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[1]刘容,方潇,高宇文,等.澳洲茄碱通过mTOR信号通路抑制膀胱癌细胞增殖[J].医学研究与战创伤救治(原医学研究生学报),2020,22(6):572-577.[doi:10.3969/j.issn.1672-271X.2020.06.004]
　LIU Rong,FANG Xiao,GAO Yu-wen,et al.Solasonine inhibits the proliferation of bladder cancer cells through mTOR signaling pathway[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2020,22(6):572-577.[doi:10.3969/j.issn.1672-271X.2020.06.004]
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澳洲茄碱通过mTOR信号通路抑制膀胱癌细胞增殖()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第22卷
期数:
2020年6期
页码:
572-577
栏目:
基础研究
出版日期:
2020-11-20

文章信息/Info

Title:
Solasonine inhibits the proliferation of bladder cancer cells through mTOR signaling pathway
作者:
刘容; 方潇; 高宇文; 林飞飞; 林志杰; 陈榕彬; 翁铭芳; 吴卫真
作者单位：350025福州，解放军联勤保障部队第九〇〇医院泌尿外科（刘容、陈榕彬、翁铭芳、吴卫真）；350025福州，福建医科大学福总临床医学院泌尿外科（方潇、高宇文、林飞飞）；350200福州，福州市长乐区医院泌尿外科（林志杰）
Author(s):
LIU Rong1;  FANG Xiao2;  GAO Yu-wen2;  LIN Fei-fei2;  LIN Zhi-jie3;  CHEN Rong-bin1;  WENG Ming-fang1;  WU Wei-zhen1
（1.Department of Urology, the 900th Hospital of Joint Logistics Support Force, PLA, Fuzhou 350025, Fujian, China；2.Department of Urology, Fuzong Clinical Medical College of Fujian Medical Universigy, Fuzhou 350025, Fujian, China；3. Department of Urology, Fuzhou Changle District Hospital, Fuzhou 350200, Fujian, China）
关键词:
澳洲茄碱; 膀胱癌; J82细胞; T24细胞; 细胞凋亡; 哺乳动物雷帕霉素靶蛋白
Keywords:
solasonine;  bladder cancer;  J82 cells;  T24 cells;  apoptosis;  mammalian target of Rapamycin
分类号:
R737.14
DOI:
10.3969/j.issn.1672-271X.2020.06.004
文献标志码:
A
摘要:
目的研究澳洲茄碱对膀胱癌细胞增殖、细胞凋亡、细胞周期的影响及其作用机制。方法0、5、10、20、40 μmol/L澳洲茄碱处理膀胱癌J82和T24细胞24 h、48 h、72 h后，应用CCK-8检测细胞生存率。0、10、30 μmol/L澳洲茄碱处理J82细胞24 h后，用流式细胞术检测细胞凋亡和细胞周期。0、10、30 μmol/L 澳洲茄碱处理J82细胞24 h后，应用Western blot法检测mTOR、p-mTOR、p70S6K、p-p70S6K蛋白的表达。0、10、20、30 μmol/L澳洲茄碱处理J82细胞24 h后，RT-qPCR法检测mTOR、p70S6K mRNA表达情况。结果澳洲茄碱显著抑制膀胱癌J82细胞、T24细胞增殖，相同作用时间、不同浓度组细胞生存率总体差异均有统计学意义（P<0.01）。澳洲茄碱诱导J82细胞凋亡，0、10、30 μmol/L澳洲茄碱组凋亡率分别为（0.93±0.26）%、（8.33±2.16）%、（29.53±7.63）%。澳洲茄碱引起J82细胞发生S期阻滞，0、10、30 μmol/L澳洲茄碱组S期细胞占比分别是（34.51±3.61）%、（39.34±3.21）%、（44.50±4.29）%。澳洲茄碱下调J82细胞p-mTOR蛋白表达（F=85.18，P<0.01）和p-p70S6K蛋白表达（F=74.96，P<0.01）。澳洲茄碱下调J82细胞mTOR mRNA表达（F=31.49，P<0.01）和p70S6K mRNA表达（F=47.08，P<0.01）。结论澳洲茄碱通过mTOR信号通路抑制膀胱癌细胞增殖、促进细胞凋亡、阻滞细胞周期。
Abstract:
ObjectiveTo study the effect of solasonine on the proliferation, apoptosis and cell cycle of bladder cancer cells and its mechanism.MethodsAfter the treatment of bladder cancer cells J82 and T24 with 0, 5, 10, 20 and 40 μmol/L solasonine for 24 h, 48 h and 72 h, CCK-8 was used to detect cell proliferation. After the treatment of J82 cells with 0, 10 and 30 μmol/L solasonine for 24 h, FACS was performed to detect cell cycle and apoptosis. After the treatment of J82 cells with 0, 10, 20 and 30 μmol/L solasonine for 24 h, Western blot was undergone to detect the expression of mTOR, p-mTOR, p70S6K and p-p70S6K protein. After the treatment of J82 cells with 0, 10 and 30 μmol/L solasonine for 24 h, RT-qPCR was used to detect the expression of mTOR and p70S6K mRNA.ResultsSolasonine significantly inhibited the proliferation of bladder cancer cell J82 and T24 cells (P<0.01).Solasonine induced apoptosis of J82 cells, the apoptosis rates of 0, 10 and 30 μmol/L solasonine groups were (0.93±0.26）%, (8.33±2.16)% and (29.53±7.63)%, respectively. Solasonine arrested J82 cells at S stage, the proportions of S stage of 0, 10 and 30 μmol/L solasonine groups were (34.51±3.61)%, (39.34±3.21)% and (44.50±4.29)%, respectively. Solasonine downregulated the protein expression of p-mTOR of J82 cells (F=85.18, P<0.01). Solasonine downregulated the protein expression of p-p70S6K of J82 cells (F=74.96, P<0.01). Solasonine downregulated the mRNA expression of mTOR of J82 cells (F=31.49, P<0.01). Solasonine downregulated the mRNA expression of p70S6K of J82 cells (F=47.08, P<0.01).ConclusionSolasonine can inhibit the proliferation of bladder cancer cells, induce cell apoptosis and arrest cell cycle through mTOR signaling pathway.

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备注/Memo

备注/Memo:
基金项目：联勤保障部队第九〇〇医院2019年度医院科研计划项目（2019Q01）
更新日期/Last Update: 2020-12-01