|本期目录/Table of Contents|

[1]陆春雷,吴国忠,尹天泉,等.细胞粘附在结肠癌细胞系SW480多药耐药表型中的作用[J].医学研究与战创伤救治(原医学研究生学报),2009,11(06):485-487，490.
　LU Chun-lei,WU Guo-zhong,YIN Tian-quan,et al.Roles of cell adhesion in multidrug resistance phenotype of colon cancer cell line SW480[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2009,11(06):485-487，490.
点击复制

细胞粘附在结肠癌细胞系SW480多药耐药表型中的作用()
分享到62.8K

《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第11卷
期数:
2009年06期
页码:
485-487，490
栏目:
出版日期:
2009-11-20

文章信息/Info

Title:
Roles of cell adhesion in multidrug resistance phenotype of colon cancer cell line SW480
文章编号:
1672-271X(2009)06-0485-04
作者:
陆春雷1 ; 吴国忠2 ; 尹天泉1 ; 同李平1 ; 丰 帆1 ; 郑琇山1 ; 张洪伟1
1.第四军医大学西京消化病医院消化一科，陕西西安 710032；2.解放军第101医院普通外科，江苏无锡 214044
Author(s):
LU Chun-lei1; WU Guo-zhong2; YIN Tian-quan1; TONG Li-ping1; FENG Fan1; ZHENG Xiu-shan1; ZHANG Hong-wei1
1. Xijing Hospital of Digestive Diseases, the Forth Military Medical University,Xi’an 710032, Shanxi,China;2.Department of Surgery,the 101th Hospital of PLA,Wuxi 214044,Jiangsu,China
关键词:
结肠癌; 层粘连蛋白; 细胞粘附介导耐药; 凋亡
Keywords:
Colon cancer;  Laminin;  Cell adhesion-mediated drug resistance;  Apoptosis
分类号:
R735.35
DOI:
-
文献标志码:
A
摘要:
目的 探讨细胞粘附在结肠癌细胞系SW480多药耐药表型中的作用。 方法 通过细胞粘附实验、四甲基偶氮唑盐实验(MTT 法)、细胞内阿霉素的蓄积和潴留及Annexin V/PI染色法检测凋亡等检测粘附于层粘连蛋白(LN)的结肠癌细胞系SW480的多药耐药性的改变。结果 粘附于LN的SW480细胞对化疗药物敏感性显著下降，化疗药物的IC50均显著升高，化疗药物诱导的凋亡指数明显减少；细胞内阿霉素的蓄积和潴留均明显减少，药物泵出率显著增加(P<0.05)。 结论 结肠癌细胞SW480与LN粘附后，可能提高了对药物转运的能力，导致化疗药物在肿瘤细胞内蓄积的减少；通过提高抗凋亡的能力，逃避化疗药物诱导的凋亡。
Abstract:
Objective To explore the roles of cell adhesion in multidrug resistance (MDR) of colon cancer cell line SW480.Methods The cell adhesion assay, MTT assay, Adriamycin (ADR) accumulation and retention and Annexin V/PI staining were used to illuminate the drug resistance of colon cancer cell line SW480 after adhesion to laminin (LN). Results After adhesion to LN, SW480 cells exhibited a significantly higher IC50, medicine pumping rate and a reduction of drug sensitivity, apoptosis index induced by chemotherapeutics and accumulation and retention of ADR (P<0.05). Conclusion Adhesion to LN may promote MDR phenotype of colon cancer cells by two means: The adhesion of SW480 to LN may promote the drug transport ability and decreases the accumulation of chemotherapeutic drugs in tumor cells;The adhesion may elevate the capability of anti-apoptosis and inhibit the apoptosis induced by chemotherapeutic drugs.

