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[1]陈桂莲,黄惠娟,田黎明.橄榄苦甙对卵巢辐射损伤的防治效果[J].医学研究与战创伤救治(原医学研究生学报),2017,19(06):576-580.[doi:10.3969/j.issn.1672-271X.2017.06.005]
 CHEN Gui-lian,HUANG Hui-juan,TIAN Li-ming.Experimental study of the effects ofoleuropein on irradiation-induced ovarian injury[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2017,19(06):576-580.[doi:10.3969/j.issn.1672-271X.2017.06.005]
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橄榄苦甙对卵巢辐射损伤的防治效果()

《医学研究与战创伤救治》(原医学研究生学报)[ISSN:1672-271X/CN:32-1713/R]

卷:
第19卷
期数:
2017年06期
页码:
576-580
栏目:
出版日期:
2017-11-23

文章信息/Info

Title:
Experimental study of the effects ofoleuropein on irradiation-induced ovarian injury
作者:
陈桂莲1黄惠娟1田黎明2
作者单位:1. 350025福州,福建医科大学福总临床医学院(南京军区福州总医院)妇产科;2. 350025福州,厦门大学附属东方医学院妇产科
Author(s):
CHEN Gui-lian1 HUANG Hui-juan1 TIAN Li-ming2
(1. Department of Obestetrics and Gynecology,Clinical Medical School, Fuzhou General Hospital of Fujian Medical University/Fuzhou General Hospital of Chinese PLA, Fuzhou 350025,Fujian,China; 2.Department of Obstetrics and Gynecology,East Hospital Affiliated to Xiamen University,Fuzhou 350025,Fujian,China)
关键词:
橄榄苦甙电离辐射卵巢储备功能炎症因子
Keywords:
Oleuropein Irradiation Ovarian reserve Cytokines
分类号:
R711.4
DOI:
10.3969/j.issn.1672-271X.2017.06.005
文献标志码:
A
摘要:
目的探讨橄榄苦甙减轻卵巢辐射损伤的效果及其机制。方法32只成熟雌性SD大鼠随机分为4组,正常对照组和辐射组予蒸馏水10mL/(kg·d),橄榄苦甙组和辐射+橄榄苦甙组予橄榄苦甙100mg/(kg·d),连续灌喂12周。12周后,辐射组和辐射+橄榄苦甙组大鼠动情期时,接受5 Gy的X线盆腔照射1次。辐射后7d检测血清抗苗勒氏管激素(AMH)、IL-1、IL-6和TNF-α含量,计算卵巢组织各级卵泡数。结果与正常对照组比较,辐射组血清AMH浓度显著降低(P<0.05),血清IL-1、IL-6、TNF-α浓度升高(P<0.05);卵巢中卵泡数减少。与辐射组比较,辐射+橄榄苦甙组血清AMH升高,IL-1、IL-6、TNF-α浓度降低(P<0.05);卵泡总数增加。结论橄榄苦甙可通过抑制辐射诱导的炎症反应,减轻卵巢的辐射性损伤。
Abstract:
ObjectiveTo study the effects of oleuropein on irradiation-induced ovarian injury.Methods32 femal SD rats were randomly assigned to 4 groups of 8 rats each: control group, oleuropein group, irradiation group and irradiation + oleuropein group. Rats in control group and irradiation group received distilled water 10 mL/(kg·d) intragastrically. Rats in oleuropein group and irradiation + oleuropein group received oleuropein 100 mg/(kg·d) intragastrically. 12 weeks later, rats in group irradiation group and irradiation + oleuropein group were exposed to pelvic irradiation at a dose of 5 Gy when they were in the estrus. After 7 days for radiation, the concentration of AMH, IL-1, IL-6 and TNF-α were detected and the number of growing follicles in ovarian tissue were counted.ResultsCompared with the control group, the serum AMH concentration in the radiation group was significantly decreased (P<0.05), and IL-1, IL-6 and TNF-α were increased (P<0.05) and the number of follicles in the ovaries was reduced. Compared with the radiation group, the serum AMH concentration were increased in the radiation + peridol group, and IL-1, IL-6 and TNF-α decreased (P<0.05). The total number of follicle was increased.ConclusionIt was concluded that oleuropein could inhibit the radiation-induced inflammatory response to reduce the radiation damage of the ovaries.

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备注/Memo

备注/Memo:
基金项目:福建省科技计划项目(2014Y5008);全军医药卫生科研项目(14MS129)
更新日期/Last Update: 2017-11-20