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[1]沈俊逸,赵智明,刘春丽,等.人参皂苷Rg1纳米颗粒的构建、表征和体外功能研究[J].医学研究与战创伤救治(原医学研究生学报),2019,21(01):1-6.[doi:10.3969/j.issn.1672-271X.2019.01.001]
　SHEN Jun-yi,ZHAO Zhi-ming,LIU Chun-li,et al.Construction and characterization of ginsenoside Rg1 nanoparticles and its in vitro activity[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2019,21(01):1-6.[doi:10.3969/j.issn.1672-271X.2019.01.001]
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人参皂苷Rg1纳米颗粒的构建、表征和体外功能研究()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第21卷
期数:
2019年01期
页码:
1-6
栏目:
基础研究
出版日期:
2019-01-20

文章信息/Info

Title:
Construction and characterization of ginsenoside Rg1 nanoparticles and its in vitro activity
作者:
沈俊逸; 赵智明; 刘春丽; 壮雨雯; 徐文俊; 蔡辉
作者单位：210002南京，东部战区总医院中西医结合科（沈俊逸、赵智明、刘春丽、壮雨雯、徐文俊、蔡辉）
Author(s):
SHEN Jun-yi; ZHAO Zhi-ming; LIU Chun-li; ZHUANG Yu-wen; XU Wen-jun; CAI Hui
(Department of Integrated of Traditional Chinese and Western Medicine, General Hospital of Eastern Theater Command，PLA，Nangjing 210002，Jiangsu，China)
关键词:
脑梗死; 人参皂苷Rg1; 血脑屏障; 转铁蛋白受体
Keywords:
cerebral infarction;  ginsenoside Rg1 nanoparticle; blood brain barrier;  transferrin receptor
分类号:
R743
DOI:
10.3969/j.issn.1672-271X.2019.01.001
文献标志码:
A
摘要:
目的 制备装载人参皂苷Ginsenoside Rg1（Rg1）的纳米药物载体，并进行纳米药物载体的粒径、分散度和Zeta电势的表征，检测药物体外缓释效果，并检测对脑血管内皮体外功能的影响。方法 载药系统以聚谷氨酸为骨架，包裹单体Rg1，同时偶联了转铁蛋白受体的单克隆抗体（OX26），制备载药系统（PHRO）。利用动态纳米粒度仪检测载药系统的纳米尺寸、分散系数和Zeta电势。透射电镜检测纳米载药系统的形态和尺寸。体外透析的方法 检测Rg1从纳米载药系统中的缓释效果。体外利用Trans-well实验检测PHRO对内皮细胞迁移能力的影响，利用流式细胞术检测PHRO的靶向功能，利用高效液相层析仪检测PHRO通过体外血脑屏障的效果。结果 动态纳米粒度仪显示PHRO的平均尺寸是（79±18）nm，分散系数（PDI）是0.18。 透射电镜显示PHRO是椭圆形的其半径是（89±23）nm，同时PHRO还具有良好的体外缓释功能。PHRO能明显的促进内皮细胞的迁移能力，实现对血管内皮细胞的靶向功能，同时能穿越体外血脑屏障模型。结论 纳米载药系统（PHRO）具有良好的纳米尺寸，分散度比较小尺寸均一，具备良好的缓释效果，能显著促进内皮细胞迁移，具备穿越体外血脑屏障的潜力。
Abstract:
Objective The nanoparticle drug carrier that crosses the blood-brain barrier and loaded with Ginsenoside Rg1 (Rg1) was prepared. The nanoparticle size and Zeta potential were characterized. The in vitro drug release and the effect on the migration ability of cerebral vascular endothelial cells were detected.Methods A drug delivery system (PHRO) was prepared. The drug delivery system was based on polyglutamic acid as the skeleton, wrapping the monomer Rg1, and coupled with transferrin receptor the monoclonal antibody (OX26). The nano size, dispersion coefficient and Zeta potential of the drug loading system were detected by dynamic nanoparticle size analyzer. The morphology and size of nanoscale drug loading system were detected by transmission electron microscopy. The method of in vitro dialysis was used to detect the release effect of Rg1 from nanoscale drug delivery system. The effect of PHRO on the mobility of cerebrovascular endothelial cells (CECs) was detected by Trans-well assay, CECs target function of PHRO was detected by flow cytometry and the effect of PHRO through the blood brain barrier was detected by high performance liquid chromatography.Results Dynamic light scattering(DLS) show the average particle size of PHRO was （79±18）nm and the polydispersity index (PDI)=0.18. The transmission electron microscope(TEM) images showed that all nanoparticles were spherical with diameters of （89±23）nm. PHRO was with sustained release manner and could promote the migration，realizes CECs targeting and pass through the blood brain barrier in vitro.Conclusion The nano drug delivery system (PHRO) has good nano size, small dispersion and uniform size. It has excellent sustained release effect and can significantly promote endothelial cell migration.

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金(81803943)
更新日期/Last Update: 2019-01-20