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[1]赵志刚,曹燕丽,高茗,等.姜黄素诱导转铁蛋白纳米载体体系的组装及其抗肿瘤靶向作用[J].医学研究与战创伤救治(原医学研究生学报),2023,25(2):160-163.[doi:10.3969/j.issn.1672-271X.2023.02.010]
　ZHAO Zhigang,CAO Yanli,GAO Ming,et al.Assembly of curcumin-induced transferrin nanocarrier system and its anti-tumor targeting[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2023,25(2):160-163.[doi:10.3969/j.issn.1672-271X.2023.02.010]
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姜黄素诱导转铁蛋白纳米载体体系的组装及其抗肿瘤靶向作用()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第25卷
期数:
2023年2期
页码:
160-163
栏目:
药学研究
出版日期:
2023-05-31

文章信息/Info

Title:
Assembly of curcumin-induced transferrin nanocarrier system and its anti-tumor targeting
作者:
赵志刚; 曹燕丽; 高茗; 龚光明; 恽时锋
作者单位：210002南京，东部战区总医院医疗保障中心实验动物室(赵志刚、恽时锋)，医疗保障中心药剂科(曹燕丽、高茗、龚光明)
Author(s):
ZHAO Zhigang1; CAO Yanli2; GAO Ming2; GONG Guangming2; YUN Shifeng1
(1.Laboratory of Comparative Medicine，2.Department of Pharmacy，Medical Support Center，General Hospital of Eastern Theater Command,PLA,Nanjing 210002,Jiangsu,China)
关键词:
转铁蛋白; 姜黄素; 药物递送; 自组装; 肿瘤靶向
Keywords:
transferrin; curcumin; drug delivery; assembly; tumor target
分类号:
R943
DOI:
10.3969/j.issn.1672-271X.2023.02.010
文献标志码:
A
摘要:
目的增加姜黄素(CCM)溶解性及对肿瘤的靶向性。方法以盐酸为变性剂，构建转铁蛋白(Tf)-CCM纳米粒子（Nanoparticles，NPs-CCM),电镜观察其外貌。对体系载药量、稳定性及其靶向性进行考察。结果 盐酸变性法成功构建了转铁蛋白-姜黄素纳米体系。Tf某些疏水基团充分暴露，引发了粒子的聚集。电镜观察粒子为150 nm左右的球形粒子，高效液相测定其载药量为5%，该纳米体系可使姜黄素的溶解度增加超过1万倍。CCM比NPs-CCM更容易降解，稳定性弱。NPs-CCM (0.05~1 mg/mL)的溶血效果明显低于游离CCM (P<0.05)，当NPs-CCM 与CCM的浓度在0.0025~0.001 mg/mL之间，两者的溶血性无显著差异。与CCM相比，NPs-CCM靶向性显著提高。结论NPs-CCM体系对肿瘤细胞具有良好的靶向性，具有潜在的临床运用价值。
Abstract:
ObjectiveTo increase the solubility and targeting capability of curcumin (CCM) in tumor.MethodsHydrochloric acid was used as denaturing agent，transferrin (Tf)-CCM was constructed and its appearance was observed under electron microscope. The drug loading，stability and targeting of the system were investigated.ResultsThe nanoscale system of Tf-CCM was successfully constructed by hydrochloric acid denaturation method. The spherical particles of about 150 nm were observed by electron microscope，and the drug loading was 5% detected by HPLC. The changes in the fluorescence spectra of Tf and 1-(anilinon)-aphthalene-8-sulfonic acid (ANS) in the NP-CCM preparation indicated that the polarity of certain hydrophobic and hydrophilic groups of Tf changed. The nanosystem increased the solubility of curcumin by more than 10,000 times and improved its targeting ability.ConclusionTransferrin-curcumin system has good targeting activity to tumor cells and has potential clinical application value.

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相似文献/References:

[1]朱庆,曹燕丽,徐云燕,等.酸变性法构建转铁蛋白?姜黄素纳米粒子及其肿瘤靶向性[J].医学研究与战创伤救治(原医学研究生学报),2019,21(3):262.[doi:10.3969/j.issn.1672-271X.2019.03.009]
　ZHU Qing,CAO Yan-li,XU Yun-yan,et al.Construction of transferrin curcumin nanoparticles by acid denaturation and its tumor targeting[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2019,21(2):262.[doi:10.3969/j.issn.1672-271X.2019.03.009]

备注/Memo

备注/Memo:
基金项目：国家自然科学基金（31671026）
更新日期/Last Update: 2023-07-24