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[1]陈凯凯,郭和清,穆大为,等.模拟加速度对肾草酸钙结石模型大鼠氧化应激的影响[J].医学研究与战创伤救治(原医学研究生学报),2016,18(01):10-12,31.[doi:10.3969/j.issn.1672-271X.2016.01.003]
　CHEN Kai-kai,GUO He-qing,MU Da-wei,et al.Effect of acceleration on oxidative stress of renal calcium oxalate rat models[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2016,18(01):10-12,31.[doi:10.3969/j.issn.1672-271X.2016.01.003]
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模拟加速度对肾草酸钙结石模型大鼠氧化应激的影响()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第18卷
期数:
2016年01期
页码:
10-12,31
栏目:
出版日期:
2016-01-20

文章信息/Info

Title:
Effect of acceleration on oxidative stress of renal calcium oxalate rat models
作者:
陈凯凯; 郭和清; 穆大为; 李建业; 严景民; 尹杏林; 张立铭
100142 北京，北京空军总医院泌尿外科
Author(s):
CHEN Kai-kai; GUO He-qing; MU Da-wei; LI Jian-ye; YAN Jing-min; YIN Xing-lin; ZHANG Li-ming.
Department of Urology, Air Force PLA General Hospital,Beijing 100142,China
关键词:
肾结石; 草酸钙; 加速度; 氧化应激; 大鼠
Keywords:
kidney calculi;  calciumoxalate;  acceleration;  oxidative stress;  rats
分类号:
R852
DOI:
10.3969/j.issn.1672-271X.2016.01.003
文献标志码:
A
摘要:
目的 探讨加速度暴露对肾草酸钙结石模型大鼠氧化应激的影响。方法 采用乙二醇饮水和氯化铵灌胃法诱导建立大鼠草酸钙结石模型，将40只8周龄Wistar健康雄性大鼠随机分为4组（每组10只）：空白对照组（A组）、单纯诱石组（B组）、加速度并诱石组（C组）和单纯加速度组（D组）。其中A组采用自来水饮水+生理盐水2 mL/d灌胃，B组采用1%乙二醇+2%氯化铵溶液2 mL/d灌胃，C组在B组的基础上给予加速度（+6G）暴露，D组单纯给予+6G暴露。各组大鼠在相同条件下饲养28 d后收集血液及双肾标本，左肾组织做石蜡切片HE染色，光镜下观察草酸钙结晶情况；右肾组织制成组织匀浆，测定血液和右肾组织匀浆中丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、还原性谷胱甘肽（GSH）和谷胱甘肽过氧化物酶（GSH-Px）含量，以了解氧化应激情况。结果 单纯诱石组和加速度并诱石组肾组织草酸钙结晶评分较空白对照组和单纯加速度组均显著增高(P<0.01)，加速度并诱石组结晶评分亦较单纯诱石组增高(P<0.01)。单纯诱石组和加速度并诱石组与空白对照组比较，大鼠血、肾组织MDA浓度显著升高(P<0.01)，血、肾组织SOD、CAT、GSH-Px浓度显著降低(P<0.01)。结论 加速度暴露可能是泌尿系结石形成的危险因素之一，其机制可能与加速度暴露引起大鼠肾脏组织氧化应激损伤有关。
Abstract:
Objective To investigate the effect of accelerationon on oxidative stress of renal calcium oxalate (CaOx) rat models. Methods The models of renal CaOx were established with Ethylene Glycol(EG) in drinking water and Ammonium Chloride(AC) by gavage. A total of 40 adult healthy male Wistar rats were randomly and averagely divided into 4 groups(10 of each): blank control group(A), stone forming group(B), stone forming with +6G exposure group(C) and +6G exposure naive group(D). Group A was established with drinking water and physiological saline by gavage (2 mL per day). Group B was established with 1% ethylene glycol in drinking water and 2％ ammonium chloride by garage(2 mL per day). Group C, with the foundation of group B, was exposed in+6G. Group D was exposed in +6G as same as group C. After feeding for 28 days, blood and renal samples were collected. The presence of CaOx crystals was scored in left renals under hematoxylin and eosin staining, and the level of malonddialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and glutathione Peroxidase (GSH) in the blood and right renal homogenate were measured as indicators of oxidative stress. Results For the formation of CaOx crystals, the score of group B and group C were significantly higher than group A and group D(P<0.01), and the score of group C were significantly higher than group B(P<0.01). For the parameter of oxidative stress, compared with group A, the concentration of MDA in blood and kidney tissues of group B significantly increased (P<0.01),which significantly increased (P<0.01) after the intervention of +6G (group C). Compared with group A, the content of SOD, CAT and GSH-Px in blood and kidney tissues of group B significantly decreased (P<0.01), which significantly increased (P<0.01) after the intervention of +6G (group C). Conclusion Acceleration would be one of the risk factor contributed to kidney stone formation. The mechanism is connected with the oxidative damage of renal tissue．

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备注/Memo

备注/Memo:
全军后勤科研“十二五”重大项目(AKJ11J004)
更新日期/Last Update: 2016-01-20