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[1]倪晓霞,刘晓玲,杨育儒,等.复方黄连灌肠液总生物碱含量测定研究[J].医学研究与战创伤救治(原医学研究生学报),2021,23(01):41-44.[doi:10.3969/j.issn.1672-271X.2021.01.001]
　NI Xiao-xia,LIU Xiao-ling,YANG Yu-ru,et al.Optimization of determination for total alkaloids in compound Huanglian clysis[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2021,23(01):41-44.[doi:10.3969/j.issn.1672-271X.2021.01.001]
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复方黄连灌肠液总生物碱含量测定研究()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第23卷
期数:
2021年01期
页码:
41-44
栏目:
出版日期:
2021-01-20

文章信息/Info

Title:
Optimization of determination for total alkaloids in compound Huanglian clysis
作者:
倪晓霞; 刘晓玲; 杨育儒; 王庆芬
作者单位：363000漳州，解放军联勤保障部队第九○九医院/厦门大学附属东南医院制剂科（倪晓霞、刘晓玲、杨育儒、王庆芬）
Author(s):
NI Xiao-xia;  LIU Xiao-ling;  YANG Yu-ru;  WANG Qing-fen
(The Pharmacy Department, the 909th Hospital of PLA/the Affiliated Southeast Hospital of Xiamen University, Zhangzhou 363000, Fujian, China)
关键词:
复方黄连灌肠液; 柱色谱-紫外可见分光光度法; 总生物碱; 含量测定
Keywords:
compound Huanglian clysis; column chromatogram-UV spectrophotomatry; total alkaloids; content determination
分类号:
R917.74
DOI:
10.3969/j.issn.1672-271X.2021.01.001
文献标志码:
A
摘要:
目的研究并建立复方黄连灌肠液中总生物碱含量测定方法。方法采用柱色谱-紫外可见分光光度法，以盐酸小檗碱为对照品，采用盐酸-甲醇(1∶100)水浴加热回流提取法提取，提取液用中性氧化铝进行液-固萃取净化处理，于345 nm处测定其含量。结果盐酸小糪碱在1.616～19.392 μg/mL质量浓度范围内线性关系良好(r=0.999 98)，平均加样回收率100.86%、RSD为1.852%(n=6)。结论柱色谱-紫外分光光度法简便、快速、稳定可靠，适用于复方黄连灌肠液总生物碱的含量测定。
Abstract:
ObjectiveTo establish a method for the determination of the content of total alkaloids in compound Huanglian clysis.MethodsThe sample was extracted with HCl-CH3OH(1∶100) in water bath heating reflux extraction and total alkaloids were separated on an alumina column. The content of total alkaloids was determined by column chromatography-uv spectrophotometry at the detection wavelength of 345nm with berberine was used as the standard.ResultsA good linear relationship was observed in the concentration range of 1.616-19.392 μg/mL for berberine (r=0.999 98), and the average recovery rate was 100.86% with RSD of 1.852%(n=6).ConclusionThe method was simple, fast, stable and reliable，and it was proved to be applicable for the determination of the content of total alkaloids in compound Huanglian clysis.

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备注/Memo

备注/Memo:
基金项目：解放军第175医院青年苗圃课题项目（16Y025）
更新日期/Last Update: 2021-01-26