|本期目录/Table of Contents|

[1]薛书生,笪虎,吴琪,等.挛缩肢体深筋膜中Ⅰ型胶原的表达[J].医学研究与战创伤救治(原医学研究生学报),2018,20(06):566-570.[doi:10.3969/j.issn.1672-271X.2018.06.002]
　XUE Shu-sheng,DA Hu,WU Qi,et al.Research on type-I collagen expression in deep fascia of contracture limbs[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2018,20(06):566-570.[doi:10.3969/j.issn.1672-271X.2018.06.002]
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挛缩肢体深筋膜中Ⅰ型胶原的表达()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第20卷
期数:
2018年06期
页码:
566-570
栏目:
基础研究
出版日期:
2018-11-20

文章信息/Info

Title:
Research on type-I collagen expression in deep fascia of contracture limbs
作者:
薛书生; 笪虎; 吴琪; 赵凤朝
作者单位：221004徐州,徐州医科大学（薛书生、赵凤朝）；223001淮安，解放军第八二医院骨科（笪虎、吴琪）
Author(s):
XUE Shu-sheng1; DA Hu 2; WU Qi2; ZHAO Feng-chao1
(1. Xuzhou Medical University, Xuzhou 221004,Jiangsu,China;2.Department of Orthopedics, the 82th Hospital of PLA, Huaian 223001, Jiangsu, China)
关键词:
制动性挛缩; 深筋膜; Ⅰ型胶原; 延展性; 牵引
Keywords:
immobilized contracture;  deep fascia;  type-I collagen;  malleability;  traction
分类号:
R34
DOI:
10.3969/j.issn.1672-271X.2018.06.002
文献标志码:
A
摘要:
目的 研究四肢发生制动性挛缩后，深筋膜组织内Ⅰ型胶原表达的变化以及肢体不易被拉伸的原因。 方法 将兔的右侧股骨干造成重叠短缩型骨折作为实验组，未作处理的左后肢作为对照组。1个月和2个月后分别切取双后肢股部外侧中段深筋膜，利用天狼猩红染色、免疫组化染色来观察含有Ⅰ型胶原的纤维束的显微形态，采用Western blot法检测Ⅰ型胶原的表达、实时荧光定量PCR法测定Ⅰ型胶原相关基因的表达的情况。 结果 实验组骨折后1个月和2个月的Ⅰ型胶原纤维长度均明显小于对照组[（58.3±18.5）μm vs （81.7±13.4）μm，（51.1±16.2）μm vs （83.5±14.6）μm，P<0.01]。与对照组比较，实验组骨折后1个月和2个月的Ⅰ型胶原表达量明显减少（P<0.05），Ⅰ型胶原蛋白mRNA的相对表达量明显下降（P<0.05）。结论 肢体挛缩后深筋膜内Ⅰ型胶原含量降低并导致肢体软组织延展性下降。
Abstract:
Objective Research the reason for change of type-I collagen expression change and difficulty of limbs to be stretched in the deep fascia after immobilizing contracture.Methods The right femoral shaft of rabbits was introduced to overlapping and shortened fracture as the experimental group. The left thigh without treatment was introduced as the control group. After 1 month and 2 months, the deep lateral fascia of the lateral thigh was cut. Morphology of fibrous bundles containing type I collagen was observed by Sirius red staining and immunohistochemical staining. The expression of type I collagen was detected by Western blot, and the expression of type I collagen-related gene was detected by real-time quantitative PCR.Results Whether it was one or two months after the fracture, the fiber bundles containing type I collagen of the deep fascia tissue were significantly shorter [（58.3±18.5）μm vs （81.7±13.4）μm，（51.1±16.2）μm vs （83.5±14.6）μm，P<0.01], the expression of type I collagen was significantly reduced (P<0.05) and the mRNA transcription level of type I collagen-related genes was significantly decreased (P<0.05) in the experimental group with limb contracture compared to that in the control group without intervention.Conclusion The content of type I collagen in deep fascia was decreased after limb contracture, leading to the decreased malleability of limb soft tissues.

