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[1]胡博森,张卓,王晓红,等.矢车菊素?3?葡萄糖苷促进成骨细胞MC3T3?E1增殖的作用机制[J].医学研究与战创伤救治(原医学研究生学报),2019,21(6):581-585.[doi:10.3969/j.issn.1672-271X.2019.06.007]
　HU Bo-sen,ZHANG Zhuo,WANG Xiao-hong,et al.Cyanidin-3-O-glucoside promotes proliferation of osteoblast MC3T3-E1 without Wnt/β-catenin pathway[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2019,21(6):581-585.[doi:10.3969/j.issn.1672-271X.2019.06.007]
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矢车菊素?3?葡萄糖苷促进成骨细胞MC3T3?E1增殖的作用机制()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第21卷
期数:
2019年6期
页码:
581-585
栏目:
基础研究
出版日期:
2019-11-14

文章信息/Info

Title:
Cyanidin-3-O-glucoside promotes proliferation of osteoblast MC3T3-E1 without Wnt/β-catenin pathway
文章编号:
1672-271X（2019）06-0581-05
作者:
胡博森;  张卓;  王晓红;  周波
110034 沈阳，沈阳医学院营养与食品卫生学教研室
Author(s):
HU Bo-sen;  ZHANG Zhuo;  WANG Xiao-hong;  ZHOU Bo
(Department of Nutrition and Food Hygiene,Shenyang Medical College,Shenyang 110034,Liaoning,China)
关键词:
矢车菊素?3?葡萄糖苷;  Wnt信号通路;  成骨细胞;  MC3T3?E1;  骨质疏松症
Keywords:
Cyanidin-3-O-glucoside;  Wnt signaling pathway;  osteoblasts;  MC3T3-E1;  osteoporosis
分类号:
R580
DOI:
10.3969/j.issn.1672-271X.2019.06.007
文献标志码:
A
摘要:
目的 探究矢车菊素-3-葡萄糖苷(C3G)促进成骨细胞MC3T3-E1细胞增殖的作用，以及对Wnt/β-catenin信号通路的影响。 方法 设置分组为对照组（C3G浓度为0 μmol/L）和不同浓度C3G组（25、50、100、200和400 μmol/L），在无血清培养基同步化处理后孵育24 h、48 h和72 h，使用MTT法测定细胞增殖率，实时细胞分析(RTCA)术收集96 h内细胞生长产生的电信号绘制时间-细胞指数散点图。另设置分组Wnt-C59-C3G组、DMSO-C3G组、Wnt-C59-对照组和DMSO-对照组，使用Wnt/β-catenin特异性抑制剂预处理4 h，比较C3G在5、10、25、50 μmol/L浓度下抑制剂下促成骨细胞增殖作用的变化。使用Western blot测定C3G浓度分别为0 μmol/L（对照组）和100 μmol/L（C3G组）的组间细胞中β-catenin蛋白水平。 结果 与对照组相比，各浓度C3G组细胞增殖率提高(P<0.01)，其中24 h时各浓度组间增殖率差异达到最大。Wnt-C59抑制剂未对C3G促MC3T3-E1细胞增殖作用产生明显改变，各组间差异具有明显的统计学意义（F=22.913，P<0.001）。Western blot分析显示C3G组与对照组β-catenin蛋白水平差异无统计学意义(P=0.38)。 结论 C3G可以促进MC3T3-E1细胞增殖，其增殖作用可能未通过Wnt/β-catenin途径。
Abstract:
Objective To investigate whether Cyanidin-3-O-glucoside (C3G) promotes the proliferation of MC3T3-E1 cells and whether C3G regulates osteoblasts via Wnt/β-catenin signaling pathway. Methods The cells were grouped into control group (0 μmol/L) and C3G groups （25, 50, 100, 200 and 400 μmol/L）, then were incubated for 24 h, 48 h, and 72 h at a C3G concentration set in a group after treatment for the synchronized serum-free medium. The cell proliferation rate was measured using the MTT assay. Real-time call analysis (RTCA) was used to collect electrical signals generated by cell growth within 96 h and to plot time-cell index scatter plots. The groupings were Wnt-C59-C3G, DMSO-C3G, Wnt-C59-control and DMSO-control, respectively, and then pretreatment with Wnt/β-catenin inhibitor Wnt-C59 for 4 h. Comparison of the effects of C3G on the proliferation of osteoblasts induced by inhibitors at concentrations of 5, 10, 25, and 50 μmol/L. The levels of β-catenin in the cells with C3G concentration of 0 μmol/L (control group) and 100 μmol/L (C3G group) were determined by Western blotting. Results Compared with the control group, the cell proliferation rate of each concentration of C3G group was increased (P<0.01). Wnt-C59 inhibitor did not significantly change the proliferation of C3G-promoting MC3T3-E1 cells, and the difference between the groups was statistically significant (F=22.913, P<0.001). Western blot analysis showed that there was no statistically significant difference in the level of β-catenin protein between C3G group and control group (P=0.38). Conclusion C3G can promote MC3T3-E1 cell proliferation in a Wnt/β-catenin pathway-independent manner.

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备注/Memo

备注/Memo:
收稿日期：2019-05-05
更新日期/Last Update: 2019-11-15