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[1]朱成宾,潘玉琴,何帮顺.微小RNA多态性与中国女性人群乳腺癌发病及病理特征的相关性研究[J].医学研究与战创伤救治(原医学研究生学报),2015,17(01):22-25.[doi:10.3969/j.issn.1672-271X.2015.01.007]
　ZHU Cheng-bin,PAN Yu-qin,HE Bang-shun..Study on the association of polymorphisms in microRNAs and risk of breast cancer and its clinic pathological parameters[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2015,17(01):22-25.[doi:10.3969/j.issn.1672-271X.2015.01.007]
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微小RNA多态性与中国女性人群乳腺癌发病及病理特征的相关性研究()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第17卷
期数:
2015年01期
页码:
22-25
栏目:
出版日期:
2015-01-31

文章信息/Info

Title:
Study on the association of polymorphisms in microRNAs and risk of breast cancer and its clinic pathological parameters
作者:
朱成宾1; 潘玉琴2; 何帮顺2
210006 江苏南京，南京医科大学附属南京医院(南京市第一医院)，1.检验科，2.中心实验室
Author(s):
ZHU Cheng-bin1;  PAN Yu-qin2;  HE Bang-shun2.
1．Department of Clinical Laboratory， 2．Central Laboratory， Nanjing First Hospital， Nanjing Medical University， Nanjing， Jiangsu 210006， China
关键词:
乳腺癌; 微小核糖核酸; 多态性
Keywords:
breast cancer;  microRNA;  polymorphism
分类号:
R737.9
DOI:
10.3969/j.issn.1672-271X.2015.01.007
文献标志码:
A
摘要:
目的 探讨6个微小RNA(microRNA)多态性位点(rs11614913，microRNA-196a2；rs3746444，hsa-mir-499；rs2910164，microRNA-146a；rs2292832，microRNA-149；rs6505162，microRNA-423；rs895819，microRNA-27a)与中国女性乳腺癌发病风险及病理特征的相关性。方法 采用病例对照研究，选取350例乳腺癌患者为病例组及350名正常女性为对照组，采用Massarray SNP技术检测6个多态性位点的基因型，采用免疫组化法检测乳腺癌组织中的雌激素受体及孕激素受体表达，分析各基因型与乳腺癌发生的关系及与乳腺癌病理参数的关系。结果 rs3746444GG基因型在病例组中(OR=1.71，95%CI:1.16~2.52)的频率显著高于对照组。分层分析结果显示，在绝经后的人群中，rs3746444GG基因型病例组中的频率(OR=2.19，95%CI:1.16~4.15)显著高于对照组。rs3746444GG基因型在肿瘤分期0～Ⅱ组(OR=1.92，95%CI:1.17~3.13)、淋巴结转移组(OR=2.20，95%CI:1.28~3.79)、孕激素受体阴性组中(OR=2.19，95%CI:1.25~3.82)分布频率均显著高于正常对照组。在绝经后人群中，rs2910164GG基因型在病例组中(OR=2.19，95%CI:1.16~4.15)的分布频率显著高于对照组。而在绝经前的人群中，rs2910164GG基因型在病例组中(OR=0.49，95%CI:0.25~0.98)的分布频率显著低于对照组。结论 rs3746444GG基因型与乳腺癌发病风险相关，特别是在绝经后人群中风险更高。rs2910164GG基因型与乳腺癌的发病风险相关性与妇女是否绝经有关。
Abstract:
Objective To investigate the relationship between the polymorphisms in microRNAs (rs11614913， miRNA-196a2； rs3746444， hsa-mir-499； rs2910164， miRNA-146a； rs2292832， miRNA-149； rs6505162， miRNA-423； rs895819， miRNA-27a) and risk and pathologic characterize of breast cancer in Chinese female population. Methods A total of 350 patients with breast cancer and 350 health controls were enrolled for this case-controls study. Sequenom MassARRAY was applied to detect the polymorphisms， and the immunohistochemistry assay was used to measure the expression of estrogen receptor (ER) and progesterone receptor (PR). Logistic regression was applied to assess the association between the polymorphisms and risk and pathologic characterize of breast cancer. Results The results showed that the frequency of 3746444GG genotype(OR=1.71， 95% CI: 1.16-2.52) was higher in cases than that in health controls. Sub-group analysis revealed that， in the subgroup of women with postmenopausal status， the frequency of 3746444GG genotype (OR=2.19， 95% CI: 1.16-4.15) was higher in cases than that in health controls. Moreover， the frequency of 3746444GG was higher in subgroup of patients with tumor stage 0-Ⅱ(OR=1.92， 95% CI: 1.17-3.13)， lymph node involvement(OR=2.20， 95% CI: 1.28-3.79)， negative PR expression (OR=2.19， 95% CI: 1.25-3.82) than that in health controls， respectively. In addition， rs2910164GG genotype(OR=2.19， 95% CI: 1.16-4.15) was associated with increased breast cancer risk in the postmenopausal status sub-cohort， while， rs2910164GG genotype (OR=0.49， 95% CI: 0.25-0.98) was associated with decreased breast cancer risk in the premenopausal status sub-cohort. Conclusion rs3746444GG genotype was associated with breast cancer risk， especiallyfor women who with postmenopausal status. The risk of rs2910164GG for breast cancer was modified by the postmenopausal status.

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备注/Memo

备注/Memo:
国家自然科学基金(81200401)；南京市医学科学技术发展项目(JQX13003，QRX11254，QYK11175)
更新日期/Last Update: 2015-01-20