|本期目录/Table of Contents|

[1]阳艳丽,王自正.噬菌体展示技术体内筛选小鼠肾脏 特异性多肽[J].医学研究与战创伤救治(原医学研究生学报),2008,10(04):248.
　YANG Yan-li,WANG Zi-zheng.Selecting kidney specific peptides of mice by phage display in vivo[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2008,10(04):248.
点击复制

噬菌体展示技术体内筛选小鼠肾脏 特异性多肽()
分享到62.8K

《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第10卷
期数:
2008年04期
页码:
248
栏目:
出版日期:
2008-07-20

文章信息/Info

Title:
Selecting kidney specific peptides of mice by phage display in vivo
文章编号:
1672-271X(2008)04-0248-04
作者:
阳艳丽1; 王自正2
1.东南大学附属中大医院，江苏南京210009；2.南京医科大学附属南京市第一医院，江苏南京210006
Author(s):
YANG Yan-li1; WANG Zi-zheng2
1.Zhongda Hospital Affiliatied to Southeast University, Nanjing 210009,Jiangsu,China；2.Nanjing Clinical Nuclear Medicine Center, Nanjing First Hospital Affiliatied to Nanjing Medical University, Nanjing 210006, Jiangsu,China
关键词:
噬菌体体内展示; 肾脏特异性多肽; 多肽筛选; 随机多肽文库
Keywords:
Phage display in vivo; Kidney specific peptides; Peptide screening; Random peptide libraries
分类号:
Q78
DOI:
-
文献标志码:
A
摘要:
目的利用噬菌体随机多肽文库对小鼠进行体内筛选，获得小鼠肾脏血管特异性多肽。方法将噬菌体多肽文库经尾静脉注射入小鼠体内，循环10 min后进行心脏灌注，收获小鼠肾脏，经过洗涤研磨后得到与肾脏血管特异性结合的噬菌体，该噬菌体体外扩增后被用于下一轮筛选，三轮筛选后随机挑取24个阳性噬菌体克隆送测序，并分析这些序列之间的共同序列。结果经过三轮筛选，特异性结合于小鼠肾脏血管的噬菌体得到富集，测序结果显示多肽序列VSASYHR出现的概率最大 (62.5%), 其次为GQWGARG(25.0％)。结论利用噬菌体多肽文库对小鼠进行体内筛选可以得到小鼠肾脏血管特异性多肽。
Abstract:
ObjectiveSelecting kidney vascular specific peptides of mice in vivo with phage peptide libraries.MethodsAfter phage peptide library was injected in mouse by intravenous injection and heart perfusion was carried out after 10 minutes, kidney was harvested.Kidney vascular specific peptides which were used in the next screening were obtained after washing，grinding and amplification in vitro. After three circulations, DNAs were extracted from 24 single clones and were sequenced. Sequences were analysed to determine the collective sequence.ResultsAfter three circulations, phages specific to kidney vascular were enriched. The result of sequencing revealed that VSASYHR was most frequent (62.5%). Next was GQWGARG (25.0%).ConclusionIt is feasible to obtain kidney specific peptides with phage display in vivo.

参考文献/References:

[1]Nowakowski GS, Dooner MS,Valinski HM,et al.A specific heptapeptide from a phage display peptide library homes to bone marrow and binds to primitive hematopoietic stem cells［J］.Stem Cells,2004,22(6):1030-1038.
[2］Yao VJ,Ozawa MG,Trepel M,et al.Targeting pancreatic islets with phage display assisted by laser pressure catapult microdissection［J］.Am J Pathol,2005,166(2):625-636.
[3］Bockmann M,Drosten M,Putzer BM.Discovery of targeting peptides for selective therapy of medullary thyroid carcinoma［J］.J Gene Med,2005,7(2):179-188.
[4］Krag DN,Shukla GS,Shen GP,et al. Selection of tumor-binding ligands in cancer patients with phage display libraries［J］. Cancer Res,2006,66(15):7724-7733.
[5］Massoud TF,Gambhir SS.Molecular imaging in living subjects:seeing fundamental biological processes in a new light［J］.Genes Dev, 2003,17(5):545-580.
[6］Koivunen E,Arap W,Rajotte D,et al.Identification of receptor ligands with phage display peptide libraries［J］.J Nucl Med,1999,40(5):883-888.
[7］Yip YL, Hawkins NJ, Smith G, et al. Biodistribution of filamentous phage fab in nude mice［J］.J Immunol Methods,1999,225(1-2):171-178.
[8］Smith J, Kontermann RE, Embleton J, et al. Antibody phage display technologies with special reference to angiogenesis［J］. FASEB J,2005,19(3):331-341.
[9］Barth S, Huhn M, Matthey B, et al. Ki-4(scFv)-ETA, a new recombinant anti-CD30 immunotoxin with highly specific cytotoxic activity against disseminated Hodgkin tumors in SCID mice［J］.Blood, 2000, 95(12):3909-3914.

相似文献/References:

备注/Memo

备注/Memo:
-
更新日期/Last Update: 2013-11-22