|本期目录/Table of Contents|

[1]王永杰,李晓洲,骆晓梅,等.库存全血血浆中性粒细胞肽和弹性蛋白酶含量的动态观察[J].医学研究与战创伤救治(原医学研究生学报),2008,10(01):7-9.
　WANG Yong-jie,LI Xiao-zhou,LUO Xiao-mei,et al.Dynamic observation of human neutrophil peptide and neutrophil elastase contents of preserved whole plasma[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2008,10(01):7-9.
点击复制

库存全血血浆中性粒细胞肽和弹性蛋白酶含量的动态观察()
分享到62.8K

《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第10卷
期数:
2008年01期
页码:
7-9
栏目:
出版日期:
2008-01-20

文章信息/Info

Title:
Dynamic observation of human neutrophil peptide and neutrophil elastase contents of preserved whole plasma
文章编号:
1672-271X(2008)01-0007-03
作者:
王永杰; 李晓洲; 骆晓梅; 徐 涛; 陶征中; 周 军; 张育才
解放军第97医院，江苏徐州 221004
Author(s):
WANG Yong-jie;  LI Xiao-zhou;  LUO Xiao-mei; XU Tao; TAO Zheng-zhong; ZHOU Jun; ZHANG Yu-cai
The 97th Hospital of PLA, Xuzhou 221004, Jiangsu,China
关键词:
中性粒细胞肽; 弹性蛋白酶; 全血
Keywords:
Neutrophil peptide;  Elastase;  Blood bank conventional preservation
分类号:
R457
DOI:
-
文献标志码:
A
摘要:
目的 观察血库常规保存的全血不同保存时间血浆中性粒细胞肽(HNP)和中性粒细胞弹性蛋白酶(NE)含量的动态变化，探讨不同保存时 间对多形核中性粒细胞(PMN)稳定性的影响。方法 使用ELISA夹心法，对36名健康献血者新鲜血浆和血库常规保存的9份全血不同保存时间血浆HNP和NE含量进行了定量测定。结果 36名健康献血者血浆HNP和NE含量分别为(46±48) ng/ml和(35±28) ng/ml；常规保存的9份全血随保存时间的延长血浆HNP和NE含量逐渐升高，保存到10天时的全血血浆HNP浓度和 保存到15天时的全血血浆NE浓度(77±43) ng/ml与第1天相比升高显著(P<0.01)，且HNP和NE之间的含量变化呈正相关(r=0.96，P<0.01)。结论 库存全血血浆中HNP和NE随保存时间 延长含量增加，可能是PMN胞浆成分逐渐进入血浆所致。
Abstract:
Objective To dynamic observe plasma human neutrophil peptide(HNP) and neutrophil elastase(NE) content and stability of PMN in different time of preservation. Methods According to the ELISA method, the HNP and NE content of 36 healthy plasma and 9 plasma of the blood bank conventional preservation were determined.Results The HNP contents of 36 healthy plasma were (46±48) ng/ml.The NE contents of 36 healthy plasma were (35±28) ng/ml. The HNP and NE contents of 9 plasma were increase along with the increased storage time for the blood bank conventional preservation. When saving to 10 days, there was significant difference in HMP content between the first day[(45±42) ng/ml] and the 10th day[(98±61) ng/ml] (P<0.01).When saving to 15 days, there was significant difference in NE content between the first day[(34±27) ng/ml] and the 15th day[(77±43) ng/ml](P<0.01).HNP presented positive related to the NE (r=0.96，P<0.01).Conclusion For reason that the PMN cytoplasmic components soak into plasma gradually,the plasma HNP and NE content is increase along with the increased storage time for the blood bank conventional preservation.

参考文献/References:

[1]Zhang H,Porro G, Orzech N, et al. Neutrophil defensins me- diate acute inflammatory response and lung dysfunction in dose-related fashion[J]. Am J Physiol Lung Cell Mol Physiol,2001，280(5):947-954.
[2]王殿华，刘 旭.中性粒细胞弹性蛋白酶与急性肺损伤[J].国外医学呼吸系统分册,2004,24(6):368-370.
[3]冼乐武.人类生物防御素与肺部炎症性疾病[J].国外医学呼吸系统分册，2002，22(4):197-200.
[4]Spencer LT, Paone G, Kerin PM,et al.Role of human neutro- phil peptides in lung inflammation associated with α1-antitrypsin deficiency[J]. Am J Physiol Lung Cell Mol Physiol,2004,286(3):514-520.
[5]Frass O M, Bühling F, T¨αger M, et al. Antioxidant and ant- iprotease status in peripheral blood and BAL fluid after cardiopulmonary bypass[J]. Chest，2001，120(5):1599-1608.
[6]王永杰,张育才,巩新建,等.体外循环围手术期患者血浆中性粒细胞肽和弹性蛋白酶的动态观察 [J]. 临床检验杂志，2006，24(6):422-424.
[7]王永杰，骆晓梅，李晓洲,等.检测人中性粒细胞防御素ELISA法的建立及初步应用[J].临床检验杂志，2005，23(6):421-422.
[8]Panyutich AV,panyutich EA,Krapivin VA,et al.Plasma def- ensin concentrations are elevated in patients with septicemia or bacterial meningitis[Ｊ]. J Lab Clin Med,1993,122(2):202-207.
[9]Christopher CS, Lynn KB, Zahra M, et al. Transfusion-rela- ted acute lung injury: epidemiology and a prospective analysis of etiologic factors[J]. Blood, 2003,101(2):454-462.
[10]Nishiyama T, Hanaoka K. Do the effects of a protease inhi- bitor, ulinastatin, on elastase release by blood transfusion depend on interleukin 6[J]? Crit Care Med，2001，29(11):2106-2110.
[11]Lee CT,Fein AM,Lippmann M,et al.Elastolytic activity in pulmonary lavage fluid from patients with adult respiratory distress syndrome[J].N Engl J Med,1981,304(4):192-196.
[12]Tomashefski Jr JF.Pulmonary pathology of acute respiratory distress syndrome[J]. Clin Chest Med,2000,21(3):435-466.

相似文献/References:

备注/Memo

备注/Memo:
江苏省高校自然科学研究计划项目(03KJD310237)
更新日期/Last Update: 2013-11-22