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[1]陈立慧,李璐璐.茶多酚对H9C2大鼠心肌细胞缺氧/复氧损伤的保护作用机制[J].医学研究与战创伤救治(原医学研究生学报),2020,22(3):228-233.[doi:10.3969/j.issn.1672-271X.2020.03.002]
　CHEN Li-hui,LI Lu-lu.Study on the protective effect and mechanism of tea polyphenols on H9C2 rat cardiomyocytes injury induced by hypoxia/reoxygenation[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2020,22(3):228-233.[doi:10.3969/j.issn.1672-271X.2020.03.002]
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茶多酚对H9C2大鼠心肌细胞缺氧/复氧损伤的保护作用机制()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第22卷
期数:
2020年3期
页码:
228-233
栏目:
基础研究
出版日期:
2020-05-15

文章信息/Info

Title:
Study on the protective effect and mechanism of tea polyphenols on H9C2 rat cardiomyocytes injury induced by hypoxia/reoxygenation
作者:
陈立慧; 李璐璐
作者单位：210002南京，东部战区空军医院高干科（陈立慧），空勤科（李璐璐）
Author(s):
CHEN Li-hui1;  LI Lu-lu2
(1.Cadre Ward,2. Aircrew Section, Air Force Hospital of Eastern Theater, Nanjing 210002, Jiangsu,China)
关键词:
缺氧/复氧损伤; 大鼠; 心肌细胞; 茶多酚; 氧化应激; 炎症因子; 信号通路
Keywords:
hypoxia/reoxygenation injury;  rat;  cardiomyocytes;  tea polyphenols;  oxidative stress;  inflammatory factors;  signaling pathways
分类号:
R320.2410
DOI:
10.3969/j.issn.1672-271X.2020.03.002
文献标志码:
A
摘要:
目的探究茶多酚对H9C2大鼠心肌细胞缺氧/复氧损伤的保护作用及机制。方法实验开展时间为2017年12月至2019年3月，分为对照组、缺氧/复氧组、低浓度茶多酚组和高浓度茶多酚组，对照组予以常规孵箱培养H9C2大鼠心肌细胞，缺氧/复氧组利用缺氧孵箱和常规孵箱交替模拟缺血再灌注环境培养H9C2大鼠心肌细胞，低浓度茶多酚组在缺氧孵箱培养后在常规孵箱培养前应用2 mg/mL的茶多酚，高浓度茶多酚组加入4 mg/mL的茶多酚。利用CCK-8法检测48 h培养后各组细胞活性；利用试剂盒检测48 h培养后各组丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)等氧化还原指标和髓过氧化物酶(MPO)、白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)和IL-6等炎症指标；利用免疫印迹技术检测丝裂原活化蛋白激酶(MAPK)和核因子κB通路相关分子。结果与缺氧/复氧组相比，茶多酚组细胞活性增强（P<0.05）；与缺氧/复氧组相比，茶多酚组MDA含量减少（P<0.05），SOD、CAT和GSH-Px含量增加（P<0.05）；与缺氧/复氧组相比，茶多酚组MPO、IL-1β、TNF-α和IL-6含量减少（P<0.05）；与缺氧/复氧组相比，茶多酚组MAPK和核因子κB通路相关分子表达被抑制（P<0.05）。结论茶多酚能够通过丝裂原活化蛋白激酶和核因子κB通路对H9C2大鼠心肌细胞缺氧/复氧损伤发挥保护作用。
Abstract:
ObjectiveTo examine the protective effect and mechanism of tea polyphenols on H9C2 rat cardiomyocytes injury induced by hypoxia/reoxygenation.MethodsThe experiments were conducted from December 2017 to March 2019. H9C2 rat cardiomyocytes were randomly divided into control group, hypoxia/reoxygenation group, low concentrations of tea polyphenols group and high concentrations of tea polyphenols group. H9C2 rat cardiomyocytes in the control group were cultured in conventional incubator, and cells in the hypoxia/reoxygenation group were cultured in hypoxia incubator and conventional incubator alternatively to simulate ischemia reperfusion injury of skeletal muscle cells. Cells in the low and high concentrations of tea polyphenols groups were treated with 2 mg/mL and 4 mg/mL tea polyphenols after incubation in hypoxic incubator and before incubation in conventional incubator. CCK-8 assay was used to test cell viability. Indicated test kits were used to examine the content of redox indexes such as malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) and inflammatory indexes such as myeloperoxidase (MPO), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). Western blot was used to detect the expression of mitogen activated protein kinase (MAPK) and nuclear factor-κB pathway related molecules.ResultsCompared with the hypoxia/reoxygenation group, the viability of H9C2 rat cardiomyocytes was increased in tea polyphenols group (P<0.05). Compared with the hypoxia/reoxygenation group, the content of malondialdehyde was decreased, while the content of superoxide dismutase, catalase and glutathione peroxidase were increased in the tea polyphenol group (P<0.05). Compared with the hypoxia/reoxygenation group, the contents of myeloperoxidase, IL-1β, TNF-α and IL-6 were decreased in the tea polyphenol group (P<0.05). Compared with the hypoxia/reoxygenation group, the expression of mitogen activated protein kinase and nuclear factor-κB pathway related molecules in tea polyphenol group was inhibited (P<0.05).ConclusionTea polyphenols protect H9C2 rat cardiomyocytes from hypoxia/reoxygenation injury via mitogen activated protein kinase and nuclear factor-κB pathway.

