患者生存曲线；利用在线分析工具（http://www.linkedomics.org）将数据库中每一种基因的表达水平逐一与IL-17A表达水平进行Pearson相关性检测，根据相关系数选取100个相关基因，使用DAVID工具完成基因本体（GO）富集分析和京都基因与基因组百科全书通路（KEGG通路）富集分析。 结果　癌组织IL-17A表达水平显著高于癌旁正常组织[（7.23±2.11）ng/L vs （4.19±1.33）ng/L，P<0.05]；IL-17A高表达组中位生存时间高于IL-17A低表达组（1451 d vs 1348 d，风险比=0.74，95%CI为0.60～0.92，P<0.05）；在检测的5309个基因中，有2103个基因与IL-17A存在相关性（P<0.05），其中钙电压门控通道亚单位α1（CACNA1S）与IL-17A表达水平相关系数最高（r=0.6928，P<0.05)；GO富集分析和KEGG通路富集分析可见，相关基因主要涉及的生物学过程包括生物调节、刺激反应和多细胞生物过程，主要涉及的细胞组分为细胞膜、细胞核和囊泡，主要涉及的分子功能为蛋白质结合、离子结合和转运活性，主要涉及的信号通路为泛醌10和其他萜类醌的生物合成、ATP绑定蛋白转运和类固醇生物合成。 结论　IL-17A可作为评价卵巢浆液性囊腺癌预后的指标之一，这可能与其调控细胞膜表面电压门控通道及萜类醌的生物合成有关。
Abstract:
Objective　To investigate the changes and potential mechanisms of interleukin-17A (IL-17A) expression in ovarian cancer tissues.　Methods　Twenty patients with ovarian serous cystadenocarcinoma admitted to our hospital from January 2018 to November 2018 were enrolled. The expression of IL-17A in tumor tissues and adjacent normal tissues was detected by ELISA. 549 cases of ovarian serous cystadenocarcinoma in the cancer genome atlas (TCGA) database were downloaded. According to the order of IL-17A expression level from high to low, the patients in the top 50% were classified as high expression group, and the remaining patients were subdivided into low expression group. The survival curves of the two groups were drawn. Using an online analysis tool (http://www.linkedomics.org) to detect the Pearson correlation between the expression level of each gene in the database and the expression level of IL-17A one by one. According to the correlation coefficient, 100 genes were selected, and the gene ontology (GO) enrichment analysis and the Kyoto Gene and Genome Encyclopedia pathway (KEGG pathway) enrichment analysis were completed by the DAVID tool.　Results　The expression level of IL-17A in cancer tissues was significantly higher than that in adjacent normal tissues. The difference was statistically significant [(7.23±2.11)ng/L vs (4.19±1.33)ng/L, P<0.05]. The median survival time in high expression group was longer than that of low expression group (1451 d vs 1348 d, HR=0.74, 95%CI=0.60-0.92, P<0.05). Among the 5309 genes detected, 2103 genes existed correlation with IL-17A (P<0.05), in which the correlation coefficient of calcium voltage-gated channel subunit α1 (CACNA1S) and IL-17A expression level was the highest (r=0.6928, P<0.05).The GO enrichment analysis and the KEGG pathway enrichment analysis showed that the mainly involved biological processes are biological regulation, stimulated response and multicellular biological processes; the mainly involved components are cell membrane, nucleus and vesicles; the mainly involved molecules functions are protein binding, ionic binding and transport activity; the main signaling pathways are biosynthesis of ubiquinone 10 and other quinones, adenosine triphosphate (ATP)-binding protein transport and steroid biosynthesis.　Conclusion　IL-17A can be used as one of the prognostic indicators for evaluating ovarian serous cystadenocarcinoma, which may be related to regulation of cell surface voltage gated channels and biosynthesis of terpenoid quinones.

参考文献/References:

