|本期目录/Table of Contents|

[1]仇学明,杜云翔,罗红来,等.ZnPPⅨ对胃癌腹膜转移细胞凋亡的影响[J].医学研究与战创伤救治(原医学研究生学报),2009,11(06):495-498.
　QIU Xue-ming,DU Yun-Xiang,LUO Hong-lai,et al.Effect of ZnPPⅨ on apoptosis of human gastric cancer cell line with peritoneal metastasis[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2009,11(06):495-498.
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ZnPPⅨ对胃癌腹膜转移细胞凋亡的影响()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第11卷
期数:
2009年06期
页码:
495-498
栏目:
出版日期:
2009-11-20

文章信息/Info

Title:
Effect of ZnPPⅨ on apoptosis of human gastric cancer cell line with peritoneal metastasis
文章编号:
1672-271X(2009)06-0495-04
作者:
仇学明1; 杜云翔1; 罗红来1; 厉 琴1; 白飞虎2; 惠亮亮2
1. 解放军第82医院消化内科，江苏淮安 223001；2. 第四军医大学全军消化病研究所，陕西西安 710032
Author(s):
QIU Xue-ming1; DU Yun-Xiang1; LUO Hong-lai1; LI Qin1; BAI Fei-hu2; HUI Liang-liang2
1.Department of Gastrointestinal，the 82th Hospital of PLA,Huai'an 223001,Jiangsu,China;2.Institue of Digestive,Xijing Hospital of the Fourth Military Medical Uiniversity,Xi'an 710032,Shanxi,China
关键词:
胃癌; 腹膜转移; ZnPPⅨ; 凋亡
Keywords:
Gastric cancer;  Peritoneal metastasis;  ZnPPⅨ;  Apoptosis
分类号:
R735.2
DOI:
-
文献标志码:
A
摘要:
目的 研究ZnPPⅨ对人胃癌腹膜细胞GC9811-P凋亡的影响。方法 采用MTT法测定GC9811-P细胞在ZnPPⅨ作用48 h后生长抑制情况；流式细胞仪观察ZnPPⅨ对GC9811-P细胞周期和细胞凋亡的影响；western blot免疫印迹法观察ZnPPⅨ对细胞系CIAPIN1、Bcl-2、Bax蛋白表达水平的影响。结果 各浓度值的ZnPPⅨ 均可抑制GC9811-P细胞增殖,并促进肿瘤细胞凋亡，并降低 CIAPIN1和Bcl-2/Bax蛋白的表达水平。结论 ZnPPⅨ可诱导GC9811-P细胞的凋亡,其作用机制可能通过调控CIAPIN1基因影响Bcl-2/Bax分子的表达。
Abstract:
Objective To explore the effect of ZnPPⅨ on the apoptosis of human gastric cancer cell line GC9811-P with peritoneal metastasis. Methods After GC9811-P cells were treated with ZnPPⅨ for 48 hours, cell proliferation was detected using MTT assay. Flow cytometry was used to detect the apoptosis of GC9811-P cells, and western blot was used to check the expression of CIAPIN1, Bcl-2/Bax. Results ZnPPⅨ inhibited the proliferation of GC9811-P cells at the concentrations of 1×10-2 to 1×10-9 mol/L, and cell arrested in G1/S phase. The proportions of apoptosis cells were significantly increased compared with controls. The expression of CIAPIN1 and Bcl-2/Bax was decreased after incubated for 3h with 1×10-5 mol/L ZnPPⅨ. Conclusion ZnPPⅨ induce the apoptosis of gastric cancer cell lines GC9811-P with peritoneal metastasis by suppressing of the expression of CIAPIN1, and affection of its downstream apoptosis factors Bcl-2 and Bax.

参考文献/References:

[1]Bai F, Wu K, Fan D，et al.Inhibitory effects of a specific phage-displayed peptide on high peritoneal metastasis of gastric cancer[J].J Mol Med,2007,85(2):169-180.
[2]Feihu Bai, Kaichun Wu, Daiming Fan,et al.Establishment and characterization of a high metastatic potential in the peritoneum for human gastric cancer by orthotopic tumor cell Implantation[J].Dig Dis Sci,2007,52(6):1571-1578.
[3]Busserolles J,Megias J,Terencio MC,et al.Heme oxygenase-1 inhibits apoptosis in Caco-2cells via activation of Akt pathway[J].Int J Biochem Cell Biol,2006,38(9):1510-1517.
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[5]Liu ZM, Chen GG, Ng EK, et al. Upregulation of heme oxygenase-1 and p21 confers resistance to apoptosis in human gastric cancer cells [J].Oncogene,2004,23(2):503-513.
[6]Hao Z,Zhang G, Fan D，et al. Subcellular localization of CIAPIN1[J].J Histochem Cytochem,2006,54(12):1437-1444.
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备注/Memo

备注/Memo:
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更新日期/Last Update: 2013-11-20