|本期目录/Table of Contents|

[1]姚仁南,张建辉,陈复兴,等.丙型肝炎病毒核心抗原检测试剂的 初步应用[J].医学研究与战创伤救治(原医学研究生学报),2008,10(03):168.
　YAO Ren-nan,Zhang Jian-hui,Chen Fu-xin,et al.Preliminary application of hepatitis C virus core antigen detection kit[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2008,10(03):168.
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丙型肝炎病毒核心抗原检测试剂的 初步应用()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第10卷
期数:
2008年03期
页码:
168
栏目:
出版日期:
2008-05-20

文章信息/Info

Title:
Preliminary application of hepatitis C virus core antigen detection kit
文章编号:
1672-271X(2008)03-0168-02
作者:
姚仁南1; 张建辉2; 陈复兴1; 黄晓静1; 赵 勤1; 江学成1
1.解放军第97医院，江苏徐州 221004；2.湖南景达基因有限公司，湖南长沙 410000
Author(s):
YAO Ren-nan1;  Zhang Jian-hui2; Chen Fu-xin1; HUANG Xiao-jing1; ZHAO Qin1; JIANG Xue-cheng1
1.The 97th Hospital of PLA，Xuzhou 221004，Jiangsu，China;2.Hunan Jingda Gene Co.LTD.,Changsha 410000,Hunan,China
关键词:
丙型肝炎; 核心抗原; 酶联免疫吸附试验
Keywords:
HCV; Core antigen; Enzyme-linked immunosorbent assay(ELISA)
分类号:
R512.6＋3
DOI:
-
文献标志码:
A
摘要:
目的 应用丙型病毒性肝炎核心抗原(HCV-cAg)ELISA检测试剂，检测丙型病毒性肝炎(HCV)病毒标志物。方法 对单项抗-HCV ELISA试剂A阳性15份和单项试剂B阳性17份血清标本分别再用HCV-cAg ELISA试剂和HCV RNA荧光定量RT-PCR试剂检测，对其余血清标本仅行荧光定量RT-PCR试剂检测。结果 对32份阳性血清标本中HCV-cAg ELISA试剂和HCV-PCR荧光定量法阳性率分别为18.75%(6/32)和15.6%(5/32)。结论 HCV-cAg ELISA法的敏感性与HCV RNA RT-PCR荧光定量检测结果相类似，有助于可疑抗HCV阳性结果的证实。
Abstract:
Objective Hepatitis C core antigen enzyme-linked immunosorbent assay reagents (HCV-cAg ELISA) was used to detect the virus markers of viral hepatitis C (HCV). Methods 15 serum samples that were A positive and 17 were B positive detected by anti-HCV ELISA reagents were checked by HCV-cAg ELISA and HCV RNA fluorescence quantitative RT-PCR. Other samples were tested only by fluorescence quantitative RT-PCR. Results 18.75% (6/32) and 15.6% (5/32) serum samples were detected positively by HCV-cAg ELISA and fluorescence quantitative PCR respectively. Conclusion The sensitivity of HCV-cAg ELISA is similar to that of HCV RNA fluorescence quantitative RT-PCR.

参考文献/References:

[1]Colin C,Lanoir D,Touzet S,et al.Sensitivity and specificity of third-generation hepatitis C virus antibody detection assay :an analysis of the literature [J].J Hepati,2001,8 (1):87-95.
[2]谢 立，吴晓东.丙型肝炎病毒检测方法的研究进展及其临床意义[J].世界华人消化杂志，2005，13(7):884-886.
[3]王国华，张贺秋，李少波,等.丙型肝炎病毒核心抗原检测试剂的研制及初步应用[J].中国输血杂志,2004，17(5):299-301.
[4]孟淑芳，李秀华，尹红章,等.丙型肝炎病毒抗原检测方法的建立[J].中华实验和临床病毒学杂志，2001，15(3):287-230.
[5]Muerhoff A,Jiang L,Shah D,et al.Detection of HCV core antigen in human serum and plasma with an automated chemiluminescent immunoassay[J]. Transfusion,2002,42(3):349-356.
[6]姚仁南，张建辉，黄晓静,等.在安全输血中应用丙型肝炎病毒核心抗原检测技术的初步探索[J]. 中国实验血液学杂志，2006,14(3):617-618.
[7]Widell A,Molnegren V,Pieksma F,et al.Detection of hepatitis C core antigen in serum or plasma as a marker of hepatitis C viraemia in the serological window phase [J]. Transfusion Med,2002,12(2):107-113.
[8]Peterson J,Green G,Iida K,et al.Detection of hepatitis C core antigen in the antibody negative window phase of hepatitis Cinfection[J].Vox Sang,2000,78,(2):80-86.

相似文献/References:

[1]龚建明,杨静静,王昊鹏,等.南京地区吸毒人群丙型肝炎的 常见基因型分型研究[J].医学研究与战创伤救治(原医学研究生学报),2009,11(03):199.
　GONG Jian-ming,YANG Jing-jing,WANG Hao-peng,et al.Genotype Study on Hepatitis C Virus among Drug Users in Nanjing, China[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2009,11(03):199.

备注/Memo

备注/Memo:
国科发计字[2004]449号
更新日期/Last Update: 2013-11-22