|本期目录/Table of Contents|

[1]陈 琼,郑锦峰,孙军红,等.量子点荧光探针用于汉坦病毒重组抗原的检测[J].医学研究与战创伤救治(原医学研究生学报),2012,14(06):483-486.
　CHEN Qiong,ZHENG Jin-feng,SUN Jun-hong,et al.Detection of recombinant Hantavirus antigens by using water-solubility quantum dots fluorescent probe[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2012,14(06):483-486.
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量子点荧光探针用于汉坦病毒重组抗原的检测()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第14卷
期数:
2012年06期
页码:
483-486
栏目:
出版日期:
2012-11-20

文章信息/Info

Title:
Detection of recombinant Hantavirus antigens by using water-solubility quantum dots fluorescent probe
文章编号:
1672-271X(2012)06-0483-04
作者:
陈 琼1; 5; 郑锦峰2; 孙军红3; 姚苹苹4; 王 洁1; 李 晶5; 邓小昭5; 王长军5; 朱函坪4; 张 云1; 5
1.210029 江苏南京，南京医科大学公共卫生学院；2.210002 江苏南京，南京军区南京总医院营养科；3.210016 江苏南京，南京军区联勤部卫生部；4.310051 浙江杭州，浙江省疾病预防控制中心；5.210002 江苏南京，南京军区疾病预防控制中心
Author(s):
CHEN Qiong1; 5; ZHENG Jin-feng2; SUN Jun-hong3; YAO Ping-ping4; WANG Jie1; LI Jing5; DENG Xiao-zhao5; WANG Chang-jun5; ZHU Han-ping4; ZHANG Yun1; 5
1.School of Public Health，Nanjing Medical University，Nanjing,Jiangsu 210029，China;2.Nanjing General Hospital of Nanjing Military Command,Nanjing,Jiangsu 210002,China;3.Division of Health,Department of Joint Logistics,Nanjing Military Command，Nanjing,Jiangsu 210002,China;4.Zhejiang Center of Disease and prevention,Hangzhou,Zhejiang 310051,China;5.Medical Insticute of Nanjing Military Command，Nanjing,Jiangsu 210002,China
关键词:
量子点荧光探针; 汉坦病毒; 重组抗原
Keywords:
quantum dots fluorescent probe;  Hantavirus;  recombinant antigens
分类号:
R446.621
DOI:
-
文献标志码:
A
摘要:
目的 应用量子点荧光探针对汉坦病毒(Hantavirus，HV)重组抗原进行检测。 方法 合成水溶性量子点荧光纳米颗粒，并在其表面修饰G蛋白和anti-HV抗体作为量子点荧光探针，对HV重组抗原进行检测并优化检测条件。结果 量子点与抗体的最佳偶联条件:pH 6.0、反应时间2 h、抗体浓度为20 μg/ml。用本方法检测HV重组抗原的最低检测值为5 ng/ml。 结论 该探针能有效的识别HV抗原，且操作简便快速，为HV重组抗原的检测和肾出血热综合征的诊断提供了新方法。
Abstract:
Objective To detect recombinant Hantavirus antigens by using a quantum dots (QDs) fluorescent probe.Methods The probe of water-solubility QDs fluorescence nanoparticle was synthesized and modified with protein G and anti-HV Antibodies.We detected recombinant Hantavirus antigens by probe combining immune method，and optimized the detecting conditions.Results The optimum reaction time,pH and goat antibodies concentration for conjugating the QDs were 2 h,6.0,and 20 μg/ml,respectively.The limit of detection of recombinant antigens was 5 ng/ml.Conclusion The fluorescent probe could effectively recognize HV antigens.The method was specific，sensitive and convenient.We developed a new method for HV antigens detection.

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备注/Memo

备注/Memo:
国家自然科学基金(81171609)；浙江省自然科学基金(Y205749)
更新日期/Last Update: 2012-11-20