参考文献/References:

[1]吕磊, 张洁, 殷宇刚，等. 川芎嗪减轻大鼠心肌缺血再灌注损伤后心肌细胞凋亡及其机制研究[J]. 东南国防医药, 2016, 18(4): 361-364，367.
[2]Liu NB, Wu M, Chen C, et al. Novel molecular targets participating in myocardial ischemia-reperfusion injury and cardioprotection[J]. Cardiol Res Pract, 2019, 2019: 6935147.doi: 10.1155/2019/6935147.
[3]Khan N, Mukhtar H. Tea polyphenols in promotion of human health[J]. Nutrients, 2018, 11(1): E39.
[4]Mao X, Gu C, Chen D, et al. Oxidative stress-induced diseases and tea polyphenols[J]. Oncotarget, 2017, 8(46): 81649-81661.
[5]Afzal M, Safer AM, Menon M. Green tea polyphenols and their potential role in health and disease[J]. Inflammopharmacology, 2015, 23(4): 151-161.
[6]聂佩, 孟凡静, 张金国,等. 黄芪甲苷抑制血管紧张素Ⅱ诱导的心肌H9c2细胞凋亡[J]. 中国病理生理杂志, 2019, 35(11): 1942-1950.
[7]Song Y, Li X, Gong X, et al. Green tea polyphenols improve isoflurane-induced cognitive impairment via modulating oxidative stress[J]. J Nutr Biochem, 2019, 73: 108213.
[8]徐姗,胡楠.铁皮石斛多糖对H9C2大鼠心肌细胞缺血再灌注损伤的保护作用[J].郑州大学学报(医学版), 2019, 54(5): 766-769.
[9]李锋, 刘晶, 张成成，等. 卡维地洛缓解心肌细胞缺血再灌注损伤的作用机制[J]. 医学研究生学报, 2018, 31(8): 800-806.
[10]赵伟, 王永伟, 韦冠山，等. PARP-1介导的自噬流受阻在大鼠心肌细胞缺血再灌注损伤中的的作用[J]. 南方医科大学学报, 2018, 38(8): 975-979.
[11]Sun Z, Cao X, Hu Z, et al. MiR-103 inhibits osteoblasts proliferation mainly through decreasing expression of Cav1.2 in simulated microgravity[J]. Bone, 2015, 76(3): 121-128.
[12]Gomes RFA, Mitrev YN, Simeonov SP. Going beyond the limits of the biorenewable platform: sodium dithionite-promoted stabilization of 5-Hydroxymethylfurfural[J]. Chem Sus Chem, 2018, 11(10): 1612-1616.
[13]Neyt NC, Riley DL. Mild and selective reduction of aldehydes utilising sodium dithionite under flow conditions[J]. Beilstein J Org Chem, 2018, 14(4):1529-1536.
[14]Wang R, Wang M, Zhou J, et al. Shuxuening injection protects against myocardial ischemia-reperfusion injury through reducing oxidative stress, inflammation and thrombosis[J]. Ann Transl Med, 2019, 7(20): 562.
[15]Liu S, He Y, Shi J, et al. Allicin attenuates myocardial ischemia reperfusion injury in rats by inhibition of inflammation and oxidative stress[J]. Transplant Proc, 2019, 51(6): 2060-2065.
[16]Wang R, Yang Z, Zhang J, et al. Liver injury induced by carbon tetrachloride in mice is prevented by the antioxidant capacity of Anji White Tea Polyphenols[J]. Antioxidants, 2019, 8(3): E64.
[17]Qian Y, Zhang J, Fu X, et al. Preventive effect of raw Liubao Tea Polyphenols on mouse gastric injuries induced by HCl/Ethanol via anti-oxidative stress[J]. Molecules, 2018, 23(11): E2848.
[18]Cong L, Cao C, Cheng Y,et al. Green tea polyphenols attenuated glutamate excitotoxicity via antioxidative and antiapoptotic pathway in the primary cultured cortical neurons[J]. Oxid Med Cell Longev, 2016, 2016(2): 2050435.
[19]潘妍霓, 赵欣, 龙兴瑶,等. 大叶苦丁茶多酚对四氯化碳致小鼠肝损伤的预防作用[J]. 食品工业科技, 2019, 40(9):287-294.
[20]沈海涛, 吴娜, 赵宏宇,等. 茶多酚对百草枯中毒大鼠肾脏氧化应激及炎症反应的影响[J]. 中国医科大学学报,2017, 46(3): 210-213,218.
[21]王晓芹, 邓小燕, 于晓斌,等. 茶多酚通过降脂、抗炎、抗氧化以及调控TGF-β/Smad信号通路缓解2型糖尿病[J]. 中药药理与临床, 2018, 34(3): 46-50.
[22]Xiang Y, Ye S, Cai C, et al. Salvianolic acid a attenuates limb ischemia/reperfusion injury in skeletal muscle of rats[J]. Biomed Pharmacother, 2018, 97: 551-556.
[23]Zhu N, Cai C, Zhou A, et al. Schisandrin B Prevents Hind Limb from Ischemia-Reperfusion-Induced Oxidative Stress and Inflammation via MAPK/NF-κB Pathways in Rats[J]. Biomed Res Int, 2017, 2017: 4237973. doi: 10.1155/2017/4237973.

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备注/Memo:
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更新日期/Last Update: 2020-05-15