|本期目录/Table of Contents|

[1]周 磊,范茂丹,史承勇.高同型半胱氨酸血症对大鼠颈总动脉平滑肌细胞增殖及Caspase-12蛋白表达的影响[J].医学研究与战创伤救治(原医学研究生学报),2016,18(01):21-24,46.[doi:10.3969/j.issn.1672-271X.2016.01.006]
　ZHOU Lei,FAN Mao-dan,SHI Cheng-yong..Effect ofhyperhomocystinemia on the proliferation of rats common carotid artery vascular smooth muscle cells and expression of Caspase-12[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2016,18(01):21-24,46.[doi:10.3969/j.issn.1672-271X.2016.01.006]
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高同型半胱氨酸血症对大鼠颈总动脉平滑肌细胞增殖及Caspase-12蛋白表达的影响()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第18卷
期数:
2016年01期
页码:
21-24,46
栏目:
出版日期:
2016-01-20

文章信息/Info

Title:
Effect ofhyperhomocystinemia on the proliferation of rats common carotid artery vascular smooth muscle cells and expression of Caspase-12
作者:
周 磊1; 范茂丹1; 史承勇2
1. 310002 浙江杭州，南京军区杭州疗养院海勤疗养区干部疗养科；
2. 310013 浙江杭州，解放军117医院心血管内科
Author(s):
ZHOU Lei1;  FAN Mao-dan1;  SHI Cheng-yong2.
1.Department of Convalescent, Naval Logistics Branch of Hangzhou Sanatorium, Nanjing Military Command， Hangzhou, Zhejiang 310002, China；
2. Department of Cardiology, 117 Hospital of PLA, Hangzhou, Zhejiang 310013, China
关键词:
同型半胱氨酸; 平滑肌细胞; 动脉粥样硬化; Caspase-12
Keywords:
homocysteine;  smooth muscle cell;  atherosclerosis;  Caspase-12
分类号:
R543
DOI:
10.3969/j.issn.1672-271X.2016.01.006
文献标志码:
A
摘要:
目的 研究高同型半胱氨酸血症对大鼠颈总动脉平滑肌细胞增殖及Caspase-12蛋白表达的影响。方法 选取雄性SD大鼠40只，随机数字表法分为4组，每组10只。分 别以不同剂量蛋氨酸饮食喂饲12周后分离颈总动脉。HE染色观察颈总动脉增殖情况；利用免疫荧光染色法观察并测定颈总动脉内增殖细胞核抗原（proliferating cell nuclear antigen, PCNA）表达情况；利用免疫组织化学染色法及蛋白免疫印迹法检测Caspase-12蛋白表达情况。结果 不同剂量蛋氨酸饮食导致大鼠颈总动脉不同程度增殖，颈总动脉内 PCNA、Caspase-12蛋白表达增加，并呈浓度依赖性。结论 高同型半胱氨酸血症通过激活血管平滑肌细胞内质网应激反应导致Caspase-12蛋白表达增加，加重血管损伤，促进平滑 肌细胞增殖，进一步加重动脉粥样硬化。
Abstract:
Objective To test the effect of hyperhomocystinemia on the proliferation of rats carotid artery vascular smooth muscle cells and the expression of Caspase-12. Methods Forty male SD rats were divided into four groups randomly. The common carotid artery were separated after feeding with high methionine foods for three months and taken to HE staining for observing the proliferation of carotid arteries. The expression of proliferating cell nuclear antigen (PCNA) was detected by immunofluorescence staining. The expression of Caspase-12 was tested by immunohistochemistry and Western blot. Results Different levels of methionine in the diet leaded to varying degrees of proliferation of common carotid artery smooth muscle cells and increased expression of PCNA and Caspase-12 in a dose-dependent manner. Conclusion Hyperhomocysteinemia can lead to increased expression of Caspase-12, which aggravates vascular injury through activation of endoplasmic reticulum stress response, promotes smooth muscle cell proliferation and further leads to atherosclerosis.

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2016-01-20