|本期目录/Table of Contents|

[1]王 璇,时姗姗,马恒辉,等.非小细胞肺癌表皮生长因子受体基因突变的结果分析[J].医学研究与战创伤救治(原医学研究生学报),2012,14(05):387-389.
　WANG Xuan,SHI Shan-shan,MA Heng-hui,et al.Respective analysis of EGFR mutations in non-small cell lung cancer[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2012,14(05):387-389.
点击复制

非小细胞肺癌表皮生长因子受体基因突变的结果分析()
分享到62.8K

《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第14卷
期数:
2012年05期
页码:
387-389
栏目:
出版日期:
2012-09-20

文章信息/Info

Title:
Respective analysis of EGFR mutations in non-small cell lung cancer
文章编号:
1672-271X(2012)05-0387-03
作者:
王 璇; 时姗姗; 马恒辉; 周晓军; 王建东
210002 江苏南京，南京军区南京总医院病理科
Author(s):
WANG Xuan; SHI Shan-shan; MA Heng-hui; ZHOU Xiao-jun; WANG Jian-dong
Department of Pathology,Nanjing General Hospital of Nanjing Military Command,PLA,Nanjing，Jiangsu 210002,China
关键词:
肺癌; 表皮生长因子受体; 突变
Keywords:
lung cancer;  EGFR;  mutation
分类号:
R574
DOI:
-
文献标志码:
A
摘要:
目的 回顾性分析301例非小细胞肺癌(NSCLC)组织表皮生长因子受体(EGFR)基因突变检测结果，比较三种实验方法检查EGFR突变的差异。探讨肺癌临床靶向个体化治疗进行EGFR分子病理检查的最佳方法。方法 应用聚合酶链反应(PCR)结合直接测序法检测171例肺癌DNA样本；TaqMan探针荧光定量PCR法检测88例肺癌DNA样本；扩增阻碍突变系统(ARMS)法检测42例肺癌DNA样本。结果 PCR直接测序法、TaqMan探针荧光定量PCR法、ARMS法的阳性总检出率分别是31.58%、27.27%、42.86%。结论 PCR直接测序法和TaqMan探针荧光定量PCR法阳性总检出率差异无统计学意义(P>0.05)，ARMS法阳性总检出率高于PCR直接测序法和TaqMan探针荧光定量PCR法(P均＜0.01)。对于不同来源的肺癌组织标本，不同方法检测EGFR突变具有各自的优点和不足。
Abstract:
Objective The results of epidermal growth factor receptor (EGFR) mutations in 301 tissues with non-small cell lung cancer (NSCLC) detected by three different methods were respectively analyzed.The differences among three methods were compared.The objective of this study was to explore the proper molecular method for personalized target therapy in lung cancer.Methods Polymerase chain reaction combine with direction sequencing was used to detect EGFR mutations in 171 patients.Real-time PCR with TaqMan fluorecent probe was used to detect EGFR mutations in 88 patients and amplification refractory mutation system PCR (ARMS-PCR) was used to detect EGFR mutations in 42 patients.Results The total positive rates for sequencing,TaqMan real-time PCR,and ARMS-PCR methods were 31.58%,27.27%,and 42.86% respectively.Conclusion No significant difference in total positive rate between direct sequencing and TaqMan real-time PCR (P>0.05).The total positive rate was higher in ARMS-PCR than that in direct sequencing and TaqMan real-time PCR (P<0.01).To different tissues,different methods have their superiority when used for detection of EGFR mutations in lung cancer.

参考文献/References:

[1]周诚忠，夏炎春，夏海波，等.三维适形放射治疗老年非小细胞肺癌60例[J].东南国防医药,2009,11(1):20-21.
[2]王晓萍，丰俊东.放射治疗与分子靶向药物在肿瘤治疗中的联合应用[J].东南国防医药,2008,10(4):277-280.
[3]Dahabreh IJ,Linardou H,Siannis F,et al.Somatic EGFR mutation and gene copy gain as predictive biomarkers for response to tyrosine kinase inhibitors in non-small cell lung cancer[J].Clin Cancer Res,2010,16(1):291-303.
[4]Sholl LM,Xiao Y,Joshi V,et al.EGFR mutation is a better predictor of response to tyrosine kinase inhibitors in non-small cell lung carcinoma than FISH,CISH,and immunohistochemistry[J].Am J Clin Pathol,2010,133(6):922-934.
[5]宋海珠，易 俊，黄桂春，等.人肺腺癌细胞基因表型与尼妥珠单抗疗效的关系[J].医学研究生学报,2011,24(2):148-153.
[6]Penzel R,Sers C,Chen Y,et al.EGFR mutation detection in NSCLC——assessment of diagnostic application and recommendations of the german panel for mutation testing in NSCLC[J].Virchows Arch,2011,458(1):95-98.
[7]Han HS,Lim SN,An JY,et al.Detection of EGFR mutation status in lung adenocarcinoma specimens with different proportions of tumor cells using two methods of differential sensitivity[J].J Thorac Oncol,2012,7(2):355-364.
[8]张 静，高 洁，梁智勇，等.非小细胞肺癌表皮生长因子受体基因突变检测的经验总结分析[J].中华病理学杂志,2011,40(10):712-714.
[9]王 芳，付 莎，汤 涛，等.非小细胞肺癌表皮生长因子受体基因突变与临床病理特征的关系[J].中华病理学杂志,2011,40(10):664-666.
[10]Cheng H,Xu X,Costa DB,et al.Molecular testing in lung cancer:the time is now[J].Curr Oncol Rep,2010,12(5):335-348.
[11]王立帅，张 瑜，陆小军，等.双环探针特异引物荧光聚合酶链反应法检测非小细胞肺癌表皮生长因子受体基因突变[J].中华病理学杂志,2011,40(10):667-670.
[12]Bardelli A,Janne PA.The road to resistance:EGFR mutation and cetuximab[J].Nat Med,2012,18(2):199-200.