[1] 伍筱玫, 雷月, 段晓义. 程序性死亡受体-1/配体-1在卵巢癌中的研究进展[J]. 医学研究生学报, 2017,30(1):108-112. [2] 杨帆, 谢树红, 黄惠娟. 卵巢癌在代谢组学中的研究进展[J]. 东南国防医药, 2018,20(2):160-163. [3] 洪丽君, 曾清芳, 卢昆林, 等. 高尔基体磷蛋白3在卵巢癌中的表达及临床意义[J]. 东南国防医药, 2017,19(4):357-360. [4] Santibanez JF, Bjelica S. Novel Patents Targeting Interleukin-17A; Implications in Cancer and Inflammation[J]. Recent Pat Anticancer Drug Discov, 2018,13(2):133-144. [5] Liu Q, Li H, Yang J, et al. Valproic acid attenuates inflammation of optic nerve and apoptosis of retinal ganglion cells in a rat model of optic neuritis[J]. Biomed Pharmacother, 2017,96:1363-1370. [6] Li TJ, Jiang YM, Hu YF, et al. Interleukin-17-Producing Neutrophils Link Inflammatory Stimuli to Disease Progression by Promoting Angiogenesis in Gastric Cancer[J]. Clin Cancer Res, 2017,23(6):1575-1585. [7] 熊雯, 周新娥, 黄娟, 等. 血清 CA125、HE4及 IL-17检测对上皮性卵巢癌的早期诊断价值分析[J]. 中国计划生育和妇产科, 2016,8(3):69-73. [8] 张蓉, 梅红, 聂亚红, 等. Th17细胞及IL-17在卵巢癌患者外周血中的表达及临床意义[J]. 中国实验诊断学, 2016,20(7):1103-1105. [9] Vasaikar SV, Straub P, Wang J, et al. LinkedOmics: analyzing multi-omics data within and across 32 cancer types[J]. Nucleic Acids Res, 2018,46(D1):D956-956D963. [10] Huang YS, Guilleminault C, Hwang FM, et al. Inflammatory cytokines in pediatric obstructive sleep apnea[J]. Medicine (Baltimore), 2016,95(41):e4944. [11] 田泾, 高申. 基于疾病微环境靶向的精准纳米靶向技术研究[J]. 医学研究生学报, 2019,32(5):479-483. [12] 张海佳, 沙素梅, 徐斌, 等. IL-17在黏附侵袭性大肠杆菌感染小鼠结肠过程中的作用机制研究[J]. 现代生物医学进展, 2017,17(17):3211-3215,3205. [13] 陈献, 张晓洁, 尚文雯, 等. IL-17A在卵巢癌组织中的表达及临床意义[J]. 免疫学杂志, 2018,34(6):529-533. [14] 熊雯, 周新娥, 黄娟, 等. 血清CA125、HE4及IL-17检测对上皮性卵巢癌的早期诊断价值分析[J]. 中国计划生育和妇产科, 2016,8(3):69-73. [15] Wu X, Yang T, Liu X, et al. IL-17 promotes tumor angiogenesis through Stat3 pathway mediated upregulation of VEGF in gastric cancer[J]. Tumour Biol, 2016,37(4):5493-5501. [16] Numasaki M, Fukushi J, Ono M, et al. Interleukin-17 promotes angiogenesis and tumor growth[J]. Blood, 2003,101(7):2620-2627. [17] 王艳军, 王瑞玲, 张小丽. 人附睾分泌蛋白4、糖类抗原125及卵巢恶性肿瘤风险预测模型在卵巢癌诊断中的价值[J]. 武警后勤学院学报（医学版）, 2017,26(5):436-438. [18] 魏泰, 吴立玲, 俞光岩. 白介素-17在恶性肿瘤发生发展过程中作用及其机制的研究进展[J]. 生理科学进展, 2016,47(5):335-339. [19] Hutter C, Zenklusen JC. The Cancer Genome Atlas: Creating Lasting Value beyond Its Data[J]. Cell, 2018,173(2):283-285. [20] Lan C, Huang X, Lin S, et al. High density of IL-17-producing cells is associated with improved prognosis for advanced epithelial ovarian cancer[J]. Cell Tissue Res, 2013,352(2):351-359. [21] 何妍妍. 低钾型周期性麻痹的临床特点及散发病例中CACNA1S和SCN4A基因筛查[D]. 湖南:南华大学, 2017:12-16. [22] 范凌晔, 毛熙光. 中电导钙激活钾离子通道在肿瘤中的作用[J]. 西南军医, 2012,14(2):328-329. [23] 李刚. 辅酶Q10防治心脑血管疾病[J]. 中老年保健, 2010,13(8):52-52. [24] 李瑶琪, 杨敏, 李晓天. 铁死亡的生化过程及对肿瘤的影响[J]. 中国生物化学与分子生物学报, 2018,34(8):838-843. [25] Basuli D, Tesfay L, Deng Z, et al. Iron addiction: a novel therapeutic target in ovarian cancer[J]. Oncogene, 2017,36(29):4089-4099.

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备注/Memo

备注/Memo:
基金项目：天津市科技计划项目（16ZXHLSY00120）
更新日期/Last Update: 2020-01-06