|本期目录/Table of Contents|

[1]谢树红,黄惠娟,许玉黎,等.橄榄苦苷对体外卵巢癌SKOV3细胞增殖的影响[J].医学研究与战创伤救治(原医学研究生学报),2018,20(04):361-364.[doi:10.3969/j.issn.1672-271X.2018.04.008]
　XIE Shu-hong,HUANG Hui-juan,XU Yu-li,et al.Effects ofoleuropein on proliferation of ovarian cancer SKOV3 cells in vitro[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2018,20(04):361-364.[doi:10.3969/j.issn.1672-271X.2018.04.008]
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橄榄苦苷对体外卵巢癌SKOV3细胞增殖的影响()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第20卷
期数:
2018年04期
页码:
361-364
栏目:
基础研究
出版日期:
2018-07-20

文章信息/Info

Title:
Effects ofoleuropein on proliferation of ovarian cancer SKOV3 cells in vitro
作者:
谢树红; 黄惠娟; 许玉黎; 郑小花; 杨帆
作者单位：350025福州，南京军区福州总医院妇产科（谢树红、黄惠娟、许玉黎、郑小花、杨帆）
Author(s):
XIE Shu-hong; HUANG Hui-juan; XU Yu-li; ZHENG Xiao-hua; YANG Fan
(Department of Obstetrics and Gynecology,Fuzhou General Hospital of Nanjing Military Region, PLA, Fuzhou 350025,Fujian,China)
关键词:
橄榄苦苷; 卵巢癌; 细胞增殖
Keywords:
oleuropein;  ovarian cancer; cell proliferation
分类号:
R915
DOI:
10.3969/j.issn.1672-271X.2018.04.008
文献标志码:
A
摘要:
目的 探讨不同浓度的橄榄苦苷在不同作用时间对卵巢癌SKOV3细胞增殖的影响。方法 SKOV3卵巢癌细胞传代培养后接种于3块96孔板，每板实验组细胞用100、200、400、800 μg/mL的橄榄苦苷培养液，对照组细胞用McCoy′s 5A完全培养基。CCK-8检测法测定24、48、72h等3个时间点不同药物浓度的SKOV3细胞增殖情况。结果 相同作用时间下，与对照组比较，100、200、400、800 μg/mL的橄榄苦苷浓度对SKOV3细胞的增殖均有抑制作用，细胞生长A值随着药物浓度增大而减小（P＜0
Abstract:
Objective To investigate the effects of different concentration of oleuropein on proliferation of ovarian cancer cell SKOV3 in different time.Methods SKOV3 ovarian cancer cells were inoculated in 3 blocks of 96 well plate. Cells of each trial group were treated with 100, 200, 400, 800 μg/mL oleuropein and vehicle, respectively. The proliferation of SKOV3 cells was determined by using CCK-8 assays after the treatment with indicated concentration of oleuropein for 24, 48, and 72 h.Results 100, 200, 400, and 800 μg/mL oleuropein significantly inhibited the proliferation of SKOV3 cells. Optical density (OD) was decreased accompanying with the dose increase of oleuropein (P<005). ODs of SKOV3 cells treated with 100, 200, and 400 μg/mL oleuropein were increased for the indicated incubation time (P<005). The proliferation of cells treated with 800 μg/mL oleuropein for 72 h was retarded compared with those for 24 h and 48 h (P<005). The inhibition rate of cell proliferation was increased with the dose increase of oleuropein treatment (P<005). However, there was no significant correlation with the incubation time (P>005).Conclusion Oleuropein could inhibit the proliferation of ovarian cancer cell SKOV3, the specific mechanisms needs further experimental study.

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备注/Memo

备注/Memo:
基金项目：全军医药卫生课题（14MS129）；福建省科技计划项目（2014Y5008）
更新日期/Last Update: 2018-07-20