相似文献/References:

[1]许德兵,胡 燕,王 颖,等.女性肺癌95例临床分析[J].医学研究与战创伤救治(原医学研究生学报),2013,15(03):255.[doi:10.3969/j.issn.1672-271X.2013.03.016]
　XU De-bing,HU Yan,WANG Ying,et al.Clinical analysis of 95 cases of female lung cancer[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2013,15(05):255.[doi:10.3969/j.issn.1672-271X.2013.03.016]
[2]时维东,陶小红,丁汇清,等.女性肺癌的影像诊断与临床对照分析[J].医学研究与战创伤救治(原医学研究生学报),2013,15(01):35.
　SHI Wei-dong,TAO Xiao-hong,DING Hui-qing,et al.Imaging diagnosis and clinical comparative study of lung cancer in female[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2013,15(05):35.
[3]郑大东,钟 勇.肿瘤标志物及PET未见异常的肺癌1例[J].医学研究与战创伤救治(原医学研究生学报),2012,14(01):70.
[4]周 源,汪 栋,张传生,等.肺癌患者术前病理诊断的临床意义[J].医学研究与战创伤救治(原医学研究生学报),2008,10(02):97.
　ZHOU Yuan,WANG Dong,ZHANG Chuan-sheng,et al.Clinical significance of preoperatively pathological diagnosis of lung cancer[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2008,10(05):97.
[5]许德兵,潘士勇,史兆荣,等.肺癌合并胸腔积液172例临床分析[J].医学研究与战创伤救治(原医学研究生学报),2008,10(02):107.
　XU De-bing,PAN Shi-yong,SHI Zhao-rong,et al.Clinical analysis of 172 cases of malignant pleural effusion with lung cancer[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2008,10(05):107.
[6]庄聪文,翁向群,程先进,等.胺碘酮转复肺癌术后室上性心律失常的 疗效分析[J].医学研究与战创伤救治(原医学研究生学报),2008,10(04):252.
　ZHUANG Cong-wen,WENG Xiang-qun,CHENG Xian-jin,et al.Analysis of amiodarone therapy for supraventricular arrhythmias after operation in patients with lung cancer[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2008,10(05):252.
[7]许德兵,吴凌云综述,宋 勇审校.miRNA与肺癌关系的研究进展[J].医学研究与战创伤救治(原医学研究生学报),2014,16(01):73.
[8]艾书跃,杨凤娇,袁 梅,等.原发性肺癌原发灶PET/CT征象与发生淋巴结及远处转移的相关性研究[J].医学研究与战创伤救治(原医学研究生学报),2015,17(02):138.[doi:10.3969/j.issn.1672-271X.2015.02.008]
　AI Shu-yue,YANG Feng-jiao,YUAN Mei,et al.Correlation between the PET/CT signs of primary lung cancer and metastases[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2015,17(05):138.[doi:10.3969/j.issn.1672-271X.2015.02.008]
[9]肖鑫武,孙文逵,刘亚芳,等.超声支气管镜引导下的经支气管镜针吸活检术在纵隔及肺部病变诊疗中的应用价值[J].医学研究与战创伤救治(原医学研究生学报),2015,17(03):239.[doi:10.3969/j.issn.1672-271X.2015.03.005]
　XIAO Xin-wu,SUN Wen-kui,LIU Ya-fang,et al.The application value of endobronchial ultrasound-guided transbronchial needle aspiration in diagnosis and treatment of mediastinal and pulmonary diseases[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2015,17(05):239.[doi:10.3969/j.issn.1672-271X.2015.03.005]
[10]禹乐综述,余英豪审校.非小细胞肺癌胸腔积液EGFR突变检测方法的研究进展[J].医学研究与战创伤救治(原医学研究生学报),2017,19(04):380.[doi:10.3969/j.issn.1672-271X.2017.04.012]

备注/Memo

备注/Memo:
国家自然科学基金(30970813；81171391)
更新日期/Last Update: 2012-09-20