参考文献/References:

[1]Nelson J, McFerran NV, Pivato G,et al.The 67 kDa laminin receptor: structure, function and role in disease.[J] Biosci Rep，2008，28(1):33-48.
[2]Fan D, Zhang X, Chen X, et al. Bird's-eye view on gastric cancer research of the past 25 years[J]. J Gastroenterol Hepatol，2005，20(3):360-365.
[3]Kenneth H, Shain SD. Cell adhesion is a key determinant in de novo multidrug resistance (MDR): new targets for the prevention of acquired MDR[J]. Mol Cancer Ther, 2001，11(1):69-78.
[4]Damiano JS. Integrins as novel drug targets for overcoming innate drug resistance[J]. Curr Cancer Drug Targets，2002，2(1):37-43.
[5]Sethi T, Rintoul RC, Moore SM, et al. Extracellular matrix proteins protect small cell lung cancer against apoptosis: a mechanism for small cell lung cancer growth and drug resistance in vivo[J]. Nature Med,1999，5(6):662-668.
[6]Damiano JS, Halzehurst LA, Dalton WS. Cell adhesion-mediated drug resistance (CAM-DR) protects the K562 chronic myelogenous leukemia cell line from apoptosis induced by BCR/ABL inhibition,cytotoxic,drugs,and gamma-irridiation[J]. Leukemia, 2001, 15(8): 1232-1239.
[7]Stupack D, Cheresh DA. Get a ligand, get a life: integrins, signaling and cell survival[J]. J Cell Sci,2002，115(19):3729-3738.
[8]Martin SS, Vuori K. Regulation of Bcl-2 proteins during anoikis and amorphosis[J]. Biochimica et Biophysica Acta,2004，1692(2-3):145-147.
[9]Givant-Horwitz V,Davidson B,Reich R.Laminin-induced signal ing in tumor cells[J].Cancer Letters, 2005，223(1):1-10.
[10]Schmi dmaier R,Baumann P.ANTI-ADHESION evolves to a promising therapeutic concept in oncology[J].Curr Med Chem,2008,15(10):978-990.

相似文献/References:

[1]吴 曼,骆文香,崔向华,等.结肠癌宫颈转移1例[J].医学研究与战创伤救治(原医学研究生学报),2012,14(01):26.
[2]张胜行,张诗兰,兰小鹏.人结肠癌SW480细胞裸鼠皮下移植瘤模型的建立[J].医学研究与战创伤救治(原医学研究生学报),2012,14(02):108.
　ZHANG Sheng-hang,ZHANG Shi-lan,LAN Xiao-peng..The establishment of a subcutaneously transplanted tumor model with human colon cancer cell line SW480 in nude mice[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2012,14(06):108.
[3]梁 冲,王跃华,李增才,等.氟尿嘧啶缓释剂在老年结肠癌术后疗效的观察[J].医学研究与战创伤救治(原医学研究生学报),2010,12(04):299.
　LIANG Chong,WANG Yue-hua,LI Zeng-cai,et al.Research on implanting of 5-Fluorouracil sustained-release to prevent local recurrence of colon carcinomas in the old[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2010,12(06):299.
[4]张胜行,兰小鹏,易浔飞.靶向DcR3的siRNA抑制人结肠癌 裸鼠皮下移植瘤的实验研究[J].医学研究与战创伤救治(原医学研究生学报),2009,11(01):1.
　ZHANG Sheng-hang,LAN Xiao-peng,Yi Xun-Fei.Colon cancer subcutaneously inoculated in nude mice inhibited by siRNA to DcR3[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2009,11(06):1.
[5]冒晓蓓,刘小北,褚晓源,等.叉头框蛋白M1、环氧合酶2在结肠癌组织中的表达及相关性分析[J].医学研究与战创伤救治(原医学研究生学报),2014,16(03):225.[doi:10.3969/j.issn.1672-271X.2014.03.001]
　MAO Xiao-bei,LIU Xiao-bei,CHU Xiao-yuan,et al.Expression and correlation of FoxM1 and COX-2 in colorectal cancer[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2014,16(06):225.[doi:10.3969/j.issn.1672-271X.2014.03.001]

备注/Memo

备注/Memo:
-
更新日期/Last Update: 2013-11-20