参考文献/References:

[1]Derrick M，Knapik，BA，Joshua D，et al．The basic science of continuous passive motion in promoting knee health: a systematic reviewof studies in a rabbit model［J］．Arthroscopy，2013，29(10):1722-1731．
[2]胥少汀，葛宝丰，徐印坎．实用骨科学［M］.3 版.北京:人民军医出版社，2005: 2191-2194．
[3]漆佳丽, 冯海燕, 梁朋.Ⅰ型胶原蛋白α1链的真核表达及其与成纤维细胞的作用[J].生物技术, 2016，26(3):239-244,299.
[4]Tresoldi I，Oliva F，Benvenuto M，et al.Tendon’s ultrastructure［J］．Muscles Ligaments Tendons J，2013，3(1): 2-6.
[5]程津津,任萍萍.学习《关于善待实验动物的指导性意见》后的体会[J].实验动物科学,2011,28(3):78-79.
[6]孙伟, 艾红军, 张广道,等.钙磷涂层处理对镁合金植入体周围软组织I型胶原mRNA表达影响动物实验研究[J].中国实用口腔科杂志, 2012, 5(5):291-294.
[7]金长鑫,吴琼,刘烨,等.富血小板血浆凝胶联合脂肪干细胞促进大鼠创面修复的实验研究[J].东南国防医药,2016,18(4):349-353.
[8]Kobayashi K, Takahashi N, Jimi E,et al.Tumor necrosis factor alpha stimulates osteoclast differentiation by a mechanism independent of the ODF/RANKL-RANK interaction［J］.J Exp Med，2000，191(2):275-286.
[9]赵良军, 彭芬芬, 庄薇,等.骨质疏松大鼠膝关节组织总胶原、Ⅰ型及Ⅱ型胶原蛋白的含量变化[J].中国组织工程研究, 2011, 15(2):205-207.
[10]赵华昆, 马燕红.肌腱挛缩机制在分子水平的研究进展[J].中国康复, 2012, 27(2):127-129.
[11]卢天健, 徐峰.皮肤的力学性能概述[J].力学进展, 2008, 38(4):393-426.
[12]罗微, 代伟, 马兰,等.胡克定律探究实验装置的改进[J].物理实验, 2015(3):18-20.
[13]Mortazavian S, Fatemi A.Effects of fiber orientation and anisotropy on tensile strength and elastic modulus of short fiber reinforced polymer composites[J].Compos Part B Eng, 2015, 7(2):116-129.
[14]曹雪倩,张章,王林农.角膜生物力学测量及其临床应用新进展[J].医学研究生学报,2016,29(3):319-322.
[15]边蔷, 胡海威, 温建民,等.足部相关肌肉、肌腱组织材料弹性模量的测定[J].中国组织工程研究, 2015, 19(12):1919-1923.
[16]张剑锋, 陈允震, 刘海春,等.骨胶原在骨质疏松大鼠中的变化及其与骨生物力学相关性实验研究[J].中国矫形外科杂志, 2006, 14(23):1807-1809.
[17]郝建秀, 王海刚, 王伟宏,等.利用弹性体增韧木粉/HDPE复合材料[J].复合材料学报, 2016, 33(5):976-983.
[18]Ma L,Wang MZ,Ge XW. Surface treatment of poly(ethylene terephthalate) by gamma-ray induced graft copolymerization of methyl acrylate and its toughening effect on poly(ethylene terephthalate) /elastomer blend[J].Radiat Phys Chem, 2013, 9(10):92-97.
[19]马淑梅,陈瑶,刘莉.肌肉萎缩症治疗的研究进展[J].世界临床药物,2017,38(11):790-792，S73-S74.

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备注/Memo

备注/Memo:
基金项目：淮安市市级科技计划项目（HAB201731）
更新日期/Last Update: 2018-11-20