[1]于 淼,原 源.HPLC法测定益心酮片中四种黄酮类成分的含量[J].医学研究与战创伤救治(原医学研究生学报),2015,17(02):157-159，165.[doi:10.3969/j.issn.1672-271X.2015.02.014]
　YU Miao,YUAN Yuan..Determination of four flavonoid ingredients in Yixintong tablets by HPLC[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2015,17(02):157-159，165.[doi:10.3969/j.issn.1672-271X.2015.02.014]
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HPLC法测定益心酮片中四种黄酮类成分的含量()

《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第17卷
期数:
2015年02期
页码:
157-159，165
栏目:
出版日期:
2015-04-22

文章信息/Info

Title:
Determination of four flavonoid ingredients in Yixintong tablets by HPLC
作者:
于 淼; 原 源
200052 上海，解放军85医院药剂科
Author(s):
YU Miao;  YUAN Yuan.
Department of Pharmacy， 85 Hospital of PLA， Shanghai 200433， China
关键词:
高效液相色谱法; 益心酮片; 牡荆素鼠李糖苷; 牡荆素葡萄糖苷; 牡荆素; 金丝桃苷
Keywords:
HPLC;  Yixintong tablets;  vitexin rhamnosides;  vitexin glucoside;  vitexin;  hyperoside
分类号:
R286
DOI:
10.3969/j.issn.1672-271X.2015.02.014
文献标志码:
A
摘要:
目的 建立高效液相色谱法（HPLC）同时测定益心酮片中牡荆素鼠李糖苷、牡荆素葡萄糖苷、牡荆素、金丝桃苷四种成分的含量。方法 采用SHISEIDO CAPCELL Pak C18 AQ 250 mm×4.6 mm色谱柱；流动相为四氢呋喃-乙酸-水-甲醇-乙腈(38∶4∶152∶3∶3)；流速为1．0 mL／min；柱温：25 ℃；检测波长：330 nm。结果 牡荆素鼠李糖苷、牡荆素葡萄糖苷、牡荆素、金丝桃苷的线性范围分别为10.11~201.02 μg/mL(r=0.9997)，7.92~158.42 μg/mL(r=0.9996)，4.02~80.42 μg/mL(r=0.9995)，6.28~125.61 μg/mL(r=0.9995)，范围内线性关系良好、精密度、重现性、回收率均符合要求。结论 该方法快速、准确、重现性好可用于本品的质量控制。
Abstract:
Objective To establish the quantitative analysis method for simultaneous determination of Vitexin rhamnosides， Vitexin glucoside， Vitexin and Hyperoside in Yixintong tablets by HPLC. Methods HPLC was performed on SHISEIDO CAPCELL Pak C18 AQ (4.6 mm×250 mm)， with the mobile phase of THF-acetic acid-water-methanol-acetonitrile (38∶4∶152∶3∶3) at a flow rate of 1.0 mL/min．The column temperature was set at 25 ℃， and the detection wavelength was 330 nm. Results The linear ranges of Vitexin rhamnoside， Vitexin glucoside， Vitexin， Hyperoside were 10.11-201.02 μg/mL (r=09997)， 7.92-158.42 μg/mL (r=0.9996)， 4.02-80.42 μg/mL (r=0.9995)， 6.28-125.61 μg/mL (r=0.9995) respectively. The linear relationship， precision， reproducibility， recovery rates all meet the requirements. Conclusion The content of Vitexin rhamnosides is significantly higher than Hyperoside， and the study shows that Vitexin rhamnosides has effect on dilating blood vessels， protecting cardiac tissue， which is consistent with the effect of the Yixintong tablets. Therefore， it's not reasonable to choose Hyperoside as the only quality control indicator of the Yixintong tablets in the 2010 Chinese Pharmacopoeia. Vitexin rhamnoside and Vitexin glucoside are also important pharmacological active components in Hawthorn Leaves， and could be considered as quality control indicators of the Yixintong tablets.

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2015-03-20