|本期目录/Table of Contents|

[1]毛 钊,毛 曦,储成林.人牙周膜细胞接种于纳米-羟基磷灰石上 的形态学研究[J].医学研究与战创伤救治(原医学研究生学报),2008,10(03):161.
 MAO Zhao,MAO Xi,CHU Cheng-lin.The study on morphology by seeding human periodontal ligament cells onto three-dimensional porous nano-hydroxylapatit[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2008,10(03):161.
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人牙周膜细胞接种于纳米-羟基磷灰石上 的形态学研究()

《医学研究与战创伤救治》(原医学研究生学报)[ISSN:1672-271X/CN:32-1713/R]

卷:
第10卷
期数:
2008年03期
页码:
161
栏目:
出版日期:
2008-05-20

文章信息/Info

Title:
The study on morphology by seeding human periodontal ligament cells onto three-dimensional porous nano-hydroxylapatit
文章编号:
1672-271X(2008)03-0161-03
作者:
毛 钊1毛 曦2储成林3
1.南京军区南京总医院口腔科,江苏南京 210002;2.南京大学生命科学院,江苏南京 210093;3.东南大学材料研究所,江苏南京 210096
Author(s):
MAO Zhao1MAO Xi2CHU Cheng-lin3
1.Department of Stomatology, Nanjing General Hospital of Nanjing Command, Nanjing 210002, Jiangsu, China; 2. Department of Biological Science and Technology, School of Life Sciences, Nanjing University, Nanjing 210093, Jiangsu, China; 3. Institute of Func
关键词:
人牙周膜细胞三维多孔纳米-羟基磷灰石支架材料牙周组织工程
Keywords:
Human periodontal ligament cells Three-dimensional porous nano-hydroxylapatite scaffoldsPeriodontal tissue engineering
分类号:
R783
DOI:
-
文献标志码:
A
摘要:
目的 观察人牙周膜细胞接种于三维多孔纳米-羟基磷灰石上的组织形态学表现。方法 将原代培养的人牙周膜细胞接种于三维多孔纳米-羟基磷灰石上,培养15天后,采用扫描电子显微镜观察细胞的生长情况,探讨两者的复合情况。结果 作为种子细胞的人牙周膜细胞接种到支架材料上后,细胞粘附充分,生长旺盛,并有钙结节和少量胶原纤维形成。结论 三维多孔纳米-羟基磷灰石支架材料是一种比较符合牙周组织工程学条件的支架材料。
Abstract:
Objective To observe the morphology by seeding human periodontal ligament cells onto three-dimensional porous nano-hydroxylapatite.Methods Human periodontal ligament cells were seeded onto three-dimensional porous nano-hydroxylapatite scaffolds,cultured for 15 days in vitro. Scanning electron microscopy was used to observe morphology of cells seeded on three-dimensional porous nano-hydroxylapatite scaffolds. Results The cells seeded on three-dimensional porous nano-hydroxylapatite scaffolds had sufficient adhesive behavior,proliferated well and produced many tiny calcium nodules and collagenous fibers. Conclusion The study demonstrats that three-dimensional porous nano-hydroxylapatite scaffolds may be considered as a suitable scaffold in periodontal tissue engineering.

参考文献/References:

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[7]Zhang YF, Cheng XR, Chen Y, et al. Three-dimensional nano-hydroxylapatite/chitosan scaffolds as potential tissue engineered periodontal tissue[J].J Biomater Appl, 2007,21(4):333-349.
[8]孙凯莹,孙卫兵,储成林,等. 纳米羟基磷灰石-壳聚糖复合材料对细胞粘附的影响[J].临床口腔医学杂志,2007,23(3):150-153.

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备注/Memo

备注/Memo:
江苏省科技厅高技术研究资助项目(BG2001033)
更新日期/Last Update: 2013-11-22