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[1]李锐成,刘昕阳,沈建军,等.T-SPOT.TB联合ADA和TB-DNA在诊断结核性胸膜炎中的应用价值[J].医学研究与战创伤救治(原医学研究生学报),2016,18(05):483-485,504.[doi:10.3969/j.issn.1672-271X.2016.05.010]
　LI Rui-cheng,LIU Xin-yang,SHEN Jian-jun,et al.Application value of T-SPOT.TB combined with ADA and bacillus tuberculosis DNA in the diagnosis of tuberculous pleurisy[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2016,18(05):483-485,504.[doi:10.3969/j.issn.1672-271X.2016.05.010]
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T-SPOT.TB联合ADA和TB-DNA在诊断结核性胸膜炎中的应用价值()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第18卷
期数:
2016年05期
页码:
483-485,504
栏目:
出版日期:
2016-09-20

文章信息/Info

Title:
Application value of T-SPOT.TB combined with ADA and bacillus tuberculosis DNA in the diagnosis of tuberculous pleurisy
作者:
李锐成; 刘昕阳; 沈建军; 邹菊贤; 张惠中
710038陕西西安，第四军医大学唐都医院检验科
Author(s):
LI Rui-cheng;  LIU Xin-yang;  SHEN Jian-jun;  ZOU Ju-xian;  ZHANG Hui-zhong
Department of Laboratory, Tangdu Hospital,Forth Military Medical University,Xi’an，Shaanxi 710038, China
关键词:
胸腔积液; 腺苷脱氨酶; 结核感染T细胞斑点试验; 结核性胸膜炎; 临床诊断
Keywords:
pleural effusion;  adenosine deaminas;  T cell spot test of TB infection;  tuberculous pleurisy;  clinical diagnosis
分类号:
R378
DOI:
10.3969/j.issn.1672-271X.2016.05.010
文献标志码:
A
摘要:
目的 探讨结核感染T细胞斑点试验(T-SPOT.TB)技术联合胸水腺苷脱氨酶（ADA）和痰液结核杆菌（TB-DNA）测定对结核性胸膜炎的定性诊断，以达到早期诊断的目的。方法 选取唐都医院2014年7月-2015年12月收治的86例结核性胸膜炎患者作为试验组，80例非结核性胸膜炎患者作为对照组，均行T-SPOT.TB检测，同时联合检查患者胸水ADA和痰液TB-DNA，比较T-SPOT.TB、ADA和TB-DNA检测与三者联合检测的应用价值。结果 86例结核性胸膜炎患者，T-SPOT.TB阳性率为84.88%(73/86)，ADA阳性率为53.48%(46/86)，痰液TB-DNA阳性率为31.40% (27/86)，三者联合检测阳性率为93.02%(80/86)。结论 T-SPOT.TB检测联合ADA和痰液TB-DNA检测对结核性胸膜炎的诊断有较高的敏感性,对疑似结核性胸膜炎患者的快速、准确诊断具有重要的辅助诊断价值。
Abstract:
Objective To discuss the qualitative diagnosis of tuberculous pleurisy under T cell spot test of TB infection(T-SPOT.TB) combined with ADA and real-time fluorescent quantity PCR or detection of bacillus tuberculosis DNA(TB-DNA) to achieve early diagnosis and treatment purpose. Methods We selected 86 patients of tuberculous pleurisy as the observation group and 80 patients of non-tuberculous pleurisy as the control group received treatment in our hospital from July 2014 to December 2015, and gave them T-SPOT.TB detection and at the same time, did the joint inspection of ADA and TB-DNA levels and compared with the Results of the combined determination of three administered simultaneously to evaluate the clinical value. Results Positive rate of T-SPOT.TB, ADA, sputum TB-DNA determination and the combined determination of three for 86 patients with tuberculous pleurisy was 84.88% (73/86), 53.48% (46/86), 31.40% (27/86) and 93.02% (80/86) respectively. Conclusion The combined detection of T-SPOT.TB, ADA and sputum TB-DNA has a higher sensitivity in diagnosis of tuberculous pleurisy, and has important auxiliary diagnostic value for patients with suspected tuberculous pleurisy.

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2016-12-09