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[1]孙国静,杨书丰,郭国栋,等.重组成骨蛋白-1在钛合金微粒环境下对MC3T3-E1成骨细胞增殖、分化、矿化的影响[J].医学研究与战创伤救治(原医学研究生学报),2015,17(01):1-4.[doi:10.3969/j.issn.1672-271X.2015.01.001]
　UN Guo-jing,YANG Shu-feng,GUO Guo-dong,et al.Effect of rop-1 on the proliferation， diferentiation， and mineralizatio of titanium alloy particles-induced MC3T3-E1 osteoblastic cells[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2015,17(01):1-4.[doi:10.3969/j.issn.1672-271X.2015.01.001]
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重组成骨蛋白-1在钛合金微粒环境下对MC3T3-E1成骨细胞增殖、分化、矿化的影响()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第17卷
期数:
2015年01期
页码:
1-4
栏目:
出版日期:
2015-01-31

文章信息/Info

Title:
Effect of rop-1 on the proliferation， diferentiation， and mineralizatio of titanium alloy particles-induced MC3T3-E1 osteoblastic cells
作者:
孙国静1; 杨书丰2; 郭国栋1; 樊根涛1; 吴 俊1; 赵建宁1
1.210002 江苏南京，南京军区南京总医院骨科；2.210002 江苏南京，解放军81医院骨科
Author(s):
UN Guo-jing1;  YANG Shu-feng2;  GUO Guo-dong1;  FAN Gen-tao1;  WU Jun1;  ZHAO Jian-ning1.
1．Department of Orthopedics， Nanjing General Hospital of Nanjing Military Command， Nanjing， Jiangsu 210002， China； 2．Department of Orthopedics， 81 Hospital of PLA， Nanjing， Jiangsu 210002， China
关键词:
钛合金微粒; 重组成骨蛋白-1; 成骨细胞; 骨溶解
Keywords:
titanium alloy particles;  recombinant OP-1;  osteoblast;  osteolysis
分类号:
R684.3
DOI:
10.3969/j.issn.1672-271X.2015.01.001
文献标志码:
A
摘要:
目的 在钛-6铝-4钒(Ti-6Al-4V)合金微粒环境下观察重组成骨蛋白-1(recombinant OP-1，rOP-1)对成骨细胞的影响，为防治关节假体无菌性松动提供新的治疗途径。方法 根据小鼠颅顶骨前成骨细胞亚克隆14(MC3T3-E1)中是否加入Ti-6Al-4V微粒和rOP-1，分为微粒组(5、10、15 μg/mL Ti-6Al-4V)、处理组(微粒组加入200 ng/mL rOP-1)、阳性组(加入200 ng/mL rOP-1)和对照组，检测各组24、72、120 h MC3T3-E1细胞增殖能力、72 h碱性磷酸酶(akaline phosphatase，AKP)、骨钙素(osteocalcin，OCN)和骨桥蛋白(osteopontin，OPN)mRNA的表达，及120 h成骨细胞的矿化能力。结果 ①rOP-1无促进Ti-6Al-4V微粒环境下成骨细胞增殖能力，与微粒组比较，差异无统计学意义(P>0.05)；②rOP-1可提高成骨细胞分化，与对照组比较差异有统计学意义(P＜0.05)；同时逆转Ti-6Al-4V微粒抑制成骨细胞分化，与微粒组比较差异有统计学意义(P＜0.05)；③茜素红S染色后Ti-6Al-4V微粒钙结节数量随着浓度增加逐渐降低，和微粒组比较，加入rOP-1后钙结节数量呈增多趋势。结论 Ti-6Al-4V微粒环境下，rOP-1无提高成骨细胞增殖能力，能提高细胞分化矿化能力，rOP-1可以作为潜在治疗关节假体无菌性松动一种方法。
Abstract:
Objective Our results has been shown that titanium alloy particles (Ti-6Al-4V) inhibited RUNX2 mRNA andproteinexpression of osteoblasts. In order to improve the boneformation on osteoblasts induced-Ti-6Al-4V， we observed the effect of rOP-1 on the proliferation， diferentiation， andmineralizatio of Ti-6Al-4V induce osteoblast， to provide a new treatment method of artificial joint aseptic loosening. Methods Cell counting Kit8 test used to assay the cell proliferation after 24 h， 72 h and 120 h-incubation with Ti-6Al-4V±rOP-1， respectively. Furthermore， alkaline phosphatase (AKP)， osteocalcin (OCN) and osteopontin (OPN) mRNA level were detected by reverse transcription-polymerase chain reaction (RT-PCR)， respectively， at the time point of 72 h. Cell bio-mineralization was assessed through alizarin red S staining after 120 h. Results ①Compared with Ti-6Al-4V group， after 24 h， 72 h and 120 h-incubation with Ti-6Al-4V+rOP-1， the proliferation of osteoblast did not increase significance with comparison of the Ti-6Al-4V group (P>0.05)； ②compared with Ti-6Al-4V group， AKP， OCN and OPN mRNA level were increased by 72 h-exposure to Ti-6Al-4V+rOP-1， respectively (P<0.05). rOP-1can stimulate osteoblast differentiation and mineralization， compared with Ti-6Al-4V group， there was statistically significance (P<0.05)； ③compared with Ti-6Al-4V group， alizarin red staining demonstrated that the quantity of calcium nodules were markedly increased in the Ti-6Al-4V+rOP-1 group at hours 120. Conclusion Our result indicate that rOP-1 can stimulate differentiation and mineralization， while the proliferation remained unchanged on osteoblast with Ti-6Al-4V particles. We suppose that rOP-1 may be a therapeutic candidate for the prevention of artificial joint aseptic loosening.

