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[1]谢荣臻,曾祥福,邓伟,等.miR-10a在胰腺癌侵袭转移中的作用[J].医学研究与战创伤救治(原医学研究生学报),2017,19(04):352-356.[doi:10.3969/j.issn.1672-271X.2017.04.005]
　XIE Rong-zhen,ZENG Xiang-fu,DENG Wei,et al.The role of miR-10a in invasion and metastasis of pancreatic cancer[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2017,19(04):352-356.[doi:10.3969/j.issn.1672-271X.2017.04.005]
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miR-10a在胰腺癌侵袭转移中的作用()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第19卷
期数:
2017年04期
页码:
352-356
栏目:
出版日期:
2017-08-10

文章信息/Info

Title:
The role of miR-10a in invasion and metastasis of pancreatic cancer
作者:
谢荣臻; 曾祥福; 邓伟; 刘晓平; 章新华
作者单位：341000赣州，赣南医学院第一附属医院普外二科
Author(s):
XIE Rong-zhen; ZENG Xiang-fu; DENG Wei; LIU Xiao-ping; ZHANG Xin-hua
(Department of General Surgery，the First Affiliated Hospital of Gannan Medical University，Ganzhou 341000，Jiangxi，China)
关键词:
miR-10a; 胰腺癌; 侵袭; 迁移; 临床病理特征; 转染
Keywords:
miR-10a;  Pancreatic cancer;  Invasion;  Migration;  Clinic opathological features;  Transfection
分类号:
R735.9
DOI:
10.3969/j.issn.1672-271X.2017.04.005
文献标志码:
A
摘要:
目的 探讨microRNA-10a（miR-10a）在胰腺癌侵袭转移中的作用。方法 选取2014年1月至2016年12月在赣南医学院第一附属医院切除的胰腺癌组织标本68例，同时选取癌旁组织作为对照，采用逆转录-聚合酶链反应(RT-PCR)检测组织标本miR-10a表达；同时选取胰腺癌细胞株Capan-1细胞，以脂质体转染法将miR-10a重组正、反义真核表达质粒载体转染Capan-1细胞，采用RT-PCR检测转染细胞miR-10a表达，Transwell和Matrigel试验观察转染细胞的迁移和侵袭能力，四甲基偶氮唑盐(MTT)法检测细胞增殖能力。结果 胰腺癌组织miR-10a相对表达量为（0.213±0.024），明显高于癌旁组织（P＜0.01）；中低分化胰腺癌miR-10a相对表达量为（0.212±0.011），明显高于高分化胰腺癌（P＜0.01）；有淋巴结转移胰腺癌miR-10a相对表达量为（0.217±0.017），明显高于无淋巴结转移胰腺癌（P＜0.05）；TNM分期为Ⅲ～Ⅳ期胰腺癌miR-10a相对表达量为（0.230±0.015），明显高于Ⅰ～Ⅱ期胰腺癌（P＜0.01）。转染48 h后，正义组miR-10a相对表达量为（0.021±0.010），明显高于反义组、空载组和空白对照组（P＜0.05）；反义组miR-10a相对表达量为（0.003±0.001），明显低于空载组和空白对照组（P＜0.05）。正义组24 h和48 h 光密度（OD）值分别为（0.602±0.018）和（0.753±0.041），明显高于反义组、空载组和空白对照组（P＜0.05）；反义组24 h和48 h OD值分别为（0.451±0.023）和（0.591±0.038），明显低于空载组和空白对照组（P＜0.05）。正义组迁移和侵袭细胞数分别为（85.22±12.10）个和（140.24±30.54）个，明显高于反义组、空载组和空白对照组（P＜0.05）；反义组迁移和侵袭细胞数分别为（12.52±3.22）个和（20.41±8.66）个，明显低于空载组和空白对照组（P＜0.05）。结论 miR-10a过表达可对胰腺癌有生长促进、增强侵袭和迁移的作用，值得进一步研究。
Abstract:
Objective To investigate the role of miR-10a in invasion and metastasis of pancreatic cancer.Methods Selected 68 cases of pancreatic cancer tissue in our hospital from January 2014 to December 2016, the adjacent tissues were selected as control, MiR-10a expression in tissue specimen was detected by RT-PCR; selected pancreatic cancer cell line Capan-1 cell, used liposome transfection method, MiR-10a recombinant positive and antisense eukaryotic expression vector was transfected into Capan-1 cells, the expression of miR-10a in transfected cells was detected by RT-PCR, and the migration and invasion ability of transfected cells were observed by Transwell and Matrigel assay, the proliferation of cells was detected by MTT assay.Results The relative expression of miR-10a in pancreatic cancer tissues was (0.213±0.024), which was significantly higher than that in adjacent tissues (P<0.01); The relative expression of miR-10a in intermediate low differentiated pancreatic cancer was (0.212±0.011), which was significantly higher than that of high differentiated pancreatic cancer (P<0.01); The relative expression of miR-10a in lymph node metastasis of pancreatic cancer was (0.217±0.017), which was significantly higher than that without lymph node metastasis (P<0.05); The relative expression level of miR-10a in TNM stage III to IV pancreatic cancer was (0.230±0.015), which was significantly higher than that of stage I to II pancreatic cancer (P<0.01). After transfection of 48 h, the relative expression of miR-10a in positive group (0.021±0.010), was significantly higher than that of antisense group, empty vector group and blank control group (P<0.05); antisense miR-10a group relative expression was (0.003±0.001), significantly lower than the empty vector group and blank control group (P<0.05). Positive group 24 h and 48 h OD values were (0.602±0.018) and (0.753±0.041), were significantly higher than that of antisense group, empty vector group and blank control group (P<0.05); Antisense group 24 h and 48 h OD values were (0.451±0.023) and (0.591±0.038), and were significantly lower than that of empty vector group the blank control group (P<0.05). Positive group cell migration and invasion were (85.22±12.10) and (140.24±30.54), were significantly higher than that of antisense group, empty vector group and blank control group (P<0.05); antisense group cell migration and invasion were (12.52±3.22) and (20.41±8.66), were significantly higher than that of empty vector group and blank control group (P<0.05).Conclusion Overexpression of miR-10a can promote the growth and enhance the invasion and migration of pancreatic cancer, which is worthy of further study.

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备注/Memo

备注/Memo:
基金项目：赣州市指导性科技计划项目（GZ2015ZSF100）
更新日期/Last Update: 2017-07-20