|本期目录/Table of Contents|

[1]郭利利,张葵.尿沉渣分析仪检测白细胞和细菌对尿路感染的筛查价值[J].医学研究与战创伤救治(原医学研究生学报),2017,19(03):234-238.[doi:10.3969/j.issn.1672-271X.2017.03.003]
　GUO Li-li,ZHANG Kui.The value of urine sediment analyzer in screening white blood cells and bacteria for urinary tractinfection[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2017,19(03):234-238.[doi:10.3969/j.issn.1672-271X.2017.03.003]
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尿沉渣分析仪检测白细胞和细菌对尿路感染的筛查价值()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第19卷
期数:
2017年03期
页码:
234-238
栏目:
出版日期:
2017-05-20

文章信息/Info

Title:
The value of urine sediment analyzer in screening white blood cells and bacteria for urinary tractinfection
作者:
郭利利; 张葵
作者单位：210008南京，南京鼓楼医院检验科
Author(s):
GUO Li-li; ZHANG Kui
（Department of Medical Laboratory，Drum Tower Hospital，Nanjing 210008，Jiangsu，China)
关键词:
尿路感染; 白细胞; 细菌; 中段尿培养; ROC曲线
Keywords:
Urinary tract infection;  White blood cells;  Bacteria;  Mid-stream urine culture;  ROC curve
分类号:
R446.12
DOI:
10.3969/j.issn.1672-271X.2017.03.003
文献标志码:
A
摘要:
目的 评估Uf-1000i尿沉渣分析仪检测白细胞和细菌的基本性能及对尿路感染的筛查价值。方法 收集304例疑是尿路感染做尿培养的患者的中段尿，用尿沉渣分析仪Uf-1000i检测尿白细胞（WBC）和细菌(BACT)。分析两项参数的精密度、线性和携带污染率。以尿细菌培养阳性为金标准，绘制尿白细胞和细菌计数的ROC曲线，并评价其诊断尿路感染的敏感度、特异度、假阳性率、假阴性率、阳性预期值、阴性预期值。结果 Uf-1000i检测白细胞和细菌的批内精密度、日间精密度、携带污染率和线性范围均符合厂家标准；Uf-1000i尿沉渣分析仪用于诊断尿路感染的阈值，为白细胞计数173.5个/μL和细菌计数513.5个/μL。若以白细胞或细菌任意一项阳性来判断尿路感染，敏感度、特异度、假阳性率、假阴性率、阳性预期值、阴性预期值分别为83.3%、73.0%、27.0%、16.7%、62.9%、88.8%。以白细胞和细菌双阳性来判断尿路感染，敏感度、特异度、假阳性率、假阴性率、阳性预期值、阴性预期值分别为54.6%、93.9%、6.1%、45.4%、83.1%、79.0%。结论 Uf-1000i尿沉渣分析仪检测白细胞和细菌的性能符合厂家标准，对尿路感染能早期筛查，便于临床医师决定是否需要培养及选择早期用药。
Abstract:
Objective To evaluate the performance of white blood cells (WBC) and bacteria (BACT) analyses in urinalysis analyzer Uf-1000i and determine the potential application for urinary tract infection screening.Methods A total of 304 cases with suspected urine infection were collected. The performance including precision, linearity and carryover contamination rates in Uf-1000i were determined. ROC curve was determined, and the sensitivity, specificity, false negative rates, expected positive rate and expected negative rate were evaluated compared with gold standard method-urine culture.Results Uf-1000i showed good performance of within-run precision, intra-day precision, carryover, and linearity range for both WBC and BACT. The cutoff value based on ROC analysis for urinary infection using Uf-1000i was further determined: 173.5 WBC/μL and 513.5 bacteria/μL. The sensitivity, specificity, false positive rate, false negative rate, positive predictive value, negative predictive for either WBC or BACT in Uf-1000i were 83.3%, 73.0%, 27.0%, 16.7%, 62.9%, 88.8%, respectively. The sensitivity, specificity, false positive rate, false negative rate, positive predictive value, negative predictive for both WBC and BACT in Uf-1000i were 54.6%，93.9%, 6.1%, 45.4%, 83.1%, 79.0%, respectively.Conclusion The performance of WBC and BACT analysis in Uf-1000i fulfilled the requirement. Therefore, it was able to be applied as rapid screening equipment for clinical urinary infection, which facilitates identification of bacteria species and offers guidance of early drug administration during clinical practice.

参考文献/References:

[1]马泽萍.膀胱冲洗对留置尿管尿路感染的影响[J]. 右江医学，2010，38(3)：307-308.
[2]张贤芝. 尿液不同检测方法对泌尿系统感染的诊断价值[J]. 中华实用诊断与治疗杂志，2011,25(3):265-266.
[3]Wang J, Zhang Y, Xu DW，et al. Evaluation of the Sysmex UF-1000i for the diagnosis of urinary tract infection[J]. Am J Clin Pathol，2010，133（4）:577-582.
[4]Okaha H, Sakai Y, Miy azaki S, et al. Detection of significant bacteriuria automated urinalysis using flow cytometry[J]. J Clin Microbiol, 2000, 38(8):2870-2872.
[5]王效雷，罗婕，丁兆霞，等.119例尿导管相关性尿路感染的病原体分布及耐药性分析[J]. 东南国防医药，2015,17(1):46-48.
[6]卢国光，方美丹，阮奕，等.ROC曲线在UF-1000i尿沉渣分析仪筛查尿路感染中的价值[J]. 浙江实用医学，2012,17(5):328-332.
[7]王建华.尿沉渣分析仪快速诊断尿路感染的可靠性研究[J]. 现代诊断与治疗，2016,27(16):3078-3079.
[8]杭建峰，孙朝晖，石玉玲，等. Sysmex Uf-1000i全自动尿沉渣分析仪的性能分析及临床应用[J]. 检验医学，2011,21(2):108-110.
[9]李丽娜，于敬达，程子英. UF-50全自动尿沉渣分析仪各检测项目干扰因素分析[J]. 包头医学院学报, 2010, 26(1):25.
[10]谢仿云，王莹超. 全自动尿沉渣分析仪在尿路感染诊断中的价值[J]. 检验医学与临床，2015,12(3):391-393.
[11]董青，张芸燕，缪杨阳，等. 尿流式细胞分析仪在尿路感染筛选中的应用和研究[J]. 国际检验医学杂志,2012,33(21):2646-2647.
[12]黄春光，杨洁飞，李小平. Uf-1000i尿沉渣分析系统在尿路感染快速诊断中的应用[J]. 实验与检验医学, 2009, 27(5):489.

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备注/Memo

备注/Memo:
基金项目：南京市医学科技发展重点项目（ZKX13028）
更新日期/Last Update: 2017-05-20