参考文献/References:

［1］孙国静，杨书丰，郭 亭，等.不同浓度钛合金微粒对成骨细胞信号通路中转录表达因子RUNX2影响［J］．医学研究生学报，2013，26(3):251-254.
［2］Takahashi T，Muneta T，Tsuji K，et al．BMP-7 inhibits cartilage degeneration through suppression of inflammation in rat zymosan-induced arthritis［J］．Cell Tissue Res，2011，344(2):321-332．
［3］Barr T，McNamara AJ，Sándor GK，et al．Comparison of the osteoinductivity of bioimplants containing recombinant human bone morphogenetic proteins 2 (Infuse) and 7 (OP-1)［J］．Oral Surg Oral Med Oral Pathol Oral Radiol Endod，2010，109(4):531-540.
［4］Spiro AS，Beil FT，Baranowsky A，et al.BMP-7-induced ectopic bone formation and fracture healing is impaired by systemic NSAID application in C57BL/6-mice［J］．J Orthop Res，2010，28(6):785-791.
［5］陶伟伟，朱建非，张 穹，等.股骨头无菌性坏死中高保留骨量技术应用在初次人工全髋关节置换的临床疗效［J］．东南国防医药，2014，16(2):135-137.
［6］Purdue PE，Koulouvaris P，Potter HG，et al．The cellular and molecular biology of periprostheticosteolysis［J］．Clin Orthop Relat Res，2007，454:251-261.
［7］Zhang F，Ren LF，Lin HS，et al．The optimal dose of recombinant human osteogenic protein-1 enhances differentiation of mouse osteoblast-like cells:an in vitro study［J］．Arch Oral Biol，2012，57(5):460-468.
［8］Goodman SB，Ma T，Chiu R，et al．Effects of orthopaedic wear particles on osteoprogenitor cells ［J］．Biomaterials，2006，27(36):6096-6101.
［9］Jiang Y，Jia T，Gong W，et al．Effects of Ti，PMMA，UHMWPE，and Co-Cr wear particles on differentiation and functions of bone marrow stromal cells［J］．J Biomed Mater Res A，2013，101(10):2817-2825．
［10］Shawn K，Richard C，Ting Ma，et al.OP-1 (BMP-7) stimulates osteoprogenitor cell differentiation in thepresence of polymethylmethacrylate particles［J］．J Biomed Mater Res A，2010，94(2):485-488．
［11］Chiu R，Ma T，Smith RL，et al．Polymethylmethacrylate particles inhibit osteoblastic differentiation of MC3T3-E1 osteoprogenitor cells［J］．J Orthop Res，2008，26(7):932-936．
［12］Kann S，Chiu R，Ma T，Goodman SB.OP-1(BMP-7)stimulatesosteoprogenitorcell differentiation in the presence of polymethylmethacrylate particles［J］．J Biomed Mater Res A，2010，94(2):485-488.

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备注/Memo

备注/Memo:
江苏省临床医学科技专项(BL2012002)；南京军区医学科技创新项目(11Z028)；南京市科技发展项目(2012sc311020)
更新日期/Last Update: 2015-01-20