|本期目录/Table of Contents|

[1]龚谋春,方晓明,姚宁,等.幽门螺杆菌相关性胃腺癌中IL-37的表达及意义[J].医学研究与战创伤救治(原医学研究生学报),2017,19(02):153-156.[doi:10.3969/j.issn.1672-271X.2017.02.010]
　GONG Mou-chun,FANG Xiao-ming,YAO Ning,et al.IL-37 level in HP positive patients with gastric adenocarcinoma[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2017,19(02):153-156.[doi:10.3969/j.issn.1672-271X.2017.02.010]
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幽门螺杆菌相关性胃腺癌中IL-37的表达及意义()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第19卷
期数:
2017年02期
页码:
153-156
栏目:
出版日期:
2017-03-20

文章信息/Info

Title:
IL-37 level in HP positive patients with gastric adenocarcinoma
作者:
龚谋春; 方晓明; 姚宁; 刘慧慧; 陈琪枫; 刘桂宝
310004杭州，解放军第117医院普外科
Author(s):
GONG Mou-chun;  FANG Xiao-ming;  YAO Ning;  LIU Hui-hui;  CHEN Qi-feng;  LIU Gui-bao
（Department of General Surgery, the 117th Hospital of PLA，Hangzhou 310004，Zhejiang，China）
关键词:
幽门螺杆菌; 胃腺癌; 白细胞介素37
Keywords:
Helicobacter pylori;  Gastric adenocarcinoma;  Interleukin-37
分类号:
R735.2
DOI:
10.3969/j.issn.1672-271X.2017.02.010
文献标志码:
A
摘要:
目的 探讨白细胞介素37（IL-37）在幽门螺杆菌（HP）相关性胃腺癌[HP（+）胃腺癌]中的表达及意义。方法 选取2014年1月至2015年12月解放军第117医院收治胃腺癌78例（HP+ 46例、HP- 32例），同期门诊HP（+）胃溃疡及HP（-）正常患者各30例。采用酶联免疫吸附法（ELISA）定量检测各组血浆IL-37蛋白表达；Western blot检测HP（+）和HP（-）胃腺癌组癌组织内IL-37蛋白表达。逆转录（RT）-PCR检测HP（+）与HP（-）胃腺癌癌组织IL-37 mRNA表达。结果 ①各组血浆IL-37蛋白表达情况：HP（-）正常组与HP（-）胃腺癌组相比，差异无统计学意义（P>0.01）；HP（+）溃疡组高于HP（-）正常组和HP（-）胃腺癌组，差异均有统计学意义（P<0.01）；HP（+）胃腺癌组高于HP（+）溃疡组、HP（-）正常组及HP（-）胃腺癌组，差异均有统计学意义（P<0.01）。②胃腺癌组癌组织内IL-37蛋白表达情况：HP（+）组高于HP（-）组，差异有统计学意义。③胃腺癌组癌组织内IL-37 mRNA表达情况：HP（+）组高于HP（-）组，差异有统计学意义（P<0.01）。结论 HP感染后血浆IL-37升高，HP（+）胃癌组织内IL-37蛋白及mRNA表达均升高；IL-37表达升高与HP（+）胃腺癌的发生密切相关。
Abstract:
Objective To investigate the expression of interleukin-37 in Helicobacter pylori(HP) positive patients with gastric adenocarcinoma.Methods 78 cases of patients with gastric adenocarcinoma recruited in our hospital from January 2014 to December 2015 were selected. Among them, seventy-eight inpatients with gastric adenocarcinoma [HP(+) 46 cases, HP(-) 32 cases], and 30 outpatients with HP(+) gastric ulcer, and 30 outpatients with HP(-) were studied. The plasma IL-37 levels of each group were determined by ELISA. Western blot was used to test IL-37 protein in cancer tissue of HP(+) and HP(-) cases with gastric adenocarcinoma. And RT-PCR was used to test IL-37 mRNA in cancer tissue of HP(+) and HP(-) cases with gastric adenocarcinoma tissue.Results ① Expression of plasma IL-37 protein in each group: there were no statistically significant difference between HP(-) group and HP(-) cases with gastric adenocarcinoma (P >0.01). It was increased signifcantly in the HP(+) ulcer group compared with HP(-) group and HP(-) cases with gastric adenocarcinoma (P<0.01), and it was increased signifcantly in HP(+) cases with gastric adenocarcinoma compared with other groups (P<0.01). ② Expression of IL-37 protien in gastric adenocarcinoma tissue: it was increased signifcantly in HP(+) cases with gastric adenocarcinoma compared with HP(-) cases with gastric adenocarcinoma (P<0.01). ③ Expression of IL-37 mRNA level in gastric adenocarcinoma tissue: it was increased signifcantly in HP(+) cases with gastric adenocarcinoma compared with HP(-) cases with gastric adenocarcinoma (P<0.01).Conclusion The plasma IL-37 level was increased when patients were infected by HP, and the expression of IL-37 protein and mRNA were increased significantly in gastric adenocarcinoma tissue of HP (+) patient with gastric adenocarcinoma. IL-37 might involved in the occurrence and development of gastric adenocarcinoma in patients with HP.

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2017-03-20