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[1]蔡少甫,郑庆亦,胡安根,等.高密度脂蛋白对严重烫伤大鼠肾功能的 保护作用[J].医学研究与战创伤救治(原医学研究生学报),2008,10(03):164.
　CAI Shao-fu,ZHENG Qing-yi,HU An-gen,et al.The protective effect of high density lipoprotein on the renal injury of rats with severe burn[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2008,10(03):164.
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高密度脂蛋白对严重烫伤大鼠肾功能的 保护作用()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第10卷
期数:
2008年03期
页码:
164
栏目:
出版日期:
2008-05-20

文章信息/Info

Title:
The protective effect of high density lipoprotein on the renal injury of rats with severe burn
文章编号:
1672-271X(2008)03-0164-04
作者:
蔡少甫; 郑庆亦; 胡安根; 张闽峰; 陈锦河; 郑健生
解放军第175医院南京军区烧伤整形研究所，福建漳州 363000
Author(s):
CAI Shao-fu;  ZHENG Qing-yi;  HU An-gen;  ZHANG Min-feng;  CHEN Jin-he;  ZHENG Jian-sheng
The 175th Hospital of PLA, Zhangzhou 363000, Fujian, China
关键词:
烫伤; 肾脏; 脂蛋白类; HDL; 细胞间粘附分子-1; 肿瘤坏死因子-α
Keywords:
Burns;  kidney;  Lipoproteins;  HDL;  Intercellular adhesion molecules-1;  Tumor necrosis factor alpha
分类号:
R644
DOI:
-
文献标志码:
A
摘要:
目的 探讨高密度脂蛋白(HDL)对严重烫伤大鼠肾功能的保护作用。方法 135只Wistar大鼠随机分为对照组(n=15)、烫伤组(n=60)和实验组(n=60)。烫伤组和实验组大鼠背部造成30%TBSAⅢ度烫伤创面，实验组伤后即经尾静脉注入HDL(80 mg/kg)，烫伤组及实验组于伤后30 min经腹腔注射平衡液复苏，对照组不作处理。检测各组大鼠伤后12、24、48、72 h血清尿素氮(BUN)、肌酐(Cr)、氧化低密度脂蛋白(oxLDL)、细胞间粘附分子-1 (ICAM-1)及肿瘤坏死因子-α(TNF-α)含量；观察大鼠伤后48 h肾脏组织病理学变化。结果 烫伤组及实验组伤后各时相血清BUN、Cr、oxLDL、ICAM-1及TNF-α含量均明显高于对照组(Ｐ＜0.01)，但实验组含量明显低于烫伤组(Ｐ<0.05或0.01)。烫伤组伤后48 h光镜下肾小管上皮细胞肿胀明显、部分坏死，管腔内蛋白浓缩成管型，肾间质内炎性细胞浸润；电镜下肾小管细胞呈现典型凋亡现象。与烫伤组比较，实验组病理改变明显较轻。结论 HDL对严重烫伤大鼠肾功能损伤具有保护作用，可能与其抑制oxLDL生成和ICAM-1表达有关。
Abstract:
Objective To investigate the protective effect of high density lipoprotein on the renal injury of rats with severe burns. Methods One hundred and thirty-five Wistar rats were employed in the study and were randomly divided into control (n=15,without treatment), burn(n=60,with 30%TBSA full-thickness burn on the back) and experimental (n=60,with the injection of HDL(80 mg/kg) via the caudal vein immediately after burns) groups.The rats in the groups with burns injury were resuscitated with intraperitoneal isotonic saline(50 ml/kg)30 minutes after burn.The serum contents of BUN, Cr, oxLDL, ICAM-1 and TNF-α of the rats of all the three groups were determined with corresponding methods. The histological changes in the renal tissue of the rats in all groups were observed under light microscope and electronic microscope.Results The serum contents of BUN, Cr, oxLDL,ICAM-1 and TNF-α in the control group were obviously lower than those in burn group(P<0.01),while those in experimental group were also markedly lower than those in burn group(P<0.05 or 0.01). The renal tubule epithelial cell appeared swelling and necrosis, protein casts were found in the renal tubule, inflammatory cell infiltration were found in the glomerulus. Compared with the burn group, the renal injury was less marked in the experimental group at 48 hours postburn. Conclusion High density lipoprotein can be benefical to the protection of renal tissue in protecting from secondary injury rats with severe burns.

参考文献/References:

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备注/Memo

备注/Memo:
全军“十一五”计划课题资助项目(06MA107)
更新日期/Last Update: 2013-11-22