|本期目录/Table of Contents|

[1]过贵元,胡淑鸾,刘小朋.胰岛素对兔视网膜Müller细胞生长活性的影响[J].医学研究与战创伤救治(原医学研究生学报),2011,13(01):14-16.
　GUO Gui-yuan,HU Shu-luan,LIU Xiao-peng.Effects of insulin on the proliferation of rabbit retinal Müller cells[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2011,13(01):14-16.
点击复制

胰岛素对兔视网膜Müller细胞生长活性的影响()
分享到62.8K

《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第13卷
期数:
2011年01期
页码:
14-16
栏目:
出版日期:
2011-01-20

文章信息/Info

Title:
Effects of insulin on the proliferation of rabbit retinal Müller cells
文章编号:
1672-271X(2011)01-0014-03
作者:
过贵元; 胡淑鸾; 刘小朋
350002 福建福州，南京军区福州总医院476临床部
Author(s):
GUO Gui-yuan; HU Shu-luan; LIU Xiao-peng
Department of Ophthalmology, 476 Clinical Branch,Fuzhou General Hospital, Nanjing Military Command, Fuzhou,Fujian 350002, China
关键词:
糖尿病眼病; Müller细胞; 胰岛素; 细胞人工培养; MTT法
Keywords:
oculopathy with diabetes;  Müller cel1;  insulin;  cell culture;  MTT assay
分类号:
R587.26
DOI:
-
文献标志码:
A
摘要:
目的 通过观察不同浓度的胰岛素对视网膜Müller细胞生长活性的影响，探讨其在眼底病变中所起的作用。方法 运用组织块贴壁培养法培养兔视网膜Müller细胞，利用MTT法测定其在不同浓度胰岛素及作用时间下培养板中各孔的光吸收值。结果 当高浓度胰岛素作用时间≥3 d，光吸收(OD)值均明显下降，并随着浓度的增高及作用时间的延长而进一步减小。结论 当高浓度胰岛素作用达到一定时间时，对兔视网膜Müller细胞生长活性起着显著的抑制作用。
Abstract:
Objective To investage the role of Müller cells during the course of ocular fundus diseases by observe the effect of different concentrations of insulin on the proliferation of rabbit Müller cells. Methods Rabbit Müller cells were cultured by the explant tissue culture method. MTT assay was used to detect the OD value of 96-well plate containing different concentrations of insulin. Results The OD value was lower than that of the control group when the acting time of insulin was longer than three days, and was decreased with the increasing of insulin's concentration and acting time. Conclusion High concentration of insulin could significantly inhibit the proliferation of Müller cells when having appropriate acting time.

参考文献/References:

[1]Berweek S, Thieme H, Lepple-wiemjies A,et al. Insulin-induced hyperpolarization in retinal capillary pericytes [J］. Invest Ophthalmol Vis Sci, 1993，34 (12): 3402-3407.
[2］过贵元, 郑宏华, 陈秀丽. 正常大鼠视网膜苗勒(Müller)细胞的形态结果特点[J］. 东南国防医药, 2009, 11(3): 193-195.
[3］Gosbell AD, Favilla I, Jablonski P. The location of insulin receptors in bovine retina and isolated retinal cells [J］. Clin Exp Ophthalmol, 2002, 30 (2): 124-130.
[4］Scherer J,Schnitzer J.The rabbit retina:a suitable mammalian tissue for obtaining astroglia-free Müller cell cultures [J］. Neurosci Lett, 1989, 97(1-2): 51-56.
[5］Vinores SA,Campochiaro PA, McGehee R，et al. Ultrastructural and immunocytoche- mical changesin retinal piment epithelium, retinal glia and fibroblasts in vitreous culture [J］. Invest Ophthalmol Vis Sci, 1990, 31(21): 2529-2545.
[6］Marcus A, Bearse Jr,Anthony JA,et al. A Multifocal electroretinogram model predicting the development of diabetic retinopathy [J］. Prog Retin Eye Res, 2006,25(5): 425-448.
[7］王越晖，王心蕊，朱向红，等. 高糖高胰岛素对兔视网膜Müller细胞血管内皮生长因子表达的影响[J］. 中华糖尿病杂志, 2004,12(4): 277-280.
[8］张晓梅，孙伟英，王彬杰，等. 胰岛素对视网膜Müller细胞神经保护功能的影响[J］. 眼科新进展, 2007,27(12): 881-884.

相似文献/References:

[1]过贵元,郑宏华,陈秀丽.正常大鼠视网膜苗勒(Müller)细胞 的形态学结构特点[J].医学研究与战创伤救治(原医学研究生学报),2009,11(03):193.
　GUO Gui-yuan,ZHENG Hong-hua,CHEN Xiu-li.Morphology characteristics of Müller cells of the retina[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2009,11(01):193.

备注/Memo

备注/Memo:
南京军区医学科技创新课题(09Z036)
更新日期/Last Update: 2011-01-20