|本期目录/Table of Contents|

[1]王小红,王军青,王志君,等.HLA-G在子痫前期患者与正常妊娠胎盘中的差异表达[J].医学研究与战创伤救治(原医学研究生学报),2010,12(01):18-20.
　WANG Xiao-hong,WANG Jun-qing,WANG Zhi-jun,et al.Differential expression of HLA-G in the placentae from pre-eclampsia and normal pregnant[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2010,12(01):18-20.
点击复制

HLA-G在子痫前期患者与正常妊娠胎盘中的差异表达()
分享到62.8K

《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第12卷
期数:
2010年01期
页码:
18-20
栏目:
出版日期:
2010-01-20

文章信息/Info

Title:
Differential expression of HLA-G in the placentae from pre-eclampsia and normal pregnant
文章编号:
1672-271X(2010)01-0018-03
作者:
王小红; 王军青; 王志君; 潘小红
214044江苏无锡，解放军101医院妇产科
Author(s):
WANG Xiao-hong; WANG Jun-qing; WANG Zhi-jun; PAN Xiao-hong.
The Obstetricse and Gynecology Department,101 Hospital of PLA,Wuxi,Jiangsu 214044，China
关键词:
子痫前期; 人类白细胞抗原-G; 胎盘; 免疫耐受
Keywords:
pre-eclampsia;  HLA-G;  placentae;  immunal tolance
分类号:
R714.24+5
DOI:
-
文献标志码:
A
摘要:
目的 通过检测人类白细胞相关抗原-G(HLA-G)在子痫前期患者与正常胎盘组织中的表达，探讨HLA-G在子痫前期中的表达意义。方法 采用半定量逆转录-聚合酶链技术(RT-PCR)及免疫印迹(Western blot)分析技术分别检测了24例子痫前期患者(观察组，轻度子痫前期12例，重度子痫前期12例)及24例年龄相仿的正常晚期妊娠胎盘组织(对照组)中HLA-G的表达差异。结果 子痫前期患者胎盘组织中HLA-G基因与蛋白表达水平均显著低于正常妊娠组，两者比较差异有统计学意义(P<0.01)，轻度与重度子痫前期比较差异无统计学意义(P>0.05)。结论 子痫前期胎盘组织HLA-G的表达明显下降，这一异常可能参与了子痫前期的发生和病理生理过程，但不能作为病情严重程度的筛选指标。
Abstract:
Objective To investigate the role of HLA-G in pre-eclampsia(PE) by examination of the expression of HLA-G in placentae from PE and normal pregnant. Methods The expression of HLA-G was detected in 24 cases of PE patients (experimental group, with 12 cases of mild PE and 12 cases of severe PE) and 24 cases of normal placentae in their late pregnancy (control group) using RT-PCR and Western Blotting respectively. Results Compared with control group, the levels of mRNA and protein of HLA-G in the placentae from patients with preeclampsia were significantly low (P<0.01).There was no significance in expression of HLA-G between mild and severe PE patients (P>0.05). Conclusion The expression level of HLA-G in placentae of PE patients decreased significantly. The abnormal expression of HLA-G might connect with the occurrence and pathology & physiology processes of PE, but can not used as a scanning factor for evaluation of the severity of the PE.

参考文献/References:

[1] 乐 杰. 妇产科学[M].6 版. 北京:人民卫生出版社，2004:105.
[2] Clements CS,Kjer-Nielsen L,Kostenko L,et al. Crystal structure of HLA-G:a nonclassical MHC class I molecule expressed at the fetal-matemal interface [J]. Proc Natl Acad Sci, 2005,102(9):3360-3365.
[3] Hviid TV.Non-classical human leukocyte antigen (HLA) tissue types-from implantation to trasplantation [J]. Ugeskr Laeger,2006,168 (5) :461-466.
[4] Zhang L, Wang Y, Liao AH. Quantitative abnormalities of fetal trophoblast cells in maternal circulation in preeclampsia[J]. Prenat Diagn,2008, 28(12):1160-1166.
[5] Iversen AC, Nyugen OT, Tommerdal LF, et al. The HLA-G 14 bp gene polymorphism and decidual HLA-G 14bp gene expression in pre-eclamptic and normal pregnancies[J]. J Reprod Immunol, 2008,78 (2) :158-165.
[6] Hara N, Fujii T, Yamashita T. Altered expression of human leukocyte antigen G (HLA-G) on extravillous trophoblasts in preeclampsia:immunohistological demonstration with anti-HLA-G specific antibody “87G” and anti-cytokeratin antibody “CAM5.2”[J]. Am J Reprod Immunol,1996, 36(6): 349-358.
[7] Goldman-Wohl DS, Ariel I, Greenfield C, et al. Lack of human leukocyte antigen-G expression in extravillous trophoblasts is associated with pre-eclampsia[J]. Mol Hum Reprod, 2000, 6(1): 88-95.
[8] Yie SM, Li LH, Li YM, et al. HLA-G protein concentrations in maternal serum and placental tissue are decreased in preeclampsia[J]. Am J Obstet Gynecol,2004, 91(2):525-529.
[9] Kanai T, Fujii T, Unno N, et al. Human leukocyte antigen-G-expressing cells differently modulate the release of cytokines from mononuclear cells present in the decidua versus peripheral blood[J]. Am J Reprod Immunol,2001,45(2):94-99.
[10]Tan CY, Ho JF, Chong YS, et al. Paternal contribution of HLA-G 0106 significantly increases risk for pre-eclampsia in multigravid pregnancies[J]. Mol Hum Reprod, 2008,14(5):317-324
[11]Sageshima N, Ishitani A, Omura M, et al. Necrotic feature of the trophoblasts lacking HLA-G expression in normal and pre-eclamptic placentas[J]. Am J Reprod Immunol,2003,49(3):174-182.
[12]Datema G, Van Meir CA, Kanhai HH, et al. Pre-term birth and severe pre-eclampsia are not associated with altered expression of HLA on human trophoblasts[J]. Am J Reprod Immunol,2003,49(4): 193-201.

相似文献/References:

[1]李琪虹,周敬勉.妊娠期糖尿病并发子痫前期对脐动脉血流的影响[J].医学研究与战创伤救治(原医学研究生学报),2016,18(01):41.[doi:10.3969/j.issn.1672-271X.2016.01.012]
　LI Qi-hong,ZHOU Jing-mian..The influences of gestational diabetes complicating preeclampsiaon umbilical artery[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2016,18(01):41.[doi:10.3969/j.issn.1672-271X.2016.01.012]

备注/Memo

备注/Memo:
-
更新日期/Last Update: 2010-01-20