|本期目录/Table of Contents|

[1]方 健,宋海燕,陈从新,等.一起小规模群体肺炎链球菌感染流行的报告[J].医学研究与战创伤救治(原医学研究生学报),2011,13(06):485-487.
　FANG Jian,SONG Hai-yan,CHEN Cong-xin,et al.The prevalence of streptococcus pneumoniae among a military camp[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2011,13(06):485-487.
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一起小规模群体肺炎链球菌感染流行的报告()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第13卷
期数:
2011年06期
页码:
485-487
栏目:
出版日期:
2011-11-20

文章信息/Info

Title:
The prevalence of streptococcus pneumoniae among a military camp
文章编号:
1672-271X(2011)06-0485-03
作者:
方 健1; 宋海燕1; 陈从新1; 刘 波1; 张骏飞1; 吴伟立2
1.230031 安徽合肥，解放军105医院；2.101300 北京，中国科学院北京基因研究所
Author(s):
FANG Jian1; SONG Hai-yan1; CHEN Cong-xin1; LIU Bo1; ZHANG Jun-fei1; WU Wei-li2
1.105 Hospital of PLA,Hefei,Anhui 230031,China;2.Beijing Gene Institute,Beijing 101300,China
关键词:
发热; 聚合酶链反应; 肺炎链球菌
Keywords:
fever;  polymerase chain reaction;  streptococcus pneumoniae
分类号:
R37
DOI:
-
文献标志码:
A
摘要:
目的 明确一次小规模不明原因发热疫情的病因。方法 常规提取6例发热患者和11例密切接触者咽拭子DNA和RNA，采用荧光定量PCR法检测15种病原体，取扩增产物进行测序鉴定，并与常规培养结果比对；采用荚膜肿胀试验检测肺炎链球菌血清型。结果 在17例咽拭子中培养出肺炎链球菌阳性11例(64.7%)，血清型鉴定为6群6A型，未分离到其他病原菌；在17例咽拭子标本中，检出13例(76.5%)肺炎链球菌DNA阳性，2例(11.8%)嗜肺军团菌DNA阳性，3例(17.6%)肺炎支原体阳性，1例(5.9%)乙型流感病毒RNA阳性；在其中5例中，3例肺炎链球菌与肺炎支原体同时阳性，1例肺炎链球菌与乙型流感病毒同时阳性，另1例密切接触者同时检出肺炎链球菌与嗜肺军团菌；对所有CT值小于35的咽拭子DNA经普通PCR扩增后测序，并经NCBI基因库比对，结果与相应病原体的基因组序列相符。结论 肺炎链球菌为引起此次疫情流行的主要病因。
Abstract:
Objective To investigate the etiological causes of fever with unknown origin in soldiers in a military camp. Methods The DNA and RNA from throat swabs in six soldiers with fever of unknown origin and eleven close contacts without fever were detected by real-time fluorescent quantitative PCR assay for fifteen species of common respiratory pathogens and the serotypes of streptococcus pneumoniae were determined by Quel-lung test. Results The positive of streptococcus pneumoniae detected by swab culture was 64.7%(11/17) and the predominant strain was serotype 6A. Out of the 17 swabs,the streptococcus pneumoniae was positive in 13 (76.5%) by PCR. Legionella pneumophila was in 2 (11.8%),mycoplasma pneumoniae in 3 (17.6%) and influenze B virus in 1(5.9%). All specimens were positive for streptococcus pneumoniae detected by real-time fluorescent quantitative PCR and were confirmed by sequencing. Conclusion Streptococcus pneumoniae is the pathogen for this upper-respiratory infection in a military camp. The real-time PCR assay used in this study is sensitive and specific for detecting common pathogens in nasopharyngeal swabs.

参考文献/References:

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备注/Memo

备注/Memo:
全军“十一五”科技攻关项目(2008G021)
更新日期/Last Update: 2011-11-20