[1]李树雯,俞琦,杨凤娇,等.卡介菌多糖核酸注射液对慢性阻塞性肺疾病大鼠血清ICAM-1、TNF-α和IL-2的影响[J].医学研究与战创伤救治(原医学研究生学报),2020,22(6):578-580.[doi:10.3969/j.issn.1672-271X.2020.06.005]
　LI Shu-wen,YU Qi,YANG Feng-jiao,et al.Effects of pcg-pnsinjection on ICAM-1,TNF-α and IL-2 in serums of rats with COPD[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2020,22(6):578-580.[doi:10.3969/j.issn.1672-271X.2020.06.005]
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卡介菌多糖核酸注射液对慢性阻塞性肺疾病大鼠血清ICAM-1、TNF-α和IL-2的影响()

《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第22卷
期数:
2020年6期
页码:
578-580
栏目:
基础研究
出版日期:
2020-11-20

文章信息/Info

Title:
Effects of pcg-pnsinjection on ICAM-1,TNF-α and IL-2 in serums of rats with COPD
作者:
李树雯; 俞琦; 杨凤娇; 李良红
作者单位：310007杭州，空军杭州特勤疗养中心康复疗养科（李树雯、俞琦、杨凤娇、李良红）
Author(s):
LI Shu-wen; YU Qi; YANG Feng-jiao; LI Liang-hong
（Department of Rehabilitation,Hangzhou Special Service Center of the Air Force,Hangzhou 310007,Zhejiang,China）
关键词:
细胞间黏附因子-1; 肿瘤坏死因子-α; 白细胞介素-2; 慢性阻塞性肺疾病; 卡介菌多糖核酸注射液
Keywords:
ICAM-1;  TNF-α;  IL-2;  chronic obstructive pulmonary disease;  BCG polysaccharide and nucleic injection
分类号:
R320.2415
DOI:
10.3969/j.issn.1672-271X.2020.06.005
文献标志码:
A
摘要:
目的通过观察卡介菌多糖核酸注射液对慢性阻塞性肺疾病（COPD）大鼠血清中ICAM-1、TNF-α、IL-2的影响，探讨卡介菌多糖核酸注射液治疗COPD的作用机制。方法随机将60只SD大鼠（雄性）分为对照组、模型组、药物组，每组20只。模型组和药物组采用熏香烟加气管内注入脂多糖法建立大鼠COPD模型，药物组给予卡介菌多糖核酸注射液0.45 mg/kg腹腔注射，隔日1次。模型组、对照组给予等渗盐水0.3 mL腹腔注射，隔日1次。采用双抗夹心ELISA方法检测大鼠血清ICAM-1、TNF-α、IL-2含量。结果模型组大鼠血清中的ICAM-1、TNF-α、IL-2[（281.3±48.3）、（383.9±210.0）、（4084.1±2408.9）ng/mL]较对照组[（197.2±33.4）、（229.5±61.7）、（695.2±466.7）ng/mL]显著升高（P<0.05），而药物组大鼠血清中的ICAM-1、TNF-α、IL-2[（237.2±33.4）、（252.2±160.2）、（2084.0±2002.7）ng/mL]含量较模型组明显降低（P<0.05）。结论卡介菌多糖核酸注射液能降低COPD大鼠血清ICAM-1、TNF-α、IL-2含量，调节机体免疫功能，抑制组织炎症反应。
Abstract:
ObjectiveTo observe the effects of BCG polysaccharide and nucleic injection on ICAM-1, TNF-α and IL-2 in the serum of rats with chronic obstructive pulmonary disease (COPD), and study the mechanism of preventing and treating COPD.Methods60 male SD rats were randomly divided into blank group, model group, treatment group. COPD models were established by pouring Lipopolysacchride (LPS) into trachea and put rats into smoking environment twice a day. Rats in treatment group were taken with PCG-PNS intraperitoneal injection for 30 days. The levels of ICAM-1, TNF-α and IL-2 in serums of rats in each group was measured with enzyme-linked immunosorbent assay（ELISA）.ResultsThe levels of ICAM-1, TNF-α and IL-2 in rat serum of the model group [（281.3±48.3）,（383.9±210.0）,（4084.1±2408.9）ng/mL] was significantly higher than that of the blank group [（197.2±33.4）,（229.5±61.7）,（695.2±466.7）ng/mL] (P<0.05). The levels of ICAM-1, TNF-α and IL-2 in rat serums of treatment group [（237.2±33.4）,（252.2±160.2）,（2084.0±2002.7）ng/mL] was obviously less than that of the model group (P<0.05).ConclusionThis study suggests that PCG-PNS injection can reduce the level of ICAM-1, TNF-αand IL-2 in serum of rats with COPD and regulate its immune function, indicating its effect on inhibition of tissue inflammation.

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备注/Memo:
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更新日期/Last Update: 2020-12-01