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[1]郑小花,黄惠娟.左归丸联合橄榄油对大鼠去势后骨组织和骨密度的影响[J].医学研究与战创伤救治(原医学研究生学报),2014,16(01):10-13，42.
　ZHENG Xiao-hua,HUANG Hui-juan..Effect of Zuogui pill combined with olive oil on ovariectomized rats on bone tissue and bone mineral density[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2014,16(01):10-13，42.
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左归丸联合橄榄油对大鼠去势后骨组织和骨密度的影响()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第16卷
期数:
2014年01期
页码:
10-13，42
栏目:
出版日期:
2014-02-20

文章信息/Info

Title:
Effect of Zuogui pill combined with olive oil on ovariectomized rats on bone tissue and bone mineral density
作者:
郑小花; 黄惠娟
350025 福建福州,南京军区福州总医院妇产科
Author(s):
ZHENG Xiao-hua; HUANG Hui-juan.
Department of Obstetrics and Gynecology,Fuzhou General Hospital of Nanjing Military Command,PLA,Fuzhou,Fujian 350025,China
关键词:
左归丸; 橄榄油; 骨质疏松症;  去势后大鼠
Keywords:
Zuogui pill;  olive oil;  osteoporosis;  ovariectomized rats
分类号:
R271.9;R259
DOI:
-
文献标志码:
A
摘要:
目的 观察左归丸联合橄榄油对去势后大鼠骨组织和骨密度的影响，为中医药治疗绝经后骨质疏松症提供实验依据。方法 对45只5～6月龄清洁型SD雌性大鼠进行随机等分成5组:①假手术组(Sham组)、②去卵巢组(OVX组)、③去卵巢+左归丸组(ZGW组)、④去卵巢+橄榄油组(Olive组)、⑤去卵巢+左归丸+橄榄油组(复方组)。对照组(①②)用药:均以生理盐水按1 ml/100 g体重，隔天1次灌胃。治疗组(③④⑤)用药:ZGW组:以中成药左归丸水溶液(每ml含0.2 g生药)按1 ml/100 g体重，隔天1次灌胃。Olive组:以初榨橄榄油按1 ml/100 g体重，隔天1次灌胃。复方组:以左归丸水溶液和初榨橄榄油交替每日灌胃1次。12周后分别左心室取血，检测血中血清雌二醇(E2)、白细胞介素-1(IL-1)、白细胞介素-6(IL-6)水平，放血处死后取出腰椎行双能X线骨密度测定，取左侧股骨近端1/3切片观察骨组织并计算骨小梁面积。结果 OVX组中血E2值明显低于Sham组(P<0.01)；复方组中E2、IL-1、IL-6与Sham组无差异(P>0.05)，复方组血E2值高于OVX组、ZGW组和Olive组(均P<0.05)，IL-1值低于OVX组、ZGW组和Olive组(均P<0.05)，复方组中IL-6值低于Olive组，差异有统计学意义(P<0.05)，与ZGW组相比差异无统计学意义(P>0.05)。光镜下观察发现OVX组中骨小梁稀薄、断裂，治疗组中骨小梁变密,连续性好，骨质疏松的病理骨组织现象改善，且三组间没有明显的区别。复方组骨密度较Olive组增加(P<0.05),但与ZGW组无显著差异性(P>0.05)。复方组骨小梁面积较ZGW组增加(P<0.05),但与Olive组无显著差异性(P>0.05)。结论 左归丸联合橄榄油能有效地减轻大鼠卵巢切除术引起的骨质丢失，且两者联合应用疗效或可能优于单用左归丸或橄榄油。
Abstract:
Objective Observe the effect of Zuogui pill combined with olive oil on bone metabolism and Bone Mineral Density (BMD) of ovariectomized rats,providing an experimental basis for treating postmenopausal osteoporosis with a combined treatment of diet and the traditional Chinese medicine.Methods A total of 45 healthy female Sprague-Dawley rats aging from five to six-month were divided into five groups(nine in each group): ①sham-operated control (Sham group),②ovariectomized (OVX group),both Sham group and OVX group were supplemented with normal saline,③ovariectomized rats supplemented with the aqueous solution of Zuogui pill (ZGW group),④ovariectomized rats supplemented with extra virgin olive oil (Olive group),⑤ovariectomized rats supplemented with the aqueous solution of Zuogui pill and extra virgin olive oil (compound group).12 weeks later blood samples were obtained to detect the levels of E2,IL-1 and IL-6.Then evaluate the BMD of lumbar spin and observe the structure of bone tissue of the 1/3 segment of left proximal femur slices and calculate the trabecular area.Results The compound group showed no difference in plasma E2,IL-1 and IL-6 than the Sham group (P>0.05),but a significant higher in plasma E2 levels than OVX group,ZGW group and Olive group (P<0.05) and a significant decrease in IL-1than those.The compound group also showed a significant decrease in IL-6 than Olive group (P<0.05) but no difference when compared with ZGW group (P>0.05).The trabecular was thinning and fracture in OVX group under light microscope.ZGW group,Olive group and the compound group trabecular had a turn for a better and a good continuity.The pathology phenomenon of osteoporosis improved in bone tissue and there were no significant difference between the three treated groups.The compound group showed a higher in bone mineral density compared with Olive group (P<0.05) and no difference with ZGW group (P>0.05),but a higher in trabecular area compared with ZGW group (P<0.05) and no difference with Olive group (P>0.05).Conclusion Zuogui pill combined with olive oil effectively can effectively reduce bone loss in ovariectomized rats,and the two combined effects may be better than the single use of Zuogui pill or olive oil.

