|本期目录/Table of Contents|

[1]于 淼,原 源.HPLC法测定益心酮片中四种黄酮类成分的含量[J].医学研究与战创伤救治(原医学研究生学报),2015,17(02):157-159，165.[doi:10.3969/j.issn.1672-271X.2015.02.014]
　YU Miao,YUAN Yuan..Determination of four flavonoid ingredients in Yixintong tablets by HPLC[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2015,17(02):157-159，165.[doi:10.3969/j.issn.1672-271X.2015.02.014]
点击复制

HPLC法测定益心酮片中四种黄酮类成分的含量()
分享到62.8K

《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第17卷
期数:
2015年02期
页码:
157-159，165
栏目:
出版日期:
2015-04-22

文章信息/Info

Title:
Determination of four flavonoid ingredients in Yixintong tablets by HPLC
作者:
于 淼; 原 源
200052 上海，解放军85医院药剂科
Author(s):
YU Miao;  YUAN Yuan.
Department of Pharmacy， 85 Hospital of PLA， Shanghai 200433， China
关键词:
高效液相色谱法; 益心酮片; 牡荆素鼠李糖苷; 牡荆素葡萄糖苷; 牡荆素; 金丝桃苷
Keywords:
HPLC;  Yixintong tablets;  vitexin rhamnosides;  vitexin glucoside;  vitexin;  hyperoside
分类号:
R286
DOI:
10.3969/j.issn.1672-271X.2015.02.014
文献标志码:
A
摘要:
目的 建立高效液相色谱法（HPLC）同时测定益心酮片中牡荆素鼠李糖苷、牡荆素葡萄糖苷、牡荆素、金丝桃苷四种成分的含量。方法 采用SHISEIDO CAPCELL Pak C18 AQ 250 mm×4.6 mm色谱柱；流动相为四氢呋喃-乙酸-水-甲醇-乙腈(38∶4∶152∶3∶3)；流速为1．0 mL／min；柱温：25 ℃；检测波长：330 nm。结果 牡荆素鼠李糖苷、牡荆素葡萄糖苷、牡荆素、金丝桃苷的线性范围分别为10.11~201.02 μg/mL(r=0.9997)，7.92~158.42 μg/mL(r=0.9996)，4.02~80.42 μg/mL(r=0.9995)，6.28~125.61 μg/mL(r=0.9995)，范围内线性关系良好、精密度、重现性、回收率均符合要求。结论 该方法快速、准确、重现性好可用于本品的质量控制。
Abstract:
Objective To establish the quantitative analysis method for simultaneous determination of Vitexin rhamnosides， Vitexin glucoside， Vitexin and Hyperoside in Yixintong tablets by HPLC. Methods HPLC was performed on SHISEIDO CAPCELL Pak C18 AQ (4.6 mm×250 mm)， with the mobile phase of THF-acetic acid-water-methanol-acetonitrile (38∶4∶152∶3∶3) at a flow rate of 1.0 mL/min．The column temperature was set at 25 ℃， and the detection wavelength was 330 nm. Results The linear ranges of Vitexin rhamnoside， Vitexin glucoside， Vitexin， Hyperoside were 10.11-201.02 μg/mL (r=09997)， 7.92-158.42 μg/mL (r=0.9996)， 4.02-80.42 μg/mL (r=0.9995)， 6.28-125.61 μg/mL (r=0.9995) respectively. The linear relationship， precision， reproducibility， recovery rates all meet the requirements. Conclusion The content of Vitexin rhamnosides is significantly higher than Hyperoside， and the study shows that Vitexin rhamnosides has effect on dilating blood vessels， protecting cardiac tissue， which is consistent with the effect of the Yixintong tablets. Therefore， it's not reasonable to choose Hyperoside as the only quality control indicator of the Yixintong tablets in the 2010 Chinese Pharmacopoeia. Vitexin rhamnoside and Vitexin glucoside are also important pharmacological active components in Hawthorn Leaves， and could be considered as quality control indicators of the Yixintong tablets.

参考文献/References:

