|本期目录/Table of Contents|

[1]章仁品,宋晓蓉,翟志敏,等.超声观察骨髓间充质干细胞移植对兔心肌梗死心功能的影响[J].医学研究与战创伤救治(原医学研究生学报),2010,12(03):193-196.
　ZHANG Ren-pin,SONG Xiao-rong,ZHAI Zhi-min,et al.Observation of effect of transplantation of bone marrow mesenchymal stem cells on rabbit cardiac function after myocardial infarction by echocardiography[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2010,12(03):193-196.
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超声观察骨髓间充质干细胞移植对兔心肌梗死心功能的影响()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第12卷
期数:
2010年03期
页码:
193-196
栏目:
出版日期:
2010-05-20

文章信息/Info

Title:
Observation of effect of transplantation of bone marrow mesenchymal stem cells on rabbit cardiac function after myocardial infarction by echocardiography
文章编号:
1672-271X(2010)03-0193-04
作者:
章仁品1; 宋晓蓉2; 翟志敏3; 严中亚2
1.230031 安徽合肥，解放军105医院超声科；2.230001 安徽合肥，安徽省立医院胸外科；3.230022 安徽合肥，安徽医科大学附属第二医院血液科
Author(s):
ZHANG Ren-pin1; SONG Xiao-rong2; ZHAI Zhi-min3; YAN Zhong-ya2
1.Department of Ultrasound， 105 Hospital of PLA, Hefei，Anhui 230031,China;2.Department of Cardiovascular Surgery,Anhui Provincial Hospital, Hefei，Anhui 230001,China; 3.The Second Affiliated Hospital，Anhui University of Medical Sciences, Hefei，Anhui 230022
关键词:
心肌梗死;  动物实验; 兔; 骨髓干细胞;  细胞移植;  心功能;  二维超声
Keywords:
myocardial infarction;  animal test;  rabbit;  bone marrow stem cells;  cell transplantation;  cardiac function;  two-dimensional ultrasound
分类号:
R542.22；R540.45
DOI:
-
文献标志码:
A
摘要:
目的 应用超声评价体外不同处理方式兔骨髓间充质干细胞(MSCs)移植于缺血心肌后对心脏功能的影响。方法 采用结扎冠状动脉左前降支的方法建立心肌梗死模型，2周后分别将自体MSCs、经5-aza诱导的MSCs、骨髓单个核细胞(BMMNCs)，Dil标记后用微量注射器注射于兔缺血心肌内,并以无血清培养基注射动物为对照组。分别在术前、细胞移植前、细胞移植后2、4周之后行M型超声心动测量:左室收缩末内径(LVESD)、左室舒张末内径(LVEDD)，计算左室射血分数(LVEF)、左室短轴缩短率(LVFS)等指标。结果 细胞移植后2、4周，超声心动检查发现MSCs组和5-aza诱导的MSCs 组的兔心功能(LVEF、LVFS值)比对照组显著升高(P<0.05)，而BMMNCs组仅在移植后４周明显改善(P<0.05)；LVESD、LVEDD细胞移植组与对照组相比无显著差异(P>0.05)。结论 MSCs、经5-aza诱导的MSCs、 BMMNCs均能存活于梗死心肌中，从而改善缺血心肌后左室收缩功能。
Abstract:
Objective To investigate the roles of transplantation of bone marrow-derived mesenchymal stem cells (MSCs) on rabbit post-infarct myocardium and their influences on cardiac function. Methods The myocardial infarction model was made by using coronary artery ligation of the left anterior descending. After 2 weeks, the self pulp MSCs, 5-aza induced MSCs, and bone marrow mononuclear cells (BMMNCs) labeled with Dil were injected into ischemic myocardium of rabbits, and the injection of serum-free media used as the control group. Two-dimensional ultrasound measurements of left ventricular end-systolic diameter (LVESD) , left ventricular end-diastolic diameter (LVEDD), left ventricular ejection fraction (LVEF), left ventricular fractional shortening (LVFS) index evaluation cardiac function were carried out before operation, before and after transplantation of 2 weeks and 4 weeks. Results After implantation of 2 and 4 weeks, the myocardial function (LVEF and LVFS value) of rabbits implanted with MSCs, 5-aza induced MSCs was better than control group (P< 0.05). BMMNCs group improved after transplantation of 4 weeks (P < 0.05). No significant difference of LVESD, LVEDD was found between cells transplantation groups and control group (P > 0.05). Conclusion MSCs, 5-aza induced MSCs, BMMNCs were lived in myocardial infarction, and improved left ventricular systolic function after myocardial ischemia.

参考文献/References:

[1]Perez Millan MI, Lorenti A. Stem cells and cardiac regeneration[J]. Medicina，2006，66(6):574-582.
[2]简文豪，杨浣宜.心血管超声诊断学[M].北京:科学技术文献出版社，2006:138-139.
[3]Zhang H, Song P, Tang Y, et al. Injection of bone marrow mesenchymal stem cells in the borderline area of infracted myocardium: heart status and cell distribution[J]. J Thorac Cardiovasc Surg，2007,134(5):1321-1326.
[4] Braga LM, Rosa K, Rodrigues B, et al .Systemic delivery of adult stem cells improve cardiac function in spontaneously hypertensive rats[J]. Clin Exp Pharmacol Phsiol,2008,35(1):113-119.
[5]李树仁,齐晓勇，刘会良，等.经冠状动脉移植自体骨髓单个核细胞和间充质干细胞对急性心肌梗死模型心功能的改善[J].中国组织工程研究与临床康复，2007，11(11):2081-2086.
[6] 林 强，陈书艳.骨髓间充质干细胞冶疗急性心肌梗死的机制[J].心脏杂志，2008，20(4):498-500.
[7] Shake JG, Gruber PJ, Baumgartner WA, et al. Mesenchymal stem cell implantation in a swine myocardial infarct model: engraftment and functional effects[J]. Ann Thorac Surg,2002, 73(6): 1919-1926.
[8] Dai W, Hale SL, Martin BJ, et al. Allogeneic mesenchymal stem cell transplantation in postinfarcted rat myocardium: short and long-term effects[J]. Circulation,2005，112(2): 214-223.
[9] Gnecchi M,He HM,Liang OD,et al.Paracrine action for marked protection of ischemic heart by AKt-modified mesenchymal stem cell[J]. Nat Med,2005,11(4):367-368.

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备注/Memo

备注/Memo:
安徽省教育厅自然科学重点科研项目(2006KJ093A)
更新日期/Last Update: 2010-05-20