参考文献/References:

[1] 曹泽毅.中华妇产科学[M]．北京:人民卫生出版社，2012:2154-2270.
[2] 吕海波，任艳玲，王 莹，等．左归丸防治去卵巢大鼠骨质疏松症的实验研究[J]．中国骨质疏松杂志，2010，11(12):847-850.
[3] 蒋天骄．中药左归丸联合维D磷酸氢钙片治疗骨质疏松症疗效观察[J]．临床合理用药杂志，2011，4(7):54.
[4] Saleh NK,Saleh HA.Olive oil effectively mitigates ovariectomy-induced osteoporosis in rats[J].BMC Complement Altern Med,2011,11(10):1-11.
[5] Hattori T,Fei W,Kizawa T,et al.The fixed herbal drug composition “Saikokaryukotsuboreito” prevents bone loss with an association of serum IL-6 reductions in ovariectomized mice model[J].Phytomedicine,2010,17(3-4):170-177.
[6] 胥少汀，葛宝丰，徐印坎．实用骨科学[M]．北京:人民军医出版社，2012:1517-1528.
[7] Czerny B,Kaminski A,Kurzawski M,et al.The association of IL-1beta,IL-2,and IL-6 gene polymorphisms with bone mineral density and osteoporosis in postmenopausal women[J].Eur J Obstet Gynecol Reprod Biol，2010，149(1):82-85.
[8] Terauchi M.Role of the immune system in the pathophysiology of postmenopausal osteoporosis[J].Nihon Rinsho,2011,69(7):1215-1219.
[9] 马丽萍，王剑火，姜绪防.碳酸钙维生素D3及鲑鱼降钙素联合脉冲电磁场治疗老年女性骨质疏松症的临床观察[J].东南国防医药，2013,15(2):154-159.
[10] 邹 军，董苗淼，张 丽，等.运动配合左归丸对去卵巢大鼠骨质疏松的偶联信号IL-1、IL-6及C0X-2传递的影响[J]．中国骨质疏松杂志，2010,16(6):407-411.
[11] 鞠大宏，吴 萍，贾红伟，等．左归丸对卵巢切除所致骨质疏松大鼠骨钙素和降钙素含量的影响[J]．中国中医药信息杂志，2003，10(1):16-17.
[12] Lewiecki EM.Phytoestrogens and their role in the management of postmenopausal osteoporosis[J].South Med J,2009,102(1):111-112.
[13] 杨利娟，黄君梅，王 飞．何首乌、骨碎补和淫羊藿的植物雌激素作用研究[J]．中药与临床,2012，3(3):37-40.
[14] 李 海，陈建海，王金花，等．山茶籽联合雌二醇对去卵巢大鼠骨组织和骨代谢生化指标的影响[J]．中国妇幼保健，2012，27(4):595-598．
[15] Puel C,Quintin A,Agalias A,et al.Olive oil and its main phenolic micronutrient (oleuropein) prevent inflammation-induced bone loss in the ovariectomized rat[J].Br J Nutr,2004,92(1):119-127.
[16] Horst-Sikorska W, Wawrzyniak A.The role of hormonal therapy in osteoporosis[J].Endokrynol Pol,2011,62(Suppl 2):19-22.
[17] Puel C,Mathey J,Agalias A,et al.Dose-response study of effect of oleuropein,an olive oil polyphenol,in an ovariectomy/inflammation experimental model of bone loss in the rat[J].Clin Nutr,2006,25(5):859-868.
[18] 金英姿，葛 亮．橄榄油的营养成分及其保健功能[J]．农产品加工(学刊)，2012，1(6):94-96.
[19] 曹 静．益心舒联合左归丸治疗绝经期综合征的临床观察[J]．中西医结合心脑血管病杂志,2012，10(8):1010-1011.

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备注/Memo

备注/Memo:
南京军区医学科技创新课题项目(11MA111)
更新日期/Last Update: 2014-01-20