［1］宋少江，陈 佳，寇 翔，等．山楂叶的化学成分［J］．沈阳药科大学学报，2006，23(2)：88-90．
［2］丁杏苞，姜碉青．山楂叶化学成分的研究［J］．中国中药杂志，1990，15(5)：39-41．
［3］杨利平，王春霖，王永利，等．山楂叶提取物对家兔血小板聚集和大鼠实验性心肌缺血的影响［J］．中草药，1993，24(9)：482-483．
［4］刘全亮，杨中林．不同纯度山楂叶总黄酮降血脂作用的比较研究［J］．海峡药学，2008，20(2)：23-24．
［5］闵 清，白育庭，舒思洁，等．山楂叶总黄酮对大鼠心肌缺血再灌注损伤的保护作用［J］．中药药理与临床，2005，21(2)：19-21.
［6］Fürst R，Zirrgiebel U，Totzke F，et al．The Crataegus extract WS 1442 inhibits balloon catheter-induced intimal hyperplasia in the rat carotid artery by directly influencing PDGFR-beta［J］．Atherosclerosis，2010，211(2)：409-417．
［7］Chen J，Song SJ，Song N.Simultaneous determination of 2"-0-rhamnosyl vitexin and vitexin in Chinese hawthorn leaf and its extract by RP-HPLC［J］.J Chin Pharm Sci，2006，15(1)：51-54.
［8］马 国，蒋学华，黄 婷，等.HPLC同时测定山楂叶提取物中的5种主要成分［J］.华西药学杂志，2007，22（5）：547-549.
［9］中国药典2010版一部［S］.2010：1026.
［10］王福成，豆文太，朱文学．HPLC测定山楂精中牡荆苷、牡荆苷-2"-0-鼠李糖苷、金丝桃苷、芦丁的含量［J］．中成药，2002，24(2)：122-124.
［11］朱晓新，李连达，刘建勋，等．牡荆素鼠李糖苷对脐静脉内皮细胞缺氧再给氧损伤的影响［J］．中国实验方剂学杂志，2005，11(6)：30-33.
［12］朱晓新，李连达，刘建勋，等．牡荆素鼠李糖苷对内皮细胞血管舒缩因子表达影响的实验研究［J］．中国中药杂志，2006，31(7)：566-569.

相似文献/References:

[1]费燕,林秀丽,胡永狮,等.柱前衍生-HPLC法测定人血清中丙戊酸钠的方法改进[J].医学研究与战创伤救治(原医学研究生学报),2010,12(05):418.
　FEI Yan,LIN Xiu-li,HU Yong-shi,et al.Improvement of precolumn derivatization HPLC assay method in determination of sodium valproate in human serum[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2010,12(02):418.
[2]钟桂香,严 佳,黄爱文,等.高效液相色谱法测定氯化铵甘草口服溶液中吗啡、愈创甘油醚和甘草酸的含量[J].医学研究与战创伤救治(原医学研究生学报),2014,16(03):229.[doi:10.3969/j.issn.1672-271X.2014.03.002]
　ZHONG Gui-xiang,YAN Jia,HUANG Ai-wen,et al.Determination of morphine,guaifenesin and glycyrrhizic acid in ammonium glycyrrhiza oral solution by HPLC[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2014,16(02):229.[doi:10.3969/j.issn.1672-271X.2014.03.002]
[3]崔恩忠,陈美惠,贡 磊,等.寒痹丸的质量标准研究[J].医学研究与战创伤救治(原医学研究生学报),2016,18(03):281.[doi:10.3969/j.issn.1672-271X.2016.03.018]
　CUI En-zhong,CHEN Mei-hui,GONG Lei,et al.Study onquality standard of Hanbi Pill[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2016,18(02):281.[doi:10.3969/j.issn.1672-271X.2016.03.018]
[4]唐安福,苏倩雯,贡磊,等.HPLC法测定儿肾康丸中的特女贞苷含量[J].医学研究与战创伤救治(原医学研究生学报),2017,19(01):13.[doi:10.3969/j.issn.1672-271X.2017.01.004]
　TANG An-fu,SU Qian-wen,GONG Lei,et al.HPLC determination of specnuezhenide in Ershenkang pill[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2017,19(02):13.[doi:10.3969/j.issn.1672-271X.2017.01.004]
[5]杨八十,杨丽娜,叶丽娟,等.医院外用液体制剂配制分装系统的清洁验证[J].医学研究与战创伤救治(原医学研究生学报),2018,20(04):446.[doi:10.3969/j.issn.1672-271X.2018.04.029]
[6]汤淏,唐安福,贡磊,等.复方猕猴桃根颗粒质量标准提高的研究[J].医学研究与战创伤救治(原医学研究生学报),2019,21(02):163.[doi:10.3969/j.issn.1672-271X.2019.02.011]
　TANGHao,TANGAn-fu,GONGLei,et al.Study on improvement of quality standard of Fufang Mihoutao Granule[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2019,21(02):163.[doi:10.3969/j.issn.1672-271X.2019.02.011]
[7]骆雨璇,吴苏亚,陈醒,等.高效液相色谱法测定头孢克肟的含量[J].医学研究与战创伤救治(原医学研究生学报),2019,21(6):621.[doi:10.3969/j.issn.1672-271X.2019.06.015]
　LUO Yu-xuan,WU Su-ya,CHEN Xing,et al.Determination of cefixime by HPLC[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2019,21(02):621.[doi:10.3969/j.issn.1672-271X.2019.06.015]

备注/Memo

备注/Memo:
-
更新日期/Last Update: 2015-03-20