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[1]林慧,陈佳菁,郭志锋,等.miR-9-5p在乳腺癌组织中的表达与生物信息学分析[J].医学研究与战创伤救治(原医学研究生学报),2023,25(1):23-27.[doi:10.3969/j.issn.1672-271X.2023.01.005]
　LIN Hui,CHEN Jiajing,GUO Zhifeng,et al.Expression and bioinformatic analysis of miR-9-5p in breast cancer tissues[J].JOURNAL OF MEDICALRESEARCH —COMBAT TRAUMA CARE,2023,25(1):23-27.[doi:10.3969/j.issn.1672-271X.2023.01.005]
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miR-9-5p在乳腺癌组织中的表达与生物信息学分析()
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《医学研究与战创伤救治》（原医学研究生学报）[ISSN:1672-271X/CN:32-1713/R]

卷:
第25卷
期数:
2023年1期
页码:
23-27
栏目:
临床研究
出版日期:
2023-05-10

文章信息/Info

Title:
Expression and bioinformatic analysis of miR-9-5p in breast cancer tissues
作者:
林慧; 陈佳菁; 郭志锋; 陈燕红; 邱建龙
作者单位：362000泉州，解放军联勤保障部队第九一○医院病理科（林慧、陈佳菁、郭志锋、陈燕红、邱建龙)
Author(s):
LIN Hui; CHEN Jiajing; GUO Zhifeng; CHEN Yanhong; QIU Jianlong
（Department of Pathology，the 910th Hospital of the Joint Logistics Support Force，PLA，Quanzhou 362000，Fujian，China）
关键词:
miR-9-5p; 乳腺癌; 激素受体; 靶基因; 生物信息学
Keywords:
miR-9-5p; breast cancer; hormone receptor; target gene; bioinformatics
分类号:
R737.9
DOI:
10.3969/j.issn.1672-271X.2023.01.005
文献标志码:
A
摘要:
目的探讨miR-9-5p在乳腺癌组织中的表达及其与临床病理特征的关系，预测miR-9-5p靶基因并分析其在乳腺癌进展中的作用。 方法通过实时荧光定量PCR检测83例乳腺癌和癌旁正常组织中miR-9-5p的相对表达量。分析miR-9-5p表达水平与乳腺癌患者临床病理特征的关系。使用生物信息学方法预测miR-9-5p的靶基因并筛选关键节点基因，并对靶基因进行富集分析。结果miR-9-5p在乳腺癌组织中的表达高于癌旁正常组织[1.45（0.41，4.72）vs 1.01（0.35，3.68），P＜0.01]，其表达水平与乳腺癌组织学级别、肿瘤大小、雌激素受体状态、孕激素受体状态、Ki67增殖指数和分子分型有关（P＜0.05），与年龄、肿瘤部位、TNM分期、淋巴结转移情况和人表皮生长因子受体2状态无关（P＞0.05）。miR-9-5p在80.95%（17/21）的三阴性乳腺癌、32.56%（14/43）的管腔型乳腺癌呈高表达，差异具有统计学意义（P＜0.05）。生物信息学方法预测的靶基因有138个，并筛选出SIRT1、BCL6、FOXP1等关键节点基因。miR-9-5p靶基因功能集中于细胞对激素刺激的反应、雄激素受体信号通路负调控、生长调控等方面。结论miR-9-5p过表达与乳腺癌进展关系密切，参与激素受体相关的信号通路调控可能是重要的作用机制。
Abstract:
ObjectiveTo investigate the expression level of miR-9-5p in breast cancer and its relationship with clinicopathological features, predict the target gene of miR-9-5p and analyze its role in the progression of breast cancer.MethodsThe relative expression of miR-9-5p in 83 cases of breast cancer and adjacent normal tissues was detected by real-time fluorescent quantitative PCR. The relationship between miR-9-5p expression level and clinicopathological features of breast cancer patients was analyzed. Bioinformatic methods were utilized to predict the target genes of miR-9-5p and screen the key node genes, and enrichment analysis of target genes was performed.ResultsThe expression of miR-9-5p in breast cancer tissues was higher than that in adjacent normal tissues[1.45（0.41, 4.72）vs 1.01（0.35, 3.68）, P＜0.01]. The expression level was correlated with histological grade, tumor size, estrogen receptor status, progesterone receptor status, Ki67 proliferation index and molecular typing of breast cancer (P<0.05), but not with age, tumor location, TNM stage, lymph node metastasis and human epidermal growth factor receptor 2 status (P>0.05). MiR-9-5p was highly expressed in 80.95% (17/21) of triple-negative breast cancer and 32.56% (14/43) of luminal breast cancer, and the difference was statistically significant (P<0.05). There are 138 target genes predicted by bioinformatic methods, and key node genes such as SIRT1, BCL6, FOXP1 were screened. The function of miR-9-5p target gene focuses on cellular response to hormone stimulation, negative regulation of androgen receptor signaling pathway, growth regulation and so on.ConclusionmiR-9-5p overexpression is closely related to the progression of breast cancer, and the regulation of hormone receptor-related signaling pathways may be an important mechanism.

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备注/Memo

备注/Memo:
基金项目：泉州市科技计划项目（2018N132S）
更新日期/Last Update: 2023-04-19