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[1]万 涛,周宁新,徐东刚,等.胆管癌相关FXYD6基因功能区在大肠埃希菌表达的初步研究[J].医学研究与战创伤救治(原医学研究生学报),2010,12(04):289-292.
　WAN Tao,ZHOU Ning-xin,XU Dong-gang,et al.Prokaryotic expression of functional domain of a novel FXYD6 gene related to cholangiocarcinoma[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2010,12(04):289-292.
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胆管癌相关FXYD6基因功能区在大肠埃希菌表达的初步研究()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第12卷
期数:
2010年04期
页码:
289-292
栏目:
出版日期:
2010-07-20

文章信息/Info

Title:
Prokaryotic expression of functional domain of a novel FXYD6 gene related to cholangiocarcinoma
文章编号:
1672-271X(2010)04-0289-04
作者:
万 涛1; 周宁新2; 徐东刚3; 吴加金3; 黄志强1
1.100853 北京，解放军总医院肝胆外科研究所；2.100088 北京，第二炮兵总医院肝胆胃肠病研究所；3.100850 北京，解放军军事医学科学院基础医学研究所
Author(s):
WAN Tao1; ZHOU Ning-xin2; XU Dong-gang3; WU Jia-jin3; HUANG Zhi-qiang1
1.Institute of Hepatobiliary Surgery of General Hospital of PLA，Beijing 100853, China;2.Institute of Hepatobiliary and Gastroenteric Diseases of the Second Artillery General Hospital of PLA，Beijing 100088, China;3.Institute of Basic Medicine, Academy of M
关键词:
胆管癌; 离子通道蛋白; FXYD6基因; 功能区; 表达
Keywords:
cholangiocarcinoma;  ion channel; FXYD6 gene;  cloning;  functional domains
分类号:
R575.7;R735.8
DOI:
-
文献标志码:
A
摘要:
目的 对胆管癌相关基因FXYD6基因的功能区进行克隆和表达，以便进一步展开该基因功能研究。方法 借助生物信息学方法分析FXYD6基因，设计FXYD6基因信号肽剪切后功能区蛋白体外表达序列，并将其克隆到pBV220表达载体上，继而以大肠埃希菌为表达系统，使功能区蛋白(不含信号肽)获得体外表达。结果 该基因主要功能位点位于18～95 aa。PCR扩增出剪切掉信号肽序列的功能区序列(即包含18～95 aa的肽链段)，并成功构建pBV220/FXYD6－ex基因功能区重组表达质粒克隆，其在大肠埃希菌DH5α中表达量最高，表达产物主要以包涵体形式存在。 结论 获得胆管癌相关FXYD6基因融合蛋白表达产物，为后续研究打下基础。
Abstract:
Objective To obtain the clone and expression of the functional domain of FXYD6 gene, which is a novel cholangiocarcinoma related gene. Methods Bioinformatical methods were used to analyze the full-length sequence, and predict the functional domains of its protein. The sequence of functional domain without signal peptide was designed and obtained by polymerase chain reaction, then the recombinant expression plasmid pBV220/FXYD6-ex was constructed to express human FXYD6 functional domain in the host bacteria E.coli. Results The functional domains were located from 18 aa to 95 aa，and the corresponding objective sequence(FXYD6-ex) was obtained by polymerase chain reaction. The recombinant expression plasmid pBV220/FXYD6-ex was constructed to express human FXYD6 functional domain in the host bacteria. The highest expression level of the target protein was in E.coli DH5α. Most of the target protein was in the inclusion bodies. Conclusion This study provides the basis for understanding the relationship between the structure and function of FXYD6. More work should be performed on the novel protein and its influence on the mechanism of cholangiocarcinoma.

参考文献/References:

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备注/Memo

备注/Memo:
国家自然科学基金面上项目(30471699)
更新日期/Last Update: